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Analytical Ultracentrifugation as a Matrix-Free Probe for the Study of Kinase Related Cellular and Bacterial Membrane Proteins and Glycans [PDF]

open access: goldMolecules, 2021
Analytical ultracentrifugation is a versatile approach for analysing the molecular mass, molecular integrity (degradation/aggregation), oligomeric state and association/dissociation constants for self-association, and assay of ligand binding of kinase ...
Stephen E. Harding
doaj   +4 more sources

A radial calibration window for analytical ultracentrifugation. [PDF]

open access: goldPLoS ONE, 2018
Analytical ultracentrifugation (AUC) is a first-principles based method for studying macromolecules and particles in solution by monitoring the evolution of their radial concentration distribution as a function of time in the presence of a high ...
Thomas LeBrun   +7 more
doaj   +4 more sources

Sedimentation Velocity Analytical Ultracentrifugation of Oxidized Recombinant Full-Length Factor VIII [PDF]

open access: goldFrontiers in Immunology, 2020
Anti-drug antibodies to coagulation factor VIII (fVIII), often termed inhibitors, present the greatest economical and treatment related obstacle in the management of hemophilia A.
Philip M. Zakas   +4 more
doaj   +3 more sources

Molecular Size Analysis of Recombinant Importin-histone Complexes Using Analytical Ultracentrifugation [PDF]

open access: greenBio-Protocol, 2020
Histones constitute the protein components of nucleosomes. Despite their small sizes, histones do not diffuse through the nuclear pore complex. Instead, they are transported to the nucleus by importins, either alone or in complex with histone chaperones.
Abhilash Padavannil   +2 more
doaj   +4 more sources

3D-Printing for Analytical Ultracentrifugation. [PDF]

open access: yesPLoS ONE, 2016
Analytical ultracentrifugation (AUC) is a classical technique of physical biochemistry providing information on size, shape, and interactions of macromolecules from the analysis of their migration in centrifugal fields while free in solution.
Abhiksha Desai   +4 more
doaj   +5 more sources

Analytical ultracentrifugation in structural biology. [PDF]

open access: bronzeBiophys Rev, 2018
Researchers in the field of structural biology, especially X-ray crystallography and protein nuclear magnetic resonance, are interested in knowing as much as possible about the state of their target protein in solution. Not only is this knowledge relevant to studies of biological function, it also facilitates determination of a protein structure using ...
Unzai S.
europepmc   +6 more sources

Optimization of Membrane Protein TmrA Purification Procedure Guided by Analytical Ultracentrifugation [PDF]

open access: yesMembranes, 2021
Membrane proteins are involved in various cellular processes. However, purification of membrane proteins has long been a challenging task, as membrane protein stability in detergent is the bottleneck for purification and subsequent analyses.
Dongdong Li   +3 more
doaj   +2 more sources

Nanodisc characterization by analytical ultracentrifugation

open access: hybridNanotechnology Reviews, 2017
Due to their unique properties, tunable size, and ability to provide a near native lipid environment, nanodiscs have found widespread use for the structural and functional studies of reconstituted membrane proteins.
Inagaki Sayaka, Ghirlando Rodolfo
doaj   +2 more sources

Moving analytical ultracentrifugation software to a good manufacturing practices (GMP) environment. [PDF]

open access: yesPLoS Computational Biology, 2020
Recent advances in instrumentation have moved analytical ultracentrifugation (AUC) closer to a possible validation in a Good Manufacturing Practices (GMP) environment. In order for AUC to be validated for a GMP environment, stringent requirements need to
Alexey Savelyev   +3 more
doaj   +2 more sources

Structural Insights into PROTAC Complex Formation from Analytical Ultracentrifugation and Hydrodynamic Modeling [PDF]

open access: yesStructural Dynamics
Targeted protein degradation (TPD) has garnered appreciable interest in drug discovery due to its unique mechanism of action – degradation of a target in an event-driven manner, instead of traditional occupancy-driven inhibitor-based therapies.
Alexander E Yarawsky, Lake N Paul
doaj   +2 more sources

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