Analytical Ultracentrifugation as a Matrix-Free Probe for the Study of Kinase Related Cellular and Bacterial Membrane Proteins and Glycans [PDF]
Molecules, 2021 Analytical ultracentrifugation is a versatile approach for analysing the molecular mass, molecular integrity (degradation/aggregation), oligomeric state and association/dissociation constants for self-association, and assay of ligand binding of kinase ...
Stephen E. Harding
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A radial calibration window for analytical ultracentrifugation. [PDF]
PLoS ONE, 2018 Analytical ultracentrifugation (AUC) is a first-principles based method for studying macromolecules and particles in solution by monitoring the evolution of their radial concentration distribution as a function of time in the presence of a high ...
Thomas LeBrun +7 more
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Sedimentation Velocity Analytical Ultracentrifugation of Oxidized Recombinant Full-Length Factor VIII [PDF]
Frontiers in Immunology, 2020 Anti-drug antibodies to coagulation factor VIII (fVIII), often termed inhibitors, present the greatest economical and treatment related obstacle in the management of hemophilia A.
Philip M. Zakas +4 more
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Molecular Size Analysis of Recombinant Importin-histone Complexes Using Analytical Ultracentrifugation [PDF]
Bio-Protocol, 2020 Histones constitute the protein components of nucleosomes. Despite their small sizes, histones do not diffuse through the nuclear pore complex. Instead, they are transported to the nucleus by importins, either alone or in complex with histone chaperones.
Abhilash Padavannil +2 more
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3D-Printing for Analytical Ultracentrifugation. [PDF]
PLoS ONE, 2016 Analytical ultracentrifugation (AUC) is a classical technique of physical biochemistry providing information on size, shape, and interactions of macromolecules from the analysis of their migration in centrifugal fields while free in solution.
Abhiksha Desai +4 more
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Analytical ultracentrifugation in structural biology. [PDF]
Biophys Rev, 2018 Researchers in the field of structural biology, especially X-ray crystallography and protein nuclear magnetic resonance, are interested in knowing as much as possible about the state of their target protein in solution. Not only is this knowledge relevant to studies of biological function, it also facilitates determination of a protein structure using ...
Unzai S.
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Optimization of Membrane Protein TmrA Purification Procedure Guided by Analytical Ultracentrifugation [PDF]
Membranes, 2021 Membrane proteins are involved in various cellular processes. However, purification of membrane proteins has long been a challenging task, as membrane protein stability in detergent is the bottleneck for purification and subsequent analyses.
Dongdong Li +3 more
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Nanodisc characterization by analytical ultracentrifugation
Nanotechnology Reviews, 2017 Due to their unique properties, tunable size, and ability to provide a near native lipid environment, nanodiscs have found widespread use for the structural and functional studies of reconstituted membrane proteins.
Inagaki Sayaka, Ghirlando Rodolfo
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Moving analytical ultracentrifugation software to a good manufacturing practices (GMP) environment. [PDF]
PLoS Computational Biology, 2020 Recent advances in instrumentation have moved analytical ultracentrifugation (AUC) closer to a possible validation in a Good Manufacturing Practices (GMP) environment. In order for AUC to be validated for a GMP environment, stringent requirements need to
Alexey Savelyev +3 more
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Structural Insights into PROTAC Complex Formation from Analytical Ultracentrifugation and Hydrodynamic Modeling [PDF]
Structural DynamicsTargeted protein degradation (TPD) has garnered appreciable interest in drug discovery due to its unique mechanism of action – degradation of a target in an event-driven manner, instead of traditional occupancy-driven inhibitor-based therapies.
Alexander E Yarawsky, Lake N Paul
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