Results 141 to 150 of about 2,869 (182)
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Gram-Staining Characteristics of Anaplasma marginale
American Journal of Veterinary Research, 1973SUMMARY Purified erythrocyte-free suspensions of Anaplasma marginale were stained by Gram's method, Giemsa stain, acridine orange (ao) stain, and fluorescent antibody (fa) stain. Anaplasma marginale was observed to be gram-negative. Morphologic characteristics that were not discernible by the other methods were not detected by Gram's method.
T E, Amerault, V, Mazzola, T O, Roby
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Finite Purification of Anaplasma marginale: Serologic Inactivity of the Anaplasma Body
American Journal of Veterinary Research, 1977SUMMARY Anaplasma bodies finitely purified by affinity chromatography to eliminate all traces of erythrocytic stromata were not agglutinated by serum from calves having acute anaplasmosis. The stromata which were associated with the anaplasma bodies were also finitely purified and agglutinated with such serum.
J R, Budden, G T, Dimopoullos
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American Journal of Veterinary Research, 2008
Abstract Objective—To determine whether sequelae of infection differed among single versus double infection with Anaplasma phagocytophilum or Anaplasma marginale, with and without tick salivary extract, in cattle. Animals—Eighteen 13-month old steers. Procedures—Treatment groups of 3 cattle each included A marginale inoculated ID followed on day 35 by ...
Bruce R, Hoar +3 more
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Abstract Objective—To determine whether sequelae of infection differed among single versus double infection with Anaplasma phagocytophilum or Anaplasma marginale, with and without tick salivary extract, in cattle. Animals—Eighteen 13-month old steers. Procedures—Treatment groups of 3 cattle each included A marginale inoculated ID followed on day 35 by ...
Bruce R, Hoar +3 more
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Veterinary Microbiology, 1991
An Anaplasma centrale genomic library was constructed in pUC13. Two clones pAC5 and pAC137 hybridising to A. centrale and A. marginale DNA were isolated from this library. One of these, pAC5, also hybridised to DNA from A. ovis. The total insert of pAC5 was subcloned into pBR322. This subclone, pAC5-12, could detect 1 ng A.
E S, Visser, R E, Ambrosio, D T, de Waal
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An Anaplasma centrale genomic library was constructed in pUC13. Two clones pAC5 and pAC137 hybridising to A. centrale and A. marginale DNA were isolated from this library. One of these, pAC5, also hybridised to DNA from A. ovis. The total insert of pAC5 was subcloned into pBR322. This subclone, pAC5-12, could detect 1 ng A.
E S, Visser, R E, Ambrosio, D T, de Waal
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Development of a recombinant Anaplasma marginale DNA probe
Veterinary Microbiology, 1990An Anaplasma marginale DNA probe has been developed by using an improved method for the isolation of genomic DNA. Purified genomic A. marginale DNA from the St. Croix isolate was partially digested with Sau 3A1 into fragments (greater than or equal to 5.0 kb). The restriction fragments were cloned using standard techniques in the pBR322 vector and used
R, Aboytes-Torres, G M, Buening
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Evaluation of jackrabbits as nonruminant hosts for Anaplasma marginale
American Journal of Veterinary Research, 1985SUMMARY Two black-tailed jackrabbits (Lepus californicus), 1 splenectomized and 1 intact, were inoculated with 0.2 ml of a 1:5 dilution of a Florida Anaplasma marginale stabilate. Five months later, both hares were inoculated with 1 ml of whole blood from a calf with acute anaplasmosis. Neither hare developed any signs of clinical anaplasmosis.
J L, Zaugg, B A, Newman
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Ultrastructure of Anaplasma marginale After Freeze-Fracture
American Journal of Veterinary Research, 1975SUMMARY Stained thin sections and freeze-fractured replicas of Anaplasma marginale-infected bovine erythrocytes were examined by electron microscopy. Freeze-fracture replication not only verified basic Anaplasma ultrastructure, but also allowed visualization of structures not previously reported. Because of the partial 3-dimensional views obtained with
T E, Amerault, T O, Roby, R L, Sealock
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Kinetics of the Antibody Response to Anaplasma Marginale Infection
Journal of Infectious Diseases, 1966A pattern of sequential synthesis of particular molecular species of immunoglobulins has been described in the response of several animal species to nonviable antigens such as proteins, polysaccharides, and inactivated bacteria. With few exceptions early, exclusive 7M globulin antibody synthesis is augmented or replaced by 7G antibody after periods ...
F A, Murphy, J W, Osebold, O, Aalund
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Recrudescence of Anaplasma marginale Induced by Immunosuppression with Cyclophosphamide
American Journal of Veterinary Research, 1981SUMMARY Eight doses of cyclophosphamide (5 mg/kg), at 2-day intervals between doses, were administered iv to Anaplasma-carrier splenectomized calves. Significant decreases in serum immunoglobulins, diminished complement-fixing antibody response, and transitory leukopenia were associated with the treatment.
D E, Corrier, G G, Wagner, L G, Adams
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Veterinary Immunology and Immunopathology, 1991
High titered antibody from rabbits immunized with Anaplasma centrale or from cattle recovered from A. centrale infection bound predominantly to several 33-36 kDa polypeptides present in both A. centrale and the Israel-NT isolate of Anaplasma marginale. High titered bovine antibody against the Israel-NT isolate of A. marginale also reacted predominantly
V, Shkap +3 more
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High titered antibody from rabbits immunized with Anaplasma centrale or from cattle recovered from A. centrale infection bound predominantly to several 33-36 kDa polypeptides present in both A. centrale and the Israel-NT isolate of Anaplasma marginale. High titered bovine antibody against the Israel-NT isolate of A. marginale also reacted predominantly
V, Shkap +3 more
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