Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Chimeric antigen receptor-engineered cell therapy for asthma: advances in targeting type 2 inflammation and translational challenges. [PDF]
Niu Y +6 more
europepmc +1 more source
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Effectiveness of Nurse-Led Digital Health Interventions on Symptom Management and Quality of Life in Cancer Patients Undergoing Systemic Therapy: A Systematic Review of Randomized Controlled Trials. [PDF]
Alqaisi O +5 more
europepmc +1 more source
Recent Advances in Fault Diagnosis and Opacity Analysis in Discrete Event Systems. [PDF]
Mangini AM +4 more
europepmc +1 more source
Design and Initial Evaluation of a Low-Cost Microprocessor-Controlled Ankle Prosthesis. [PDF]
Bigaliyeva Z +11 more
europepmc +1 more source
Prompt-Guided Semantic Latent Direction Learning in Diffusion Models for Abstract Visual Concept Manipulation. [PDF]
Khalid M +4 more
europepmc +1 more source
A nonlinear exaggerated kernel framework for expressive hair motion in tracking solution-based simulation. [PDF]
Park D, Kim JH.
europepmc +1 more source
Data-Driven Inverse Design of Silver Nanoparticle Size for Controlled Synthesis Across Multiple Systems Using Conditional Generative Models. [PDF]
Zi X +8 more
europepmc +1 more source

