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Gene targeting in Arabidopsis thaliana
Molecular and General Genetics MGG, 1992Gene targeting of a chromosomally integrated transgene in Arabidopsis thaliana is reported. A chimeric gene consisting of the promoter of the 35S RNA of CaMV, the polyadenylation signal of the octopine synthase gene and the coding region of the bacterial hygromycin phosphotransferase gene (hpt), which was rendered non-functional by deletion of 19 bp ...
Halfter, U., Morris, P., Willmitzer, L.
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Annual Review of Genetics, 1987
Arabidopsis thaliana is a small weed in the mustard family. It has been a convenient subject for studies in classical genetics for over forty years (35, 76). Recently, investigators have recognized that this flowering plant also has a genome size and genomic organization that recommend it for certain experiments in molecular genetics (41, 56, 57). As a
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Arabidopsis thaliana is a small weed in the mustard family. It has been a convenient subject for studies in classical genetics for over forty years (35, 76). Recently, investigators have recognized that this flowering plant also has a genome size and genomic organization that recommend it for certain experiments in molecular genetics (41, 56, 57). As a
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2021
Aims. Copper (Cu) is a micronutrient that has roles in photosynthesis, respiration, antioxidant activity, cell wall metabolism and hormone perception. Excess Cu is toxic and therefore its delivery has to be tightly regulated. Methods. Light and fluorescent microscopy, laser scanning confocal microscope. Results.
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Aims. Copper (Cu) is a micronutrient that has roles in photosynthesis, respiration, antioxidant activity, cell wall metabolism and hormone perception. Excess Cu is toxic and therefore its delivery has to be tightly regulated. Methods. Light and fluorescent microscopy, laser scanning confocal microscope. Results.
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Grafting with Arabidopsis thaliana
2016Generating chimeric organisms is an invaluable way to study cell-to-cell movement and non-cell-autonomous actions of molecules. Plant grafting is an ancient method of generating chimeric organisms and recently has been used to study the movement of hormones, proteins, and RNAs.
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In planta transformation of Arabidopsis thaliana
Molecular and General Genetics MGG, 1994Transformants of Arabidopsis thaliana can be generated without using tissue culture techniques by cutting primary and secondary inflorescence shoots at their bases and inoculating the wound sites with Agrobacterium tumefaciens suspensions. After three successive inoculations, treated plants are grown to maturity, harvested and the progeny screened for ...
Katavic, Vesna +4 more
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Proteins are polyisoprenylated in Arabidopsis thaliana
Biochemical and Biophysical Research Communications, 2004Isoprenoid lipids were found to be covalently linked to proteins of Arabidopsis thaliana. Their identity (polyprenols: Prenol-9-11 with Pren-10 dominating and dolichols: Dol-15-17 with Dol-16 dominating) was confirmed by means of HPLC/ESI-MS with application of the multiple reaction monitoring technique as well as metabolic labeling of Arabidopsis ...
Małgorzata Gutkowska-Stronkowska +6 more
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Prenylcysteine methylesterase in Arabidopsis thaliana
Gene, 2006Prenylated proteins undergo a series of post-translational modifications, including prenylation, proteolysis, and methylation. Collectively, these modifications generate a prenylcysteine methylester at the carboxyl terminus and modulate protein targeting and function.
Angela K, Deem +2 more
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Cytogenetic tools for Arabidopsis thaliana
Chromosome Research, 2003Although the first description of chromosomes of Arabidopsis dates as far back as 1907, little attention was paid to its cytogenetics for a long time. The spectacular interest in chromosome research for this species that now is the model plant species by excellence came with the introduction of molecular cytogenetical research including FISH technology,
Koornneef, M. +2 more
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QTLepi Mapping in Arabidopsis thaliana
2017While DNA sequence variation is known to be a major driver of phenotypic divergence, epigenetic variation has long been disregarded. One reason for that was the lack of suitable tools. The creation of epigenetically divergent but otherwise largely isogenic Arabidopsis populations has now alleviated some of these constraints.
Lauss, Kathrin, Keurentjes, Joost J.B.
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Accumulation of coumarins in Arabidopsis thaliana
Phytochemistry, 2006The biosynthesis of coumarins in plants is not well understood, although these metabolic pathways are often found in the plant kingdom. We report here the occurrence of coumarins in Arabidopsis thaliana ecotype Columbia. Considerably high levels of scopoletin and its beta-d-glucopyranoside, scopolin, were found in the wild-type roots.
Kosuke, Kai +4 more
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