Molecules, 2021 Analytical ultracentrifugation is a versatile approach for analysing the molecular mass, molecular integrity (degradation/aggregation), oligomeric state and association/dissociation constants for self-association, and assay of ligand binding of kinase ...
Stephen E. Harding
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Frontiers in Bioengineering and Biotechnology, 2021 We observed differential infectivity and product yield between two recombinant chimpanzee adenovirus C68 constructs whose primary difference was genome length.
Elise K. Mullins +13 more
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FolX from Pseudomonas aeruginosa is octameric in both crystal and solution [PDF]
, 2012 FolX encodes an epimerase that forms one step of the tetrahydrofolate biosynthetic pathway, which is of interest as it is an established target for important drugs.
Achari +28 more
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Frontiers in Immunology, 2021 Complement Factor H (CFH), with 20 short complement regulator (SCR) domains, regulates the alternative pathway of complement in part through the interaction of its C-terminal SCR-19 and SCR-20 domains with host cell-bound C3b and anionic oligosaccharides.
Orla M. Dunne +12 more
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Protein concentration of synovial fluid in chronic rheumatoid arthritis. Estimation of protein in the synovial fluid of chronic rheumatoid arthritis by gel filtration and paper electrophoresis [PDF]
, 1968 For the purpose to reveal the characteris6cs of the synovial fluid of the chronic rheumatoid arthritis the proteins of the synovial fluid and blood serum have been analysed by employing the methods of electrophoresis, gel filtration on Sephadex G-200 ...
Seno, Takashi
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Analytical ultracentrifugation of colloids
Nanoscale, 2010 In this contribution the use of Analytical Ultracentrifugation (AUC) for the modern analysis of colloids is reviewed. Since AUC is a fractionation technique, distributions of the sedimentation coefficient, particle size and shape, molar mass and density can be obtained for particle sizes spanning the entire colloidal range.
Planken, Karel L., Cölfen, Helmut
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Moving analytical ultracentrifugation software to a good manufacturing practices (GMP) environment.
PLoS Computational Biology, 2020 Recent advances in instrumentation have moved analytical ultracentrifugation (AUC) closer to a possible validation in a Good Manufacturing Practices (GMP) environment. In order for AUC to be validated for a GMP environment, stringent requirements need to
Alexey Savelyev +3 more
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Inhibition by small-molecule ligands of formation of amyloid fibrils of an immunoglobulin light chain variable domain. [PDF]
, 2015 Overproduction of immunoglobulin light chains leads to systemic amyloidosis, a lethal disease characterized by the formation of amyloid fibrils in patients' tissues. Excess light chains are in equilibrium between dimers and less stable monomers which can
Brumshtein, Boris +7 more
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Biochemical analysis of TssK, a core component of the bacterial Type VI secretion system, reveals distinct oligomeric states of TssK and identifies a TssK–TssFG subcomplex [PDF]
, 2014 Gram-negative bacteria use the Type VI secretion system (T6SS) to inject toxic proteins into rival bacteria or eukaryotic cells. However, the mechanism of the T6SS is incompletely understood. In the present study, we investigated a conserved component of
Alan R. Prescott +60 more
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Influence of Mie scattering on nanoparticles with different particle sizes and shapes: Photometry and analytical ultracentrifugation with absorption optics [PDF]
Journal of the Serbian Chemical Society, 2005 Nanoparticles are used in large quantities for very different applications. A precise determination of the diameter and the particle size distribution which is responsible for the application properties is therefore essential.
Lechner M.D.
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