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Seroprevalence of Anaplasma marginale in Texas Cattle
Preventive Veterinary Medicine, 2014To our knowledge the seroprevalence of Anaplasma marginale in Texas has not been reported. The objective of this study was to estimate the point seroprevalence and spatial distribution of Texas cattle persistently infected with A. marginale. This was a cross-sectional observational study examining serum collected from 12,000 adult cattle marketed in 23
Thomas B. Hairgrove +4 more
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Isolate of Anaplasma marginale not transmitted by ticks
American Journal of Veterinary Research, 1986SUMMARY The tick-borne transmissibility of 2 isolates of Anaplasma marginale was compared. Dermacentor variabilis were exposed to A marginale as nymphs by feeding on 1 of 4 splenectomized calves during periods of ascending parasitemia (maximum 49% to 81% parasitized erythrocytes) induced by injection of a stabilate.
R D, Smith +6 more
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A Study of the Immunological Relationship of Anaplasma Marginale and Anaplasma Centrale
Research in Veterinary Science, 1967SUMMARY A total of 112 experimental cases of anaplasmosis using Anaplasma marginale (from 3 sources) and Anaplasma centrale, in splenectomized calves, non-splenectomized calves, yearlings and adult cattle, have been studied to measure the immunological relationship of these anaplasms.
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Gram-Staining Characteristics of Anaplasma marginale
American Journal of Veterinary Research, 1973SUMMARY Purified erythrocyte-free suspensions of Anaplasma marginale were stained by Gram's method, Giemsa stain, acridine orange (ao) stain, and fluorescent antibody (fa) stain. Anaplasma marginale was observed to be gram-negative. Morphologic characteristics that were not discernible by the other methods were not detected by Gram's method.
T E, Amerault, V, Mazzola, T O, Roby
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Finite Purification of Anaplasma marginale: Serologic Inactivity of the Anaplasma Body
American Journal of Veterinary Research, 1977SUMMARY Anaplasma bodies finitely purified by affinity chromatography to eliminate all traces of erythrocytic stromata were not agglutinated by serum from calves having acute anaplasmosis. The stromata which were associated with the anaplasma bodies were also finitely purified and agglutinated with such serum.
J R, Budden, G T, Dimopoullos
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American Journal of Veterinary Research, 2008
Abstract Objective—To determine whether sequelae of infection differed among single versus double infection with Anaplasma phagocytophilum or Anaplasma marginale, with and without tick salivary extract, in cattle. Animals—Eighteen 13-month old steers. Procedures—Treatment groups of 3 cattle each included A marginale inoculated ID followed on day 35 by ...
Bruce R, Hoar +3 more
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Abstract Objective—To determine whether sequelae of infection differed among single versus double infection with Anaplasma phagocytophilum or Anaplasma marginale, with and without tick salivary extract, in cattle. Animals—Eighteen 13-month old steers. Procedures—Treatment groups of 3 cattle each included A marginale inoculated ID followed on day 35 by ...
Bruce R, Hoar +3 more
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Veterinary Microbiology, 1991
An Anaplasma centrale genomic library was constructed in pUC13. Two clones pAC5 and pAC137 hybridising to A. centrale and A. marginale DNA were isolated from this library. One of these, pAC5, also hybridised to DNA from A. ovis. The total insert of pAC5 was subcloned into pBR322. This subclone, pAC5-12, could detect 1 ng A.
E S, Visser, R E, Ambrosio, D T, de Waal
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An Anaplasma centrale genomic library was constructed in pUC13. Two clones pAC5 and pAC137 hybridising to A. centrale and A. marginale DNA were isolated from this library. One of these, pAC5, also hybridised to DNA from A. ovis. The total insert of pAC5 was subcloned into pBR322. This subclone, pAC5-12, could detect 1 ng A.
E S, Visser, R E, Ambrosio, D T, de Waal
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Development of a recombinant Anaplasma marginale DNA probe
Veterinary Microbiology, 1990An Anaplasma marginale DNA probe has been developed by using an improved method for the isolation of genomic DNA. Purified genomic A. marginale DNA from the St. Croix isolate was partially digested with Sau 3A1 into fragments (greater than or equal to 5.0 kb). The restriction fragments were cloned using standard techniques in the pBR322 vector and used
R, Aboytes-Torres, G M, Buening
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Evaluation of jackrabbits as nonruminant hosts for Anaplasma marginale
American Journal of Veterinary Research, 1985SUMMARY Two black-tailed jackrabbits (Lepus californicus), 1 splenectomized and 1 intact, were inoculated with 0.2 ml of a 1:5 dilution of a Florida Anaplasma marginale stabilate. Five months later, both hares were inoculated with 1 ml of whole blood from a calf with acute anaplasmosis. Neither hare developed any signs of clinical anaplasmosis.
J L, Zaugg, B A, Newman
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Ultrastructure of Anaplasma marginale After Freeze-Fracture
American Journal of Veterinary Research, 1975SUMMARY Stained thin sections and freeze-fractured replicas of Anaplasma marginale-infected bovine erythrocytes were examined by electron microscopy. Freeze-fracture replication not only verified basic Anaplasma ultrastructure, but also allowed visualization of structures not previously reported. Because of the partial 3-dimensional views obtained with
T E, Amerault, T O, Roby, R L, Sealock
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