Results 101 to 110 of about 2,201,882 (299)

Adsorption of plant phenols by polystyrene resins

Acta Societatis Botanicorum Poloniae, 2014
Adsorption of nine plant phenols by nine polystyrene ion-exchange resins was investigated in an experimental model system. The phenols were adsorbed by Amberlite CG-120 more efficiently than any other acidically charged resins tested in this study.
Akiyoshi Fukushima, Hioaki Hase, Koshi Saito   +2 more
doaj   +1 more source

Cryo‐EM Analysis of Adenovirus Vector Reveals Purification Conditions Leading to Loss of Viral Fiber Proteins and Reduced Infectivity

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Vaccines and gene therapies require efficient delivery of genetic material to host cells. Viral vectors leverage viruses' ability to enter host cells to achieve this. Following the SARS‐CoV‐2 vaccine and novel gene therapies that utilize adenovirus vectors, there has been an increase in the demand for large‐scale production of vectors.
Betsy Widdicombe, Joseph D. Barritt, Nichakorn Pipatpadungsin, Braulio Carrillo Sanchez, Dale Stibbs, Lisa Cooper, Sarah L. Rouse, Daniel G. Bracewell   +7 more
wiley   +1 more source

Evaluation of anion exchange resins for pre-analysis separation of actinides

, 1977
Anion exchange is the technique usually employed to separate plutonium and neptunium from actinides and other sample constituents prior to radiochemical determinations of the actinides.
Navratil, J. D.
core  

Nuclease‐Free Scalable Purification of Physalis Mottle Virus‐Like Particles Expressed in Escherichia coli

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Plant virus‐like particles (VLPs) are promising nanotechnology platforms, but their translation requires purification strategies suitable for large‐scale production. Here, we developed a nuclease‐free downstream process for Physalis mottle virus (PhMV) VLPs expressed in Escherichia coli, combining ammonium sulfate precipitation, low‐pH ...
Sean K. Hsu, Patrick Opdensteinen, Tariq Hussain, Nicole F. Steinmetz   +3 more
wiley   +1 more source

Feasibility Assessment of a Single‐pH Connected Polishing Train for Monoclonal Antibody Purification Using Mildly Acidic Protein A Elution and an Acidic‐Window Anion Exchange Membrane

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Monoclonal antibody downstream processing often requires multiple pH adjustments between Protein A capture and polishing steps. These adjustments increase process complexity and make direct unit‐operation connection more difficult. We evaluated a connected post‐capture polishing block operated at approximately pH 5.0 using mild‐pH Protein A ...
Takaki Aiso, Hiroshi Muto, Kyota Suzuki, Yumiko Masuda, Hirofumi Kakihara, Masashi Sonoyama   +5 more
wiley   +1 more source

Preparation and in Vitro Hypoglycemic Activity Analysis of Selenoexopolysaccharides from Weizmannia coagulans P-L1

Shipin gongye ke-ji
To develop a method for the preparation of selenium exopolysaccharide (Se-EPS) with high yield, the heat-resistant Weizmannia coagulans P-L1 was selected to produce Se-EPS through fermentation, with the addition of sodium selenite at a concentration of ...
Xinlong XIANG, Qianhui GE, Zhiyu ZHANG, Bo WU, Ying ZHANG, Haibin WANG, Lijuan PENG   +6 more
doaj   +1 more source

Adsorption of Monobutyl Phthalate from Aqueous Phase onto Two Macroporous Anion-Exchange Resins

Journal of Chemistry, 2014
As new emerging pollutants, phthalic acid monoesters (PAMs) pose potential ecological and human health risks. In the present study, adsorption performance of monobutyl phthalate (MBP) onto two macroporous base anion-exchange resins (D-201 and D-301) was ...
Zhengwen Xu, Yunlong Zhao, Jing Shi, Jiangang Lu, Ling Cheng, Mindong Chen   +5 more
doaj   +1 more source

Serotype‐Tailored Midstream and Downstream Operations to Advance the Manufacturing of Adeno‐Associated Viral Vectors

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Adeno‐associated viruses (AAVs) are the leading vectors for in vivo gene therapy, fueling a growing demand for scalable and robust biomanufacturing processes. Yet, the impact of cell lysis conditions on the performance of midstream and downstream unit operations across serotypes remains poorly characterized.
Arianna Minzoni, Shriarjun Shastry, Eduardo Barbieri, Morgan R. Hurst, Kelvin Idanwekhai, Yuxuan Wu, Katherine Ernst, Wai‐Keen Chung, Christopher Bombeck, Saad A. Khan, Sushmita Koley, Gabriella Kiss, Oliver Rammo, Alexander Tropsha, Cristiana Boi, Michael A. Daniele, Steven Cramer, Barry Byrne, Stephen Kaminsky, Angie Snell Bennett, Stefano Menegatti   +20 more
wiley   +1 more source

Pb₃F₅NO₃, a Cationic Layered Material for Anion-Exchange

, 2016
Our research involves the development of new cationic materials for anion-based applications. We report the solvothermal synthesis and characterization of Pb3F5NO3, a new layered lead fluoride material that, unlike the majority of layered and open ...
Peter Y. Zavalij (616937), Scott R. J. Oliver (1406707), Dat T. Tran (1543018)   +2 more
core   +1 more source

Continuous Capture of Recombinant Adeno‐Associated Virus Particles Using Twin‐Column CaptureSMB

Biotechnology and Bioengineering, EarlyView.
Continuous twin‐column CaptureSMB enables direct capture of secreted rAAV5 from minimally processed perfusion harvest, bypassing lysis, clarification, and concentration steps. Compared to batch capture, it achieves higher yield (+14.3%), greater productivity (+11.4%), and drastically reduced buffer use (−79.2%), while maintaining critical quality ...
Julia M. Müller, Daniela Tobler, Jules Bühler, Damian Hauri, Richard Plieninger, Sven Göbel, Efe Saygili, Yuki Higuchi, Ryosuke Takahashi, Sebastian Vogg, Thomas Müller‐Späth, Thomas K. Villiger   +11 more
wiley   +1 more source