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Metabolism of glycosylphosphatidylinositol-anchored proteins in Arabidopsis
Biochemical Society Transactions, 2000Although glycosylphosphatidylinositol (GPI)-anchored proteins have now been found in several plants, very little is known regarding their metabolism there. This report describes studies of the biosynthesis and turnover of arabinogalactan proteins, a class of abundant GPI-anchored proteins secreted by cultured Arabidopsis cells.
L, Darjania +5 more
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In Vitro Prenylation Assay of Arabidopsis Proteins
2013Protein prenylation, like other lipid posttranslational modifications of eukaryotic proteins, plays important roles in protein-membrane association and protein-protein interactions. In Arabidopsis, hundreds of proteins involved in a great variety of biological processes are potential prenylation substrates that need to be verified, including ...
Wan, Shi, Qin, Zeng, Mark P, Running
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G-protein-coupled signaling in Arabidopsis
Current Opinion in Plant Biology, 2002With an essentially complete plant genome in hand, it is now possible to conclude that a single or possibly just two canonical heterotrimeric G-protein complexes are present in Arabidopsis. In stark contrast, more than one hundred such complexes are found in some metazoans.
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The ‘protein complex proteome’ of chloroplasts in Arabidopsis thaliana
Journal of Proteomics, 2013Here, a first GelMap of the chloroplast "protein complex proteome" of Arabidopsis thaliana is presented. The GelMap software tool allows assigning multiple proteins to gel spots, thereby taking advantage of the high sensitivity of state-of-the-art mass spectrometry systems.
Christof, Behrens +5 more
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Telomere-binding proteins of Arabidopsis thaliana
Plant Molecular Biology, 1995The nucleoprotein structure of Arabidopsis thaliana telomeres was investigated. A protein specifically binding to telomeric sequences was characterized by gel mobility shift assays with synthetic oligonucleotides consisting of four 7 bp telomeric repeats of Arabidopsis (TTTAGGG) and crude nuclear protein extracts of Arabidopsis leaves.
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A Framework to Investigate Peroxisomal Protein Phosphorylation in Arabidopsis
Trends in Plant Science, 2019Peroxisomes perform essential roles in a range of cellular processes, highlighted by lipid metabolism, reactive species detoxification, and response to a variety of stimuli. The ability of peroxisomes to grow, divide, respond to changing cellular needs, interact with other organelles, and adjust their proteome as required, suggest that, like other ...
Amr R A, Kataya +2 more
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Immunofluorescence Microscopy for Localization of Arabidopsis Chloroplast Proteins
2011Immunofluorescence microscopy reveals localization of proteins in cells and tissues by means of highly specific, fluorescently labeled antibodies. This technique is an important complement to localization methods that use genetically encoded fluorescent tags. This chapter describes the five stages of immunofluorescence localization of proteins in plant
Stanislav, Vitha +1 more
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Studying Proteases and Protein Turnover in Arabidopsis Chloroplasts
2011Proteolysis is a key process for maintaining homeostasis in all living cells. The ability to degrade specific metabolic enzymes and regulatory proteins is essential for both cellular integrity and function. Equally important is the efficient removal of damaged or otherwise inactive polypeptides, especially during periods of developmental change or ...
Lars L E, Sjögren, Adrian K, Clarke
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Remorin proteins in Arabidopsis thaliana
2014Die Plasmamembran lebender Zellen stellt die Hauptbarriere für alle Arten von extrazellulären Signalen dar. Viele davon werden ins Innere der Zelle weitergeleitet, hier lösen sie im Kern transkiptionelle Veränderungen und damit die Anpassung der Zelle auf Proteinebene aus.
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Protein phosphorylation regulation in Arabidopsis
2018The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. ; Title from title screen of research.pdf file (viewed on July 18, 2008) ; Vita. ; Thesis (Ph. D.)
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