Results 51 to 60 of about 9,009 (257)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Angle-of-arrival estimation in presence of multipath

Electronics Letters, 1991
The Thomson method of multiple-windows is applied to the problem of angle-of-arrival (AOA) estimation at low grazing angles, and in the presence of combined specular and diffuse multipath. Experimental data are used, collected by means of a 32 element sampled aperture antenna system operating at X band.
A. Drosopoulos, S. Haykin
openaire   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Performance Evaluation of Deep Learning Approaches for Angle Estimation Based on AoA and DoA Estimation

Applied Sciences
Accurate Angle of Arrival (AoA) and Direction of Arrival (DoA) estimation is crucial for Indoor Positioning Systems (IPS). Traditional signal processing methods, such as MUSIC and ESPRIT, provide high-resolution estimation at the cost of high ...
Seoyoung Koh, Jaeho Lee
doaj   +1 more source

Performance Analysis of Interferometer Algorithm under Phase Measurement Error

Applied Sciences, 2021
Direction finding has been extensively studied over the past decades and a number of algorithms have been developed. In direction finding, theoretic performance prediction is a fundamental problem.
Chan-Bin Ko, Joon-Ho Lee
doaj   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

A Method of Arrival Angle Optimization in Single-Station Positioning Based on Statistical Features

Remote Sensing
Aiming to mitigate the substantial dispersion in arrival angle estimation due to colored and white noise interference, which may seriously affect the accuracy of short-wave single-station positioning, this paper introduces an approach to optimizing ...
Ting Li, Tongxin Liu, Xuehai Yang, Guobin Yang, Chunhua Jiang, Chongzhe Lao   +5 more
doaj   +1 more source

Spatial Differencing Reconstruction Based Low-Angle DOA Estimation with MIMO Radar [PDF]

Xibei Gongye Daxue Xuebao, 2018
Due to the multipath effect, the direction of arrival (DOA) estimation performance for low-angle targets decreases greatly. To overcome this problem, this paper proposes a spatial differencing reconstruction based DOA estimation algorithm by using the ...

doaj   +1 more source

Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank

FEBS Open Bio, EarlyView.
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer, Pawel Rubach, Zbigniew Dauter, Wojciech Dec, Dariusz Brzezinski, Marta Kulik, Wladek Minor, Mariusz Jaskolski   +7 more
wiley   +1 more source

Using fluorescence fluctuation spectroscopy to quantify molecular dynamics within cells

FEBS Open Bio, EarlyView.
Microscopy techniques like fluorescence correlation spectroscopy (FCS) and number and brightness analysis (N&B) allow to quantify concentrations, diffusion rates, and oligomerization of fluorescently labeled molecules in the living cell. In this protocol the principles of how to set up these types of experiments are described including calibration ...
Mark A. Hink
wiley   +1 more source