Results 51 to 60 of about 166,061,951 (269)

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

A Method of Arrival Angle Optimization in Single-Station Positioning Based on Statistical Features

Remote Sensing
Aiming to mitigate the substantial dispersion in arrival angle estimation due to colored and white noise interference, which may seriously affect the accuracy of short-wave single-station positioning, this paper introduces an approach to optimizing ...
Ting Li, Tongxin Liu, Xuehai Yang, Guobin Yang, Chunhua Jiang, Chongzhe Lao   +5 more
doaj   +1 more source

Spatial Differencing Reconstruction Based Low-Angle DOA Estimation with MIMO Radar [PDF]

Xibei Gongye Daxue Xuebao, 2018
Due to the multipath effect, the direction of arrival (DOA) estimation performance for low-angle targets decreases greatly. To overcome this problem, this paper proposes a spatial differencing reconstruction based DOA estimation algorithm by using the ...

doaj   +1 more source

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

FEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong, Yuhei Hosokawa, Lars Oliver Essen, Manuel Maestre‐Reyna   +3 more
wiley   +1 more source

Angle-of-arrival estimation in presence of multipath

Electronics Letters, 1991
The Thomson method of multiple-windows is applied to the problem of angle-of-arrival (AOA) estimation at low grazing angles, and in the presence of combined specular and diffuse multipath. Experimental data are used, collected by means of a 32 element sampled aperture antenna system operating at X band.
A. Drosopoulos, S. Haykin
openaire   +1 more source

Application of Kalman Filtering Approach on Signal Angle-of-Arrival Estimates

2023 31st Signal Processing and Communications Applications Conference (SIU), 2023
This study focuses on modifying the Kalman Filtering (KF) approach, whose measurement noise suppression capability had been proven many times, especially in multidimensional positioning problems, to serve one-dimensional signal angle-of-arrival (AoA) measurements, and examining the overall AoA estimation performance.
Yildiz, Umut, Coskun, Ahmet Faruk
openaire   +2 more sources

Optimizing photoexcitation conditions for time‐resolved X‐ray solution scattering experiments

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering (TR‐XSS) is a powerful technique to visualize how proteins change their structure in real time after light activation. Selecting the right laser photoexcitation conditions—fluence, excitation geometry, and sample refresh rate—is critical to maximize the experimental signal while avoiding unwanted side effects ...
Matteo Levantino
wiley   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source