Results 31 to 40 of about 25,111 (265)

The isolation of lipoproteins from human plasma by ultracentrifugation in zonal rotors

Journal of Lipid Research, 1971
The major classes of lipoproteins were isolated from human plasma by ultracentrifugation in continuous density gradients using the Ti-14 and Ti-15 zonal rotors.
HENRY G. WILCOX, DONALD C. DAVIS, MURRAY HEIMBERG   +2 more
doaj   +1 more source

Analytical band centrifugation for the separation and quantification of empty and full AAV particles

Molecular Therapy: Methods & Clinical Development, 2021
Analytical band centrifugation (ABC) was first developed for the separation of macromolecules in centrifugation cells ~60 years ago. Since its development, ABC has been predominantly utilized to study macromolecular interactions or chemical reactions ...
Harshit Khasa, Greg Kilby, Xiaoyu Chen, Chunlei Wang   +3 more
doaj   +1 more source

Structural Characterization of Two Short Unspecific Peroxygenases: Two Different Dimeric Arrangements

Antioxidants, 2022
Unspecific peroxygenases (UPOs) are extracellular fungal enzymes of biotechnological interest as self-sufficient (and more stable) counterparts of cytochrome P450 monooxygenases, the latter being present in most living cells.
Dolores Linde, Elena Santillana, Elena Fernández-Fueyo, Alejandro González-Benjumea, Juan Carro, Ana Gutiérrez, Angel T. Martínez, Antonio Romero   +7 more
doaj   +1 more source

Nanoscale Structure Determination of Murray Valley Encephalitis and Powassan Virus Non-Coding RNAs

Viruses, 2020
Viral infections are responsible for numerous deaths worldwide. Flaviviruses, which contain RNA as their genetic material, are one of the most pathogenic families of viruses. There is an increasing amount of evidence suggesting that their 5’ and 3&#
Tyler Mrozowich, Amy Henrickson, Borries Demeler, Trushar R Patel   +3 more
doaj   +1 more source

Monochromatic multicomponent fluorescence sedimentation velocity for the study of high-affinity protein interactions

eLife, 2016
The dynamic assembly of multi-protein complexes underlies fundamental processes in cell biology. A mechanistic understanding of assemblies requires accurate measurement of their stoichiometry, affinity and cooperativity, and frequently consideration of ...
Huaying Zhao, Yan Fu, Carla Glasser, Eric J Andrade Alba, Mark L Mayer, George Patterson, Peter Schuck   +6 more
doaj   +1 more source

Tannic Acid-Mediated Aggregate Stabilization of Poly(N-vinylpyrrolidone)-b-poly(oligo (ethylene glycol) methyl ether methacrylate) Double Hydrophilic Block Copolymers

Nanomaterials, 2019
The self-assembly of block copolymers in aqueous solution is an important field in modern polymer science that has been extended to double hydrophilic block copolymers (DHBC) in recent years.
Noah Al Nakeeb, Ivo Nischang, Bernhard V.K.J. Schmidt   +2 more
doaj   +1 more source

Characterization of Recombinant Chimpanzee Adenovirus C68 Low and High-Density Particles: Impact on Determination of Viral Particle Titer

Frontiers in Bioengineering and Biotechnology, 2021
We observed differential infectivity and product yield between two recombinant chimpanzee adenovirus C68 constructs whose primary difference was genome length.
Elise K. Mullins, Thomas W. Powers, Jim Zobel, Kory M. Clawson, Lauren F. Barnes, Benjamin E. Draper, Qin Zou, Joseph J. Binder, Stanley Dai, Kun Zhang, Olga Friese, Herbert A. Runnels, Martin F. Jarrold, Lawrence C. Thompson   +13 more
doaj   +1 more source

A centrifugation-based physicochemical characterization method for the interaction between proteins and nanoparticles

Nature Communications, 2016
Thein vivobehaviour of nanoparticles is influenced by the presence of the protein corona. Here, the authors use analytical ultracentrifugation to study the interaction of gold nanoparticles with bovine serum albumin, to gain insight into the corona ...
Ahmet Bekdemir, Francesco Stellacci
doaj   +1 more source

Quantitation of serum lipoproteins by electrophoresis on agarose gel: standardization in lipoprotein concentration units (mg/100 ml) by comparison with analytical ultracentrifugation

Journal of Lipid Research, 1971
Lipoprotein electrophoresis on agarose gel has been modified to allow estimation of the absolute quantity of each fraction. The reproducibility of the method is illustrated by 12 determinations in a single day on serum from one normal subject: mean total
S.B. HULLEY, S.G. COOK, W.S. WILSON, M.Z. NICHAMAN, F.T. HATCH, F.T. LINDGREN   +5 more
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Formation of Nanoclusters in Gold Nucleation

Crystals, 2020
Gold nanoclusters consisting of a specific atom number have gained popularity in research in recent years due to their outstanding properties. Due to their molecule-like behavior, their properties depend strongly on their size.
Cornelia M. Schneider, Helmut Cölfen
doaj   +1 more source