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Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Understanding inner self-states in eating disorders: preliminary findings from a structured elicitation study. [PDF]

open access: yesEat Weight Disord
Meneguzzo P   +7 more
europepmc   +1 more source

Authoritarian parenting at 4.5 years predicts children's cheating at 6 years. [PDF]

open access: yesChild Dev
Yu L   +11 more
europepmc   +1 more source

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