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Expression of the melittin gene of Apis cerana cerana in Escherichia coli

Protein Expression and Purification, 2004
A cDNA encoding melittin in Apis cerana cerana was obtained by PCR from the recombinant plasmid and cloned into the GST fusion expression vector pGEX-4T-2 for expression of the protein. The expressed protein of about 29 kDa was detected by Western blot and triple antibody sandwich ELISA, indicating that the recombinant protein is the fusion protein of ...
Wan-jun, Shi   +3 more
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Activating transcription factor 2 (AccATF2) regulates tolerance to oxidative stress in Apis cerana cerana

Pesticide Biochemistry and Physiology, 2022
Activating transcription factor 2 (ATF2), a basic leucine zipper (bZIP) transcription factor, plays a crucial role in immune and DNA damage response in mammals. However, the function of ATF2 in insects remains unknown. Here, we isolated the ATF2 gene from Apis cerana cerana (AccATF2) and found that AccATF2 was a main regulator of the honeybee response ...
Lijun, Wang   +6 more
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Identification and functional characterization of AcerOBP15 from Apis cerana cerana (Hymenoptera: Apidae)

Apidologie, 2021
Coordination in honeybees is heavily dependent on their chemoreception system, in which odorant-binding proteins are very important. A previous study based on the antennal transcriptome of Apis cerana cerana revealed that OBP15 was significantly up-regulated at 25-day-old.
Yali Du   +4 more
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[Overwintering and size dynamic characters of Apis cerana cerana].

Ying yong sheng tai xue bao = The journal of applied ecology, 2003
A systematic observation and research was conducted on the overwintering and size dynamic characters of Apis cerana cerana at south and west mountaneous area of Anhui Province in 1998-2003. The results showed that the center temperature of overwintering bee flock changed synchronously with the outside temperature. With the decrease of overwintering bee
Linsheng, Yu, Shengming, Han
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Proteomic characterization of royal jelly proteins in Chinese (Apis cerana cerana) and European (Apis mellifera) honeybees

Biochemistry (Moscow), 2008
In this study, the proteins contained in royal jelly (RJ) derived from Chinese and European honeybees have been analyzed in detail and compared. Remarkable differences were found in the heterogeneity of major royal jelly proteins (MRJPs), MRJP2 and MRJP3, in terms of molecular weight and isoelectric points between the two species of RJ. MRJP2 and MRJP3
Qu, Ning   +5 more
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Apis cerana Fabricius 1793

Published as part of Lee, Dong-Yeol, Jeong, Ilyeong, Kim, Seonmin, Choi, Jae Won, Won, Min Hyeok, Kim, Donguk, Kim, Dongmin, Kim, Young-Kun, Jeon, Jiseung, Ryu, Jihun, Bang, WooJun, Chang, Jun Hyuk & Choi, Kwang Shik, 2024, Checklist for the insect fauna of two East Sea Islands (Ulleungdo Is. and Dokdo Is.) in the Republic of Korea, pp.
Lee, Dong-Yeol   +12 more
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Transcriptomic investigation of immune responses of the Apis cerana cerana larval gut infected by Ascosphaera apis

Journal of Invertebrate Pathology, 2019
Chalkbrood is the most common fungal disease in honeybees. The objective of this study was to reveal immune responses in the Apis cerana cerana larval gut following Ascosphaera apis invasion. Combining a previously assembled transcriptome of A. c. cerana larval gut and the high-throughput sequencing data obtained in this study, 6152 differentially ...
Rui, Guo   +5 more
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การเตรียมดีเอ็นเอติดตามเพื่อวิเคราะห์การแปรผันสายพันธุ์ผึ้งโพรง Apis cerana

ผึ้งโพรง (Apis cerana) เป็นพื้นเมืองชนิดหนึ่งที่พบในประเทศไทยและหลายๆ ประเทศในทวีปเอเชีย จากการศึกษาทางด้านสัณฐานวิทยาพบว่ามีความหลากหลายของสายพันธุ์แต่ยังไม่สามารถจำแนกเป็นสายพันธุ์ย่อยได้อย่างชัดเจน ในการวิจัยครั้งนี้ได้นำเทคนิคทางพันธุวิศวกรรมเข้ามาช่วยในการจัดจำแนกกลุ่มต่างๆ ที่แตกต่างกันในประเทศไทย โดยใช้เทคนิค Restriction Fragment Length ...
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Identification and characterization of two phospholipid hydroperoxide glutathione peroxidase genes from Apis cerana cerana

Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 2010
Phospholipid hydroperoxide glutathione peroxidase (PHGPX) plays a crucial role in maintaining the integrity of membrane by reducing hydroperoxides of phospholipids. Here, we report the identification and characterization of two genes, designated AccGtpx-1 and AccGtpx-2, encoding PHGPX proteins from the Chinese honeybees, Apis cerana cerana.
Mian, Wang   +3 more
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การผลิตและคุณภาพของรอยัลเยลลีจากผึ้งโพรง (Apis cerana)

การศึกษาปัจจัยและวิธีผลิตรอยัลเยลสีจากผึ้งโพรงไทย (A. cerana Indlca) พบว่า การใช้ถ้วยเพาะ ไขผึ้ง 12 ขนาดคือ 7x9, 7x10, 7x11, 8x9, 8x10, 8x11, 9x9, 9x10, 9x11, 10x9, 10x10 และ 10x11 ตารางมิลลิเมตร ได้ปริมาณรอยัลเยลสีรวมจากการทำอย่างละ 24 ถ้วยเท่ากับ 1.4, 1.4, 1.7, 1.7, 1.8, 1.6, 1.8, 2.0, 1.2, 1.7, 1.1 และ 0.4 กรัม ตามลำดับ การใช้ถ้วยเพาะขนาด 9x10 ...
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