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Proteins are polyisoprenylated in Arabidopsis thaliana

Biochemical and Biophysical Research Communications, 2004
Isoprenoid lipids were found to be covalently linked to proteins of Arabidopsis thaliana. Their identity (polyprenols: Prenol-9-11 with Pren-10 dominating and dolichols: Dol-15-17 with Dol-16 dominating) was confirmed by means of HPLC/ESI-MS with application of the multiple reaction monitoring technique as well as metabolic labeling of Arabidopsis ...
Malgorzata, Gutkowska   +6 more
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Protein Coimmunoprecipitation in Arabidopsis

Cold Spring Harbor Protocols, 2007
INTRODUCTIONCoimmunoprecipitation is one of the most useful techniques for revealing protein-protein interactions. Briefly, it can be divided into three steps: (1) preparation of cell extracts without disruption of protein interactions; (2) formation of the antibody-antigen complexes; and (3) affinity purification of the protein complexes and ...
Giovanna, Serino, Xing-Wang, Deng
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F-box proteins in Arabidopsis

Trends in Plant Science, 2000
We thank Dietz Bauer and David Somers for critical reading of the manuscript. This work was supported by the MLS, OARDC and PMBB at The Ohio State University to J-C.J. Salaries and research support provided by state and federal funds appropriated to the Ohio Agricultural Research and Development Center, The Ohio State University.
W, Xiao, J, Jang
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Chromosomal proteins of Arabidopsis thaliana

Plant Molecular Biology, 1988
In plants with large genomes, each of the classes of the histones (H1, H2A, H2B, H3 and H4) are not unique polypeptides, but rather families of closely related proteins that are called histone variants. The small genome and preponderance of single-copy DNA in Arabidopsis thaliana has led us to ask if this plant has such families of histone variants. We
C P, Moehs, E F, McElwain, S, Spiker
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The Arabidopsis LHP1 protein is a component of euchromatin

Planta, 2005
The HP1 family proteins are involved in several aspects of chromatin function and regulation in Drosophila, mammals and the fission yeast. Here we investigate the localization of LHP1, the unique Arabidopsis thaliana HP1 homolog known at present time, to approach its function.
Libault, M.   +5 more
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The Arabidopsis ESCRT protein–protein interaction network

Plant Molecular Biology, 2011
In yeast, endosomal sorting of monoubiquitylated transmembrane proteins is performed by a subset of the 19 "class E vacuolar protein sorting" proteins. The core machinery consists of 11 proteins that are organised in three complexes termed ESCRT I-III (endosomal sorting complex required for transport I-III) and is conserved in eukaryotic cells.
Mojgan, Shahriari   +5 more
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Identification of proteins interacting with Arabidopsis ACD11

Journal of Plant Physiology, 2009
The Arabidopsis ACD11 gene encodes a sphingosine transfer protein and was identified by the accelerated cell death phenotype of the loss of function acd11 mutant, which exhibits heightened expression of genes involved in the disease resistance hypersensitive response (HR).
Petersen, Nikolaj H T   +6 more
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Identification of Feronia-interacting proteins in Arabidopsis thaliana

Genes & Genomics, 2022
Plant growth and development are complex processes modulated by numerous genes, transcription factors, hormones, and peptides. Several reports implicate the membrane-localized Catharanthus roseus receptor-like kinase1 (CrRLK1L) protein, FERONIA (FER), involved in plant development.
Jae-Han Choi, Ji-Woo Kim, Man-Ho Oh
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In Vitro Prenylation Assay of Arabidopsis Proteins

2013
Protein prenylation, like other lipid posttranslational modifications of eukaryotic proteins, plays important roles in protein-membrane association and protein-protein interactions. In Arabidopsis, hundreds of proteins involved in a great variety of biological processes are potential prenylation substrates that need to be verified, including ...
Wan, Shi, Qin, Zeng, Mark P, Running
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