Results 51 to 60 of about 47,111 (272)

RECONSTITUTION OF ALLOPHYCOCYANIN FROM Mastigocladus laminosus WITH ISOLATED LINKER POLYPEPTIDE [PDF]

open access: yes, 1993
The core linker polypeptide Lc 8.9 was isolated from Mastigocladus laminosus and purified on a preparative scale. A method for the reconstitution of allophycocyanin (AP)—linker complexes from isolated polypeptides was developed. The complex (αAP(βAP)3 Lc
Gottschalk, Lothar   +2 more
core   +1 more source

Analytical ultracentrifugation of colloids

open access: yesNanoscale, 2010
In this contribution the use of Analytical Ultracentrifugation (AUC) for the modern analysis of colloids is reviewed. Since AUC is a fractionation technique, distributions of the sedimentation coefficient, particle size and shape, molar mass and density can be obtained for particle sizes spanning the entire colloidal range.
Planken, Karel L., Cölfen, Helmut
openaire   +4 more sources

Bioavailability of Cu and Zn in raw and anaerobically digested pig slurry [PDF]

open access: yes, 2009
The impact of anaerobic digestion on the bioavailability of copper and zinc from pig slurry was assessed. Both chemical and biological approaches were used independently on raw slurry (RS) and anaerobically digested pig slurry (DS).
Cecchi, Marie   +4 more
core   +2 more sources

Substrate specificity of Burkholderia pseudomallei multidrug transporters is influenced by the hydrophilic patch in the substrate‐binding pocket

open access: yesFEBS Letters, EarlyView.
Multidrug transporters BpeB and BpeF from the Gram‐negative pathogen Burkholderia pseudomallei have a hydrophilic patch in their substrate‐binding pocket. Drug susceptibility tests and growth curve analyses using an Escherichia coli recombinant expression system revealed that the hydrophilic patches of BpeB and BpeF are involved in the substrate ...
Ui Okada, Satoshi Murakami
wiley   +1 more source

Bacterial flagellar capping proteins adopt diverse oligomeric states

open access: yeseLife, 2016
Flagella are crucial for bacterial motility and pathogenesis. The flagellar capping protein (FliD) regulates filament assembly by chaperoning and sorting flagellin (FliC) proteins after they traverse the hollow filament and exit the growing flagellum tip.
Sandra Postel   +12 more
doaj   +1 more source

Binding of the kringle‐2 domain of human plasminogen to streptococcal PAM‐type M‐protein causes dissociation of PAM dimers

open access: yesMicrobiologyOpen, 2021
The direct binding of human plasminogen (hPg), via its kringle‐2 domain (K2hPg), to streptococcal M‐protein (PAM), largely contributes to the pathogenesis of Pattern D Group A Streptococcus pyogenes (GAS).
Olawole Ayinuola   +5 more
doaj   +1 more source

Digital detection of exosomes by interferometric imaging [PDF]

open access: yes, 2016
Exosomes, which are membranous nanovesicles, are actively released by cells and have been attributed to roles in cell-cell communication, cancer metastasis, and early disease diagnostics.
Benussi, Luisa   +13 more
core   +2 more sources

The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803

open access: yesFEBS Letters, EarlyView.
We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee   +3 more
wiley   +1 more source

Substitutions in the redox-sensing PAS domain of the NifL regulatory protein define an inter-subunit pathway for redox signal transmission [PDF]

open access: yes, 2011
The Per-ARNT-Sim (PAS) domain is a conserved a/ß fold present within a plethora of signalling proteins from all kingdoms of life. PAS domains are often dimeric and act as versatile sensory and interaction modules to propagate environmental signals to ...
Ayers   +34 more
core   +1 more source

The histone H3K4 demethylase JARID1A directly interacts with haematopoietic transcription factor GATA1 in erythroid cells through its second PHD domain [PDF]

open access: yes, 2020
Chromatin remodelling and transcription factors play important roles in lineage commitment and development through control of gene expression. Activation of selected lineage-specific genes and repression of alternative lineage-affiliated genes results in
Biasutto, Antonio J   +4 more
core   +1 more source

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