Results 81 to 90 of about 47,111 (272)

The ubiK protein is an accessory factor necessary for bacterial Ubiquinone (UQ) biosynthesis and forms a complex with the UQ biogenesis factor UbiJ [PDF]

, 2017
Ubiquinone (UQ), also referred to as coenzyme Q, is a widespread lipophilic molecule in both prokaryotes and eukaryotes in which it primarily acts as an electron carrier.
Aussel, Laurent, Barras, Frédéric, Casadesús Pursals, Josep, Chehade, Mahmoud Hajj, Faivre, Bruno, Fyfe, Cameron David, Hernández Piñero, Sara Belén, Loiseau, Laurent   +7 more
core   +1 more source

Multiphysics‐Driven Assembly of Biomimetic Vesicles

Advanced Materials, EarlyView.
Artificial extracellular vesicles, derived from cell membranes, are manufactured using a multiphysics‐integrated microfluidic platform that combines nanoknife membrane rupture, herringbone chaotic mixing, and acoustothermal modulation. This standardizable workflow enables the predictable control of vesicle formation and therapeutic loading, while ...
Timofei Solodko, Ian Gimino, Aastha Chandiwala, Bayan Alkotoub, Ali Bashiri Dezfouli, Heiko Reith, Asjad Bakhtiar, Rojda Hicsanmaz, Johann Brenner, Christoph J. O. Kaiser, Elena Belli, Shilpi Pandey, Samuel D. Rabkin, Kornelius Nielsch, Gabriele Multhoff, Oliver Hayden, Morteza Hasanzadeh Kafshgari   +16 more
wiley   +1 more source

Cholesterol‐DNA Origami Nanostructures for Synthetic Lipid Rafts Induce Early T Cell Activation Signals

Advanced Materials Interfaces, EarlyView.
Here, we present a nanotechnology approach to construct synthetic lipid rafts on the live T cell membrane, leveraging a versatile DNA origami‐enabled platform named as the “cholesterol nano‐patch” (CNP). Our investigation highlights the effectiveness of DNA nanotechnology in exploring the impact of nanoscale arrangement of cholesterols on the ...
Yunmin Jung, Young‐Joo Kim, Kunwoo Noh, Sunmi Lee, Minsuk Kwak   +4 more
wiley   +1 more source

13b-K and Nirmatrelvir Resistance Mutations of SARS-CoV-2 Main Protease: Structural, Biochemical, and Biophysical Characterization of Free Enzymes and Inhibitor Complexes

Crystals
The SARS-CoV-2 main protease (Mpro) is a well-established target for antiviral drug development. One such inhibitor, nirmatrelvir, in combination with ritonavir as a booster, has already been introduced into the market, under the name Paxlovid.
Haifa El Kilani, Xinyuanyuan Sun, Mohamed Fouad Ibrahim, Ute Curth, Rolf Hilgenfeld   +4 more
doaj   +1 more source

In the quest for new targets for pathogen eradication: the adenylosuccinate synthetase from the bacterium Helicobacter pylori

Journal of Enzyme Inhibition and Medicinal Chemistry, 2018
Adenylosuccinate synthetase (AdSS) is an enzyme at regulatory point of purine metabolism. In pathogenic organisms which utilise only the purine salvage pathway, AdSS asserts itself as a promising drug target. One of these organisms is Helicobacter pylori,
Ante Bubić, Natalia Mrnjavac, Igor Stuparević, Marta Łyczek, Beata Wielgus-Kutrowska, Agnieszka Bzowska, Marija Luić, Ivana Leščić Ašler   +7 more
doaj   +1 more source

Molecular interactions between complement factor H and its heparin and heparan sulphate ligands

Frontiers in Immunology, 2014
Complement Factor H (CFH) is the major regulator of the central complement protein C3b in the alternative pathway of complement activation. A molecular view of the CFH interaction with native heparan sulphate is central for understanding the mechanism ...
Stephen John Perkins, Ka Wai eFung, Sanaullah eKhan   +2 more
doaj   +1 more source

Biocatalytic Nanoregulators Restore Joint Redox‐Immune Homeostasis in Rheumatoid Arthritis

Advanced Science, EarlyView.
Mesenchymal stem cell‐derived extracellular vesicles (EVs) coat ruthenium‐loaded metal‐organic frameworks (Ru@ZrMOF), creating a targeted therapeutic (Ru@ZrMOF/EVs). This platform scavenges ROS, generates oxygen, and polarizes macrophages from M1 to M2, alleviating inflammation, inhibiting pannus, promoting cartilage repair, and downregulating HIF‐1α ...
Xingheng Wang, Jianbo Huang, Shuwei Zhang, Sujiao Cao, Fangxue Du, Liqiang Zhou, Li Qiu, Yuanjiao Tang   +7 more
wiley   +1 more source

Structural and Functional Characterization of EXPO‐Derived Extracellular Vesicles in Plants

Advanced Science, EarlyView.
In this study, 3D electron tomography (ET), cryo‐ET, and immunogold transmission electron microscopy (TEM) are employed to characterize plant extracellular vesicles (EVs) under physiological conditions. EVs are classified into three distinct categories according to their size, content, and molecular‐marker profiles. Furthermore, Exo70E2‐positive medium
Jiayang Gao, Yanbin Li, Shengqi Zhang, Yilin He, Zhiqi Liu, Juan Wang, Jingmin Hua, Jinyu Chen, Junru Zhong, Huan Zhong, Yiji Xia, Yong Cui, Liwen Jiang   +12 more
wiley   +1 more source

Mass photometry as a robust method for characterizing adeno-associated virus critical quality attributes in gene therapy vector

Molecular Therapy: Nucleic Acids
The use of adeno-associated viruses (AAVs) as gene therapy vectors is growing, resulting in a pressing need for efficient, reliable AAV analysis methods.
Guangyu Wang, Lei Yu, Xi Qin, Zexin Zhou, Yong Zhou, Chenggang Liang   +5 more
doaj   +1 more source

NDST3‐Induced Epigenetic Reprogramming Reverses Neurodegeneration in Parkinson's Disease

Advanced Science, EarlyView.
NDST3‐mediated epigenetic reprogramming revitalizes neuronal circuits in the substantia nigra and striatum to halt dopaminergic neuron degeneration and restore motor function in Parkinson's disease models. This strategy promotes neuronal maintenance and functional recovery, highlighting NDST3's therapeutic potential in neurodegenerative disorders ...
Yujung Chang, Yongwoo Na, Hyeonjoo Im, Garam Yang, Seungseon Yang, Hyun Soo Shim, Chunggoo Kim, GyeungYun Kim, Hyeok Ju Park, Hee Young Kim, Seung Eun Lee, Wonwoong Lee, Yoon Ha, Sungho Park, Jieun Kim, Won‐Young Cho, Woong Sun, Jong‐Seo Kim, Junsang Yoo   +18 more
wiley   +1 more source