Results 101 to 110 of about 21,890 (235)

Noninvasive Assessment of β‐Secretase Activity Through Click Chemistry‐Mediated Enrichment of Neuronal Extracellular Vesicles to Detect Alzheimer's Disease

Advanced Science, EarlyView.
This study presents the NEV β‐secretase activity assay, a groundbreaking method for noninvasive evaluation of β‐secretase activity in Alzheimer's disease (AD) patients, enabling the generation of individualized β‐secretase activity profiles.
Hyoyong Kim, Junseok Lee, Audrey Qian, You‐Ren Ji, Ryan Zhang, Qixin Hu, Christopher Kazu Williams, Han‐Yu Chuang, Matthew D. Smalley, Yaya Xu, Liang Gao, Mary C. Mayo, Ting Zhang, Edwin M. Posadas, Zaldy S. Tan, Harry V. Vinters, Keith Vossel, Shino Magaki, Yazhen Zhu, Hsian‐Rong Tseng   +19 more
wiley   +1 more source

A structural basis for IκB kinase 2 activation via oligomerization-dependent trans auto-phosphorylation. [PDF]

, 2013
Activation of the IκB kinase (IKK) is central to NF-κB signaling. However, the precise activation mechanism by which catalytic IKK subunits gain the ability to induce NF-κB transcriptional activity is not well understood.
Fusco, Amanda J, Ghosh, Gourisankar, Hauenstein, Arthur V, Hoffmann, Alexander, Huang, De-Bin, Huxford, Tom, Kim, Youngchang, Polley, Smarajit, Schröfelbauer, Bärbel, Verma, Inder M, Vu, Don, Zhong, Xiangyang   +11 more
core   +2 more sources

M2 Macrophage‐Derived Extracellular Vesicles Reprogram Immature Neutrophils into Anxa1hi Neutrophils to Enhance Inflamed Bone Regeneration

Advanced Science, EarlyView.
This study reveals that M2‐EVs mitigate periodontitis‐induced bone resorption by modulating neutrophil fate. Single‐cell RNA sequencing identified an Anxa1hi neutrophil subpopulation with pro‐reparative properties. M2‐EVs disrupt neutrophil maturation, promoting this subpopulation through key reprogramming genes (Acvrl1, Fpr2). These findings highlight
Yufei Yao, Yijia Yin, Fangyuan Shuai, Waishan Lam, Tao Zhou, Yaxin Xie, Xuesong He, Xianglong Han   +7 more
wiley   +1 more source

A Dual Approach with Organoid and CRISPR Screening Reveals ERCC6 as a Determinant of Cisplatin Resistance in Osteosarcoma

Advanced Science, EarlyView.
This study utilizes patient‐derived osteosarcoma organoids and CRISPR screening to identify ERCC6 and HNRNPM as key mediators of cisplatin resistance via the PI3K/AKT pathway and BAX alternative splicing. These findings provide novel insights and potential therapeutic targets for overcoming chemoresistance in osteosarcoma.
Ruiling Xu, Sai Zhu, Wenchao Zhang, Haodong Xu, Chao Tu, Honghui Wang, Lu Wang, Na He, Tang Liu, Xiaoning Guo, Xiaolei Ren, Zhihong Li   +11 more
wiley   +1 more source

Aromatic residues in the C-terminal helix of human apoC-I mediate phospholipid interactions and particle morphology[S]

Journal of Lipid Research, 2009
Human apolipoprotein C-I (apoC-I) is an exchangeable apolipoprotein that binds to lipoprotein particles in vivo. In this study, we employed a LC-MS/MS assay to demonstrate that residues 38–51 of apoC-I are significantly protected from proteolysis in the ...
Patrick F. James, Con Dogovski, Renwick C.J. Dobson, Michael F. Bailey, Kenneth N. Goldie, John A. Karas, Denis B. Scanlon, Richard A.J. O’Hair, Matthew A. Perugini   +8 more
doaj  

Autoinhibition of the formin Cappuccino in the absence of canonical autoinhibitory domains. [PDF]

, 2012
Formins are a conserved family of proteins known to enhance actin polymerization. Most formins are regulated by an intramolecular interaction. The Drosophila formin, Cappuccino (Capu), was believed to be an exception.
Bor, Batbileg, Phillips, Martin L, Quinlan, Margot E, Vizcarra, Christina L   +3 more
core   +2 more sources

Neutrophil Macrophage Crosstalk via Extracellular Vesicles Drives Reverse Migration in a Fully Human Model of Wound Healing

Advanced Science, EarlyView.
A novel microphysiological system that can mimic sterile or non‐sterile injury demonstrates that macrophage‐derived extracellular vesicles modulate neutrophil migratory responses to these different wound states. The model showed that resolution of inflammation through neutrophil reverse migration is driven by IL‐8 derived from extracellular vesicles ...
Kehinde Adebayo Babatunde, Oluwadamilola Fatimat Babatunde, Adeel Ahmed, Wilmara Salgado‐Pabon, David J Beebe, Sheena C. Kerr   +5 more
wiley   +1 more source

Kindlin Regulates Mechanosensitive Activation and Adhesion Assembly of Integrin beta6

Advanced Science, EarlyView.
Extracellular matrix compliance plays a critical role in the regulation of integrin‐mediated adhesion and cell motility. Here, it is reported that strengthening the kindlin2‐integrin β6 interaction overcomes the dependence on extracellular substrate stiffness and promotes integrin β6‐mediated cell migration.
Wan Ning Lee, Jiamin Li, Nan‐Peng Chen, Cheng‐han Yu   +3 more
wiley   +1 more source

Transient Interdomain Interactions Modulate the Monomeric Structural Ensemble and Self‐Assembly of Huntingtin Exon 1

Advanced Science, EarlyView.
Polyglutamine (polyQ) tract expansion (≥ 36 amino acids) within the N‐terminal region of the Huntingtin protein (Httex1) causes Huntington's disease (HD), for which the underlying causes are not well‐understood. The authors performed computer simulations to understand the cause of HD at the molecular level.
Priyesh Mohanty, Tien Minh Phan, Jeetain Mittal   +2 more
wiley   +1 more source

Random PCR and ultracentrifugation increases sensitivity and throughput of VIDISCA for screening of pathogens in clinical specimens

Journal of Infection in Developing Countries, 2010
Introduction: Virus discovery based on cDNA-AFLP (VIDISCA) is a sequence-independent virus discovery method that was recently developed and successfully used to characterize unknown viruses in cell cultures. Its applicability, however, is limited by its
Le Van Tan, H. Rogier van Doorn, Lia van der Hoek, Vo Minh Hien, Maarten F. Jebbink, Do Quang Ha, Jeremy Farrar, Nguyen Van Vinh Chau, Menno D. de Jong   +8 more
doaj   +1 more source