Results 211 to 220 of about 47,111 (272)

Adipose‐derived small extracellular vesicles exacerbate microvascular dysfunction in heart failure with preserved ejection fraction via mmu‐miR‐582‐3p‐mediated Rap1b suppression

Interdisciplinary Medicine, EarlyView.
This study reveals that small extracellular vesicles (sEVs) from visceral fat compromise coronary microcirculation in a mouse model of heart failure with preserved ejection fraction (HFpEF). The SGLT2 inhibitor canagliflozin emerges as a therapeutic strategy, improving cardiac function and endothelial health.
Lu Chen, Qiuhan Wang, Mengjia Shen, Mingjue Li, Yingqi Zhu, Cankun Zheng, Siyuan Ma, Hairuo Lin, Xiaokang Fu, Rui Zhang, Jianping Bin, Yulin Liao, Qiancheng Wang   +12 more
wiley   +1 more source

Weak IgG self- and hetero-association characterized by fluorescence analytical ultracentrifugation. [PDF]

Protein Sci, 2018
Yang D, Correia JJ, Stafford Iii WF, Roberts CJ, Singh S, Hayes D, Kroe-Barrett R, Nixon A, Laue TM.   +8 more
europepmc   +1 more source

Plasmonics‐Enhanced Characterization of Cervid PrP (87–114) Fragment Aggregates in Solution

Journal of Biophotonics, EarlyView.
Multimodal nanophotonics platform combining plasmon‐enhanced imaging, label‐free SERS, and optical staining reveals new insights into the aggregation of the cervid PrP (87–114) fragment, an amyloidogenic sequence linked to chronic wasting disease susceptibility.
Shinki Midha, Aishwarya Sriraman, Lyudmyla Dorosh, Holger Wille, Maria Stepanova   +4 more
wiley   +1 more source

Multi-wavelength analytical ultracentrifugation of human serum albumin complexed with porphyrin. [PDF]

Eur Biophys J, 2018
Johnson CN, Gorbet GE, Ramsower H, Urquidi J, Brancaleon L, Demeler B.   +5 more
europepmc   +1 more source

Profiling Protein Citrullination in Extracellular Vesicles by Single‐Molecule Detection Using Direct Stochastic Optical Reconstruction Microscopy

Journal of Biophotonics, EarlyView.
This study identifies post‐translationally citrullinated/deiminated protein cargoes co‐localised to EV subtypes, using super resolution microscopy and dSTORM imaging. The findings expand current understanding of post‐translational modifications in EV‐mediated cellular communication.
Sarah R. Needham, Benjamin M. Davis, Pinar Uysal‐Onganer, Daniel J. Rolfe, Mariya Hristova, Igor Kraev, Jameel M. Inal, Sigrun Lange   +7 more
wiley   +1 more source

Capture of adeno‐associated viruses from clarified lysate by continuous flow ultracentrifugation: a comparative study of iodixanol and sucrose gradients

Journal of Chemical Technology &Biotechnology, EarlyView.
Abstract BACKGROUND Adeno‐Associated Virus vectors are widely used in gene therapy due to their safety and efficacy. Despite the interest in their therapeutical application, AAV purification remains a significant challenge. Density gradient ultracentrifugation using cesium chloride, sucrose, or iodixanol gradients are common methods for AAV ...
Sabrina Leigheb, Maral Rahimzadeh, Nikolaus Virgolini, Aida Tleuova, Mahbod Mousavian, Astrid Dürauer, Patricia Pereira Aguilar   +6 more
wiley   +1 more source

Bioaccessibility of anthocyanins and bioactive compounds from Brazilian berries and their food matrix interaction: an in vitro gastrointestinal digestion study coupled to UHPLC‐ESI‐TQD‐MS/MS analysis

Journal of the Science of Food and Agriculture, EarlyView.
Abstract BACKGROUND Several commercial berries have been examined for anthocyanin composition, bioaccessibility, and bioactive effects, while Brazilian berries remain underexplored. This study aimed to access the effect of in vitro gastrointestinal digestion on anthocyanin bioaccessibility, stability, and antioxidant potential in black pitanga (Eugenia
Paulo Berni, Laís R Zandoná, Patrícia B Berilli, Livia Reguengo, Giulia Bonhin, Daniela CS Baldan, Mário R Maróstica Jr   +6 more
wiley   +1 more source

Sedimentation velocity analytical ultracentrifugation for characterization of therapeutic antibodies. [PDF]

Biophys Rev, 2018
Uchiyama S, Noda M, Krayukhina E.
europepmc   +1 more source

Extracellular Vesicle‐Mediated Communication Between Anterior Cruciate Ligament and Bone Marrow Cells Modulates Hamstring Tenocyte Behavior and Apoptosis

The Kaohsiung Journal of Medical Sciences, EarlyView.
ABSTRACT Following anterior cruciate ligament (ACL) reconstruction, enhancing hamstring tenocyte activity and minimizing apoptosis are critical for preventing graft failure and promoting ligamentization. This study investigated the therapeutic potential of extracellular vesicles (EVs) derived from a coculture of ACL remnant cells and bone marrow ...
Hon‐Lok Lo, Shih‐Hao Huang, Ting‐Hsuan Dai, Shun‐Cheng Wu, Cheng‐Jung Ho, Cheng‐Chang Lu   +5 more
wiley   +1 more source

Challenges and Opportunities in State‐of‐the‐Art Proteomics Analysis for Biomarker Development From Plasma Extracellular Vesicles

PROTEOMICS, EarlyView.
ABSTRACT Extracellular vesicles (EVs) are membrane‐bound particles secreted by cells, playing crucial roles in intercellular communication. The composition of EVs can undergo changes in response to stress and disease conditions, making them excellent biomarker candidates.
Panshak P. Dakup, Ivo Diaz Ludovico, Youngki You, Chaitra Rao, Javier Flores, Lisa M. Bramer, Marian Rewers, Bobbie‐Jo M. Webb‐Robertson, Thomas O. Metz, Raghavendra G. Mirmira, Emily K. Sims, Ernesto S. Nakayasu   +11 more
wiley   +1 more source