Results 31 to 40 of about 47,111 (272)

Analytical band centrifugation for the separation and quantification of empty and full AAV particles

Molecular Therapy: Methods & Clinical Development, 2021
Analytical band centrifugation (ABC) was first developed for the separation of macromolecules in centrifugation cells ~60 years ago. Since its development, ABC has been predominantly utilized to study macromolecular interactions or chemical reactions ...
Harshit Khasa, Greg Kilby, Xiaoyu Chen, Chunlei Wang   +3 more
doaj   +1 more source

Liquid droplet formation by HP1α suggests a role for phase separation in heterochromatin. [PDF]

, 2017
Gene silencing by heterochromatin is proposed to occur in part as a result of the ability of heterochromatin protein 1 (HP1) proteins to spread across large regions of the genome, compact the underlying chromatin and recruit diverse ligands.
Agard, David A, Burlingame, Alma L, Elnatan, Daniel, Johnston, Jonathan B, Keenen, Madeline M, Larson, Adam G, Narlikar, Geeta J, Redding, Sy, Trnka, Michael J   +8 more
core   +1 more source

Molecular flexibility of citrus pectins by combined sedimentation and viscosity analysis [PDF]

, 2007
The flexibility/rigidity of pectins plays an important part in their structure-function relationship and therefore on their commercial applications in the food and biomedical industries.
Alan Smith, Alvaro Ortega, Axelos, Bohdanecky, Braccini, Chapman, Corredig, Cros, Fishman, Gordon A. Morris, Harding, Harding, Harding, Harding, Hearst, Hearst, Huggins, Jonathan Castile, José García de al Torre, Kraemer, Kratky, Lapasin, Morris, Morris, Morris, Norziah, Noto, Pavlov, Pavlov, Perez, Picout, Pilgrim, Ralet, Ralston, Ridley, Rowe, Schuck, Schuck, Stephen E. Harding, Stockmayer, Tanford, Tombs, Van Buren, Wales, Willats, Wyatt, Yamakawa, Yoo   +47 more
core   +1 more source

Structure and Activation Mechanism of the Drosophila Initiator Caspase Dronc [PDF]

, 2006
Activation of an initiator caspase is essential to the execution of apoptosis. The molecular mechanisms by which initiator caspases are activated remain poorly understood.
Demeler, Borries, Hay, Bruce A., Huh, Jun R., Schirf, Virgil, Shi, Yigong, Yan, Nieng   +5 more
core   +1 more source

pH Dependence and Stoichiometry of Binding to the Fc Region of IgG by the Herpes Simplex Virus Fc Receptor gE-gI [PDF]

, 2004
Herpes simplex virus type 1 encodes two glycoproteins, gE and gI, that form a heterodimer on the surface of virions and infected cells. The gE-gI heterodimer has been implicated in cell-to-cell spread of virus and is a receptor for the Fc fragment of IgG.
Bjorkman, Pamela J., Martin, W. Lance, Sprague, Elizabeth R.   +2 more
core   +1 more source

Monochromatic multicomponent fluorescence sedimentation velocity for the study of high-affinity protein interactions

eLife, 2016
The dynamic assembly of multi-protein complexes underlies fundamental processes in cell biology. A mechanistic understanding of assemblies requires accurate measurement of their stoichiometry, affinity and cooperativity, and frequently consideration of ...
Huaying Zhao, Yan Fu, Carla Glasser, Eric J Andrade Alba, Mark L Mayer, George Patterson, Peter Schuck   +6 more
doaj   +1 more source

An initial event in insect innate immune response: structural and biological studies of interactions between β-1,3-glucan and the N-terminal domain of β-1,3-glucan recognition protein [PDF]

, 2013
In response to invading microorganisms, insect β-1,3-glucan recognition protein (βGRP), a soluble receptor in the hemolymph, binds to the surfaces of bacteria and fungi and activates serine protease cascades that promote destruction of pathogens by means
Amzel L. M., An C., Barral P., Boraston A. B., Brünger A. T., Buchon N., Cavanagh C., Clore G. M., Cornilescu G., Daisuke Takahashi, David VanderVelde, Delaglio F., Deslandes Y., Fabrick J. A., Fabrick J. A., Goddard T. D., Gottar M., Handa N., Hiromasa Y., Hiromasa Y., Hrmova M., Huaien Dai, Jeffrey A. Fabrick, Jiang H., Kanagawa M., Kim Y. S., Laughton A. M., Lim J. H., Ma C., Matskevich A. A., Michael R. Kanost, Mishima Y., Ochiai M., Okuyama K., Peng J. W., Perkins S. J., Ramaswamy Krishnamoorthi, Roh K. B., Schachman H. K., Takahasi K., Wang Y., Weis W. I., Wiseman T., Wishart D. S., Yasuaki Hiromasa, Young S. H.   +45 more
core   +2 more sources

Tannic Acid-Mediated Aggregate Stabilization of Poly(N-vinylpyrrolidone)-b-poly(oligo (ethylene glycol) methyl ether methacrylate) Double Hydrophilic Block Copolymers

Nanomaterials, 2019
The self-assembly of block copolymers in aqueous solution is an important field in modern polymer science that has been extended to double hydrophilic block copolymers (DHBC) in recent years.
Noah Al Nakeeb, Ivo Nischang, Bernhard V.K.J. Schmidt   +2 more
doaj   +1 more source

Variation in the organization and subunit composition of the mammalian pyruvate dehydrogenase complex E2/E3BP core assembly [PDF]

, 2011
The final version of this article is available at the link below.Crucial to glucose homoeostasis in humans, the hPDC (human pyruvate dehydrogenase complex) is a massive molecular machine comprising multiple copies of three distinct enzymes (E1–E3) and an
Alan Cooper, Blass, Brautigam, Brautigam, Chamberlain, Ciszak, Cooper, David Gilbert, Donna P. McGow, Frank, Hipps, Hiromasa, Hodgson, Hoyer, Huang, Izard, J. Gordon Lindsay, Jones, Jung, Konarev, Laue, Lessard, Leung, Lynch, Mande, Margaret A. Nutley, Mayers, McCartney, Olwyn Byron, Peter Kropholler, Philip Callow, Robinson, Sanderson, Schuck, Semenyuk, Smolle, Stanley, Stoops, Svergun, Swetha Vijayakrishnan, Vijayakrishnan, Wagenknecht, Yu, Zhou, Zhou   +44 more
core   +3 more sources

Characterization of Recombinant Chimpanzee Adenovirus C68 Low and High-Density Particles: Impact on Determination of Viral Particle Titer

Frontiers in Bioengineering and Biotechnology, 2021
We observed differential infectivity and product yield between two recombinant chimpanzee adenovirus C68 constructs whose primary difference was genome length.
Elise K. Mullins, Thomas W. Powers, Jim Zobel, Kory M. Clawson, Lauren F. Barnes, Benjamin E. Draper, Qin Zou, Joseph J. Binder, Stanley Dai, Kun Zhang, Olga Friese, Herbert A. Runnels, Martin F. Jarrold, Lawrence C. Thompson   +13 more
doaj   +1 more source