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World Journal of Microbiology and Biotechnology, 2014
Alkaliphilic halotolerant cyanobacterium Aphanothece halophytica showed optimal growth in the medium containing 0.5 M NaCl. The increase of exogenously added glycine to the medium up to 10 mM significantly promoted cell growth under both normal (0.5 M NaCl) and salt stress (2.0 M NaCl) conditions.
Aporn, Bualuang, Aran, Incharoensakdi
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Alkaliphilic halotolerant cyanobacterium Aphanothece halophytica showed optimal growth in the medium containing 0.5 M NaCl. The increase of exogenously added glycine to the medium up to 10 mM significantly promoted cell growth under both normal (0.5 M NaCl) and salt stress (2.0 M NaCl) conditions.
Aporn, Bualuang, Aran, Incharoensakdi
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Current Microbiology, 2000
We have investigated conditions leading to the degradation of glycinebetaine in Aphanothece halophytica and have shown the activity of betaine-homocysteine methyltransferase (BHMT). The intracellular glycinebetaine level was decreased approximately 50% after 36 h salt downshock from 2.0 m NaCl medium to 0.5 m NaCl medium.
A, Incharoensakdi, R, Waditee
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We have investigated conditions leading to the degradation of glycinebetaine in Aphanothece halophytica and have shown the activity of betaine-homocysteine methyltransferase (BHMT). The intracellular glycinebetaine level was decreased approximately 50% after 36 h salt downshock from 2.0 m NaCl medium to 0.5 m NaCl medium.
A, Incharoensakdi, R, Waditee
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Probing of the betB gene from the cyanobacterium Aphanothece halophytica
The halotolerant cyanobacterium Aphanothece halophytica synthesizes the osmoprotectant glycine betaine in response to osmotic stress when the cells were grown in high salt medium. Choline is converted to betaine aldehyde, which in turn is converted further to glycine betaine. The enzymes involved are the products of betAB genes.openaire +1 more source
Characterization of Na+- atpase in the halotolerant cyanobacterium aphanothece halophytica
Extrusion of sodium ion is needed to balance and maintain sodium level for microorganisms to survive in hypersalin environment. Activity of Na+- ATPase in halotolerant cyanobacterium aphanothece haolphytica was investigated. Cells were preloaded with 22Na+ and the extrusion of 22Na+ from cells was followed by energizing the cells with glucose.openaire +1 more source
Uptake of cholene and its oxidation in halotolerant cynobacterium Aphanothece halophytica
Choline is a trim ethylated nitrogen compound, a precursor of glycine betaine which is one of the most potent osmoprotectants. Uptake of exogenous [methyl-14C] choline upon osmotic stress by a halotolerant cyanobacterium Aphcmothece halophytica was investigated.openaire +1 more source
Anais do Simpósio Estadual de Produtos Naturais, 2021
Laura Almeida Pinho +5 more
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Laura Almeida Pinho +5 more
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Halotolerant cyanobacterium Aphanothece halophytica grown under various NaCl concentrations from 0.2 – 3.0 M showed the optimum NaCl concentration for growth at 0.5 M NaCl. The salt stress condition that decreased growth to half of maximum growth rate was 2.0 M NaCl.
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This study on glycine uptake by alkaliphilic halotolerant cyanobacterium Aphanothece halophytica comprised two parts. The first part dealt with biochemical characterization of the uptake whereas the second part involved molecular characterization. Cells were grown under various concentrations of NaCl from 2.5-3.0 M.
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Nitrate is a major nitrogen source for the growth of cyanobacteria. Nitrate reductase (NR) catalyzes the conversion of nitrate to nitrite. The enzyme is important in nitrate metabolism regulation. In this work, we found that ammonium, the intermediate of nitrogen metabolism, reduced nitrate reductase in Aphanothece halophytica. Glutamine also decreased
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