Results 181 to 190 of about 7,966 (226)
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Anaplasma marginale in tick cell culture
American Journal of Veterinary Research, 1989SUMMARY Anaplasma marginale was propagated in a tick cell line derived from Dermacentor variabilis embryos. The rickettsial organism was identified and monitored in culture by transmission electron microscopy and the indirect immunofluorescence technique, using specific monoclonal antibodies.
R J, Hidalgo +3 more
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Differential extraction of antigens of Anaplasma marginale
American Journal of Veterinary Research, 1988SUMMARY Strain-, group-, and genus-specific antigens of the Florida isolate of Anaplasma marginale had different solubilities in zwitterionic, nonionic, and anionic detergents. On the basis of their solubility in nonionic detergent, antigens were grouped into 3 classes: (i) a 108-kilodalton (kD) group-specific antigen and a series of poorly defined ...
J H, Adams, R D, Smith
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A Study of the Immunological Relationship of Anaplasma Marginale and Anaplasma Centrale
Research in Veterinary Science, 1967SUMMARY A total of 112 experimental cases of anaplasmosis using Anaplasma marginale (from 3 sources) and Anaplasma centrale, in splenectomized calves, non-splenectomized calves, yearlings and adult cattle, have been studied to measure the immunological relationship of these anaplasms.
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Finite Purification of Anaplasma marginale: Serologic Inactivity of the Anaplasma Body
American Journal of Veterinary Research, 1977SUMMARY Anaplasma bodies finitely purified by affinity chromatography to eliminate all traces of erythrocytic stromata were not agglutinated by serum from calves having acute anaplasmosis. The stromata which were associated with the anaplasma bodies were also finitely purified and agglutinated with such serum.
J R, Budden, G T, Dimopoullos
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American Journal of Veterinary Research, 2008
Abstract Objective—To determine whether sequelae of infection differed among single versus double infection with Anaplasma phagocytophilum or Anaplasma marginale, with and without tick salivary extract, in cattle. Animals—Eighteen 13-month old steers. Procedures—Treatment groups of 3 cattle each included A marginale inoculated ID followed on day 35 by ...
Bruce R, Hoar +3 more
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Abstract Objective—To determine whether sequelae of infection differed among single versus double infection with Anaplasma phagocytophilum or Anaplasma marginale, with and without tick salivary extract, in cattle. Animals—Eighteen 13-month old steers. Procedures—Treatment groups of 3 cattle each included A marginale inoculated ID followed on day 35 by ...
Bruce R, Hoar +3 more
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Ultrastructure of Anaplasma marginale After Freeze-Fracture
American Journal of Veterinary Research, 1975SUMMARY Stained thin sections and freeze-fractured replicas of Anaplasma marginale-infected bovine erythrocytes were examined by electron microscopy. Freeze-fracture replication not only verified basic Anaplasma ultrastructure, but also allowed visualization of structures not previously reported. Because of the partial 3-dimensional views obtained with
T E, Amerault, T O, Roby, R L, Sealock
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Veterinary Microbiology, 1991
An Anaplasma centrale genomic library was constructed in pUC13. Two clones pAC5 and pAC137 hybridising to A. centrale and A. marginale DNA were isolated from this library. One of these, pAC5, also hybridised to DNA from A. ovis. The total insert of pAC5 was subcloned into pBR322. This subclone, pAC5-12, could detect 1 ng A.
E S, Visser, R E, Ambrosio, D T, de Waal
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An Anaplasma centrale genomic library was constructed in pUC13. Two clones pAC5 and pAC137 hybridising to A. centrale and A. marginale DNA were isolated from this library. One of these, pAC5, also hybridised to DNA from A. ovis. The total insert of pAC5 was subcloned into pBR322. This subclone, pAC5-12, could detect 1 ng A.
E S, Visser, R E, Ambrosio, D T, de Waal
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Kinetics of the Antibody Response to Anaplasma Marginale Infection
Journal of Infectious Diseases, 1966A pattern of sequential synthesis of particular molecular species of immunoglobulins has been described in the response of several animal species to nonviable antigens such as proteins, polysaccharides, and inactivated bacteria. With few exceptions early, exclusive 7M globulin antibody synthesis is augmented or replaced by 7G antibody after periods ...
F A, Murphy, J W, Osebold, O, Aalund
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Recrudescence of Anaplasma marginale Induced by Immunosuppression with Cyclophosphamide
American Journal of Veterinary Research, 1981SUMMARY Eight doses of cyclophosphamide (5 mg/kg), at 2-day intervals between doses, were administered iv to Anaplasma-carrier splenectomized calves. Significant decreases in serum immunoglobulins, diminished complement-fixing antibody response, and transitory leukopenia were associated with the treatment.
D E, Corrier, G G, Wagner, L G, Adams
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Development of a recombinant Anaplasma marginale DNA probe
Veterinary Microbiology, 1990An Anaplasma marginale DNA probe has been developed by using an improved method for the isolation of genomic DNA. Purified genomic A. marginale DNA from the St. Croix isolate was partially digested with Sau 3A1 into fragments (greater than or equal to 5.0 kb). The restriction fragments were cloned using standard techniques in the pBR322 vector and used
R, Aboytes-Torres, G M, Buening
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