Results 251 to 260 of about 4,974,799 (302)
FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
FEBS Open Bio, EarlyView.The brown marmorated stink bug, Halyomorpha halys, mainly secretes 4‐oxo‐(E)‐2‐hexenal, (E)‐2‐decenal, dodecane and tridecane, a mixture representative of that released by many heteropterans. We demonstrated that H. halys can synthesise these secretory components de novo using dietary glucose.
Haruna Fujimori, Koji Noge
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Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus
FEBS Open Bio, EarlyView.BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima +7 more
wiley +1 more source
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Experiments with computer animation
Proceedings of the 3rd annual conference on Computer graphics and interactive techniques, 1976Three generally accepted facts are:1. Computers are getting cheap and fast enough to do cost-effective graphics on line and in real time;2. The future of computer graphics is in raster scan;3. Computer animation is emerging as a volatile art form and as a medium for scientific communication.Concurrently, we find computer animation somewhat polluted and
Nicholas Negroponte, Paul Pangaro
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2015
Towards the end of his autobiography, Mohandas K. Gandhi declares that: ‘the more helpless a creature, the more entitled it is to protection by man from the cruelty of man’ (1993: 235). For Gandhi, the practice of ahimsa (non-violence) at the heart of his ethico-politics sought to reach well beyond the limits of the human.
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Towards the end of his autobiography, Mohandas K. Gandhi declares that: ‘the more helpless a creature, the more entitled it is to protection by man from the cruelty of man’ (1993: 235). For Gandhi, the practice of ahimsa (non-violence) at the heart of his ethico-politics sought to reach well beyond the limits of the human.
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The ethics of experiments on higher animals
Social Science & Medicine. Part F: Medical and Social Ethics, 1981It has been demonstrated over the last decade that some higher animals can learn sign language and communicate with humans. This finding radically alters some earlier conceptions of animals as being non-sentient, and forces a re-evaluation of the use of higher animals in research.
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Animal Experiments and Animal Rights
2012This chapter explores grass-roots activism as it is associated with other animals in society. Grass-roots activism works at the level of subpolitics (Beck, 1992) rather than at the level of political parties. Grassroots groups, such as Greenpeace, the National Anti-Vivisection Society (NAVS) and People for the Ethical Treatment of Animals (PETA ...
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Experimental Animals and Animal Experiments
1988In the last chapter the importance of animal experimentation for the current production and control of vaccines has already been referred to several times without discussing its substance. This subject will be elaborated in the following sections with special attention devoted to the extent, purpose and relevance of this research and the pain or ...
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