3D-Printing for Analytical Ultracentrifugation. [PDF]
PLoS ONE, 2016 Analytical ultracentrifugation (AUC) is a classical technique of physical biochemistry providing information on size, shape, and interactions of macromolecules from the analysis of their migration in centrifugal fields while free in solution.
Abhiksha Desai +4 more
doaj +22 more sources
Optimization of Membrane Protein TmrA Purification Procedure Guided by Analytical Ultracentrifugation [PDF]
Membranes, 2021 Membrane proteins are involved in various cellular processes. However, purification of membrane proteins has long been a challenging task, as membrane protein stability in detergent is the bottleneck for purification and subsequent analyses.
Dongdong Li +3 more
doaj +2 more sources
Molecular Size Analysis of Recombinant Importin-histone Complexes Using Analytical Ultracentrifugation [PDF]
Bio-Protocol, 2020 Histones constitute the protein components of nucleosomes. Despite their small sizes, histones do not diffuse through the nuclear pore complex. Instead, they are transported to the nucleus by importins, either alone or in complex with histone chaperones.
Abhilash Padavannil +2 more
doaj +2 more sources
Moving analytical ultracentrifugation software to a good manufacturing practices (GMP) environment. [PDF]
PLoS Computational Biology, 2020 Recent advances in instrumentation have moved analytical ultracentrifugation (AUC) closer to a possible validation in a Good Manufacturing Practices (GMP) environment. In order for AUC to be validated for a GMP environment, stringent requirements need to
Alexey Savelyev +3 more
doaj +2 more sources
Structural Insights into PROTAC Complex Formation from Analytical Ultracentrifugation and Hydrodynamic Modeling [PDF]
Structural DynamicsTargeted protein degradation (TPD) has garnered appreciable interest in drug discovery due to its unique mechanism of action – degradation of a target in an event-driven manner, instead of traditional occupancy-driven inhibitor-based therapies.
Alexander E Yarawsky, Lake N Paul
doaj +2 more sources
Sedimentation Velocity Analytical Ultracentrifugation of Oxidized Recombinant Full-Length Factor VIII [PDF]
Frontiers in Immunology, 2020 Anti-drug antibodies to coagulation factor VIII (fVIII), often termed inhibitors, present the greatest economical and treatment related obstacle in the management of hemophilia A.
Philip M. Zakas +4 more
doaj +2 more sources
Analytical Ultracentrifugation as a Matrix-Free Probe for the Study of Kinase Related Cellular and Bacterial Membrane Proteins and Glycans [PDF]
Molecules, 2021 Analytical ultracentrifugation is a versatile approach for analysing the molecular mass, molecular integrity (degradation/aggregation), oligomeric state and association/dissociation constants for self-association, and assay of ligand binding of kinase ...
Stephen E. Harding
doaj +2 more sources
Quantitation of Enterovirus A71 Empty and Full Particles by Sedimentation Velocity Analytical Ultracentrifugation [PDF]
VirusesThe enterovirus A71 (EV71) inactivated vaccine is an effective intervention to control the spread of the virus and prevent EV71-associated hand, foot, and mouth disease (HFMD). It is widely administered to infants and children in China.
Anna Yang +14 more
doaj +2 more sources
A radial calibration window for analytical ultracentrifugation. [PDF]
PLoS ONE, 2018 Analytical ultracentrifugation (AUC) is a first-principles based method for studying macromolecules and particles in solution by monitoring the evolution of their radial concentration distribution as a function of time in the presence of a high ...
Thomas LeBrun +7 more
doaj +2 more sources
Simple Determination of Gold Nanocrystal Dimensions by Analytical Ultracentrifugation via Surface Ligand-Solvent Density Matching [PDF]
Nanomaterials, 2021 Analytical ultracentrifugation (AUC) is a powerful technique to observe colloidal nanocrystals (NCs) directly in solution and obtain critical information about their physical-chemical properties.
Guillermo González-Rubio +5 more
doaj +2 more sources