Results 301 to 310 of about 570,434 (333)
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Plaque Formation in Agar by Single Antibody-Producing Cells
Science, 1963Distinct plaques, each of which is due to the release of hemolysin by a single antibody-forming cell, are revealed by complement after incubation, in an agar layer, of a mixture of sheep red cells and lymphoid cells from a rabbit immunized with sheep red cells.
N K, Jerne, A A, Nordin
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Antibody-producing cells in human periapical granulomas and cysts
Journal of Endodontics, 1981Immunoglobulin-producing cells were quantitated in 20 human periapical granulomas and cysts. Results showed that 42% of the lymphoid cells comprising 19% of all inflammatory cells contained immunoglobulins. The majority of lymphocytes (81%) were not associated with immunoglobulin production. IgG was found in 74%, IgA in 20%, IgE in 4%, and IgM in 2% of
M H, Stern +3 more
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Human:Mouse Antibody-Producing Transfectoma Cell Lines
1985A new means to generate antibodies (Ab’s) with desired antigen-specificities and biological effector functions is to create transfectoma Ab-producing cell lines. These cell lines are derived from lymphoid cell lines that have been transfected with novel immunoglobulin genes using DNA-mediated gene transfer techniques.
Vernon T. Oi, Sherie L. Morrison
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Immunocytochemical localization of antibody-producing cells in adult lamprey
Immunology Letters, 1983Antibody-producing cells were localized in the fat column of adult lamprey previously immunized with 'O'RBC. These cells were visualized using rabbit anti-lamprey antibody antiserum in the peroxidase anti-peroxidase (PAP) immunocytochemical technique.
M, Hagen, M F, Filosa, J H, Youson
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Separation of mitogen-induced suppressor cells of human antibody-producing cells
Cellular Immunology, 1980Abstract Human antibody-forming cells were demonstrated by a plaque in agar technique following in vitro stimulation with either pokeweed mitogen or Cowan I strain of protein A-positive Staphylococcus aureus bacteria. We evaluated the effects on this antibody formation caused by the addition of cells which had been stimulated with PH A or Con A. Both
J T, Kurnick, F, Pandolfi, H, Wigzell
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Studies on antibody-producing cells. I. Ultrastructure of 19S and 7S antibody-producing cells.
The Journal of experimental medicine, 1972Antibody-bearing cells of spleen and lymph node of the mouse and rabbit detected by rosette formation with the antigenic red blood cells were collected by micropipet and studied by electron microscopy. More than 300 such cells were examined. In the lymph nodes, rosette-forming cells were all in the lymphocytic and plasmacytic categories.
Gudat, F G +3 more
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Toxin–antigen conjugates as selection tools for antibody producing cells
Journal of Immunological Methods, 2013The generation of antibodies with designated specificity requires cost-intensive and time-consuming screening procedures. Here we present a new method by which hybridoma cells can be selected based on the specificity of the produced antibody by the use of antigen-toxin-conjugates thus eliminating the need of a screening procedure.
Messerschmidt, Katrin, Heilmann, Katja
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SERUM FREE CRYOPRESERVATION OF ANTIBODY PRODUCING CELLS
1992ABSTRACT The use of Foetal Bovine Serum (FBS) in the culture of cells for the production of human therapeutics is undesirable. The risk of introducing mycoplasma, viruses, entities responsible for spongiform encephalopathy and other adventitious agents from FBS can be eliminated in culture by the use of serum free media.
J.A.J. Hanak +4 more
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Specific Antibacterial Antibody-Producing Cells in Human Nasal Mucosa
1987Recurrent infections of the rhinopharyngeal area, common in young children, are elusive to most classical therapies. The use of local vaccines to enhance local immunity has been proposed since the sixties. Although these immunomodulators have been widely used, their mechanisms of action remain disputed.
M C, Béné, G, Faure, M, Wayoff
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Growth of antibody-producing cell clones in microcultures
1987Publisher Summary This chapter describes growth of antibody-producing cell clones in microcultures. The objective of the microculture technique is to analyze the frequency of rare cells involved in the immune response. The precursors of antibody-forming cells (B cells), helper T cells, and suppressor T cells are considered rare cells.
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