Results 181 to 190 of about 16,348 (221)

Kinetic investigation of human 5-lipoxygenase with arachidonic acid

Bioorganic & Medicinal Chemistry Letters, 2016
Human 5-lipoxygenase (5-LOX) is responsible for the formation of leukotriene (LT)A4, a pivotal intermediate in the biosynthesis of the leukotrienes, a family of proinflammatory lipid mediators. 5-LOX has thus gained attention as a potential drug target. However, details of the kinetic mechanism of 5-LOX are still obscure.
Monica Mittal, Ramakrishnan B. Kumar, Navisraj Balagunaseelan, Mats Hamberg, Caroline Jegerschöld, Olof Rådmark, Jesper Z. Haeggström, Agnes Rinaldo-Matthis   +7 more
openaire   +3 more sources

A prenylflavone, artonin E, as arachidonate 5-lipoxygenase inhibitor

Biochemical Pharmacology, 1991
Several naturally occurring prenylflavones were tested for their inhibitory actions on arachidonate 5-lipoxygenase purified from porcine leukocytes. Of the compounds tested, artonin E (5'-hydroxymorusin) exhibited the most potent inhibition on arachidonate 5-lipoxygenase (IC50 = 0.36 microM).
G R, Reddy, N, Ueda, T, Hada, A C, Sackeyfio, S, Yamamoto, Y, Hano, M, Aida, T, Nomura   +7 more
openaire   +2 more sources

Nuclear Import of Arachidonate 5-Lipoxygenase

2001
Leukotrienes are lipid messenger molecules that are secreted by leukocytes to orchestrate a rapid and prolonged immune response. The enzyme 5-lipoxygenase catalyzes the rate-limiting first two steps in the synthesis of leukotrienes from arachidonic acid.
T G, Brock, A M, Healy
openaire   +2 more sources

A selective inhibitor of arachidonate 5-lipoxygenase scavenging peroxide activator

Biochemical Pharmacology, 1997
A novel compound termed YT-18 (2,3-dihydro-2,4,6,7-tetramethyl-2-[(4-phenyl-1-piperazinyl) methyl]-5-benzofuranamine) selectively inhibited 5-lipoxygenases of porcine leukocytes (IC50 value, 7.5 microM), human leukocytes (1.5 microM), and rat basophilic leukemia cells (14 microM), which are responsible for bioactive leukotriene synthesis.
H, Suzuki, D, Miyauchi, S, Yamamoto
openaire   +2 more sources

Arachidonic acid inhibits 5-lipoxygenase in human T cells

Biochemical and Biophysical Research Communications, 1986
The data on whether T cells produce leukotrienes or other 5-lipoxygenase metabolites of arachidonic acid is conflicting. We report that exogenous arachidonic acid added to phytohemagglutin-stimulated human T cells profoundly inhibits leukotriene B4 production, with 90% inhibition caused by 10(-6) M arachidonic acid. The 12- and 15-lipoxygenase pathways
D, Atluru, E A, Lianos, J S, Goodwin
openaire   +2 more sources

Lipoxin synthesis by arachidonate 5-lipoxygenase purified from porcine leukocytes

Biochemical and Biophysical Research Communications, 1987
Arachidonate 5-lipoxygenase purified from porcine leukocytes produced several more polar compounds from 5,15-dihydroperoxy-eicosatetraenoic acid added as such or generated from 15-hydroperoxy acid. These polar products with absorption maxima at 301-302 nm and shoulders at 289 nm and 316-317 nm were identified as 5S,6R,15S-11-cis-lipoxin A and its 6 ...
N, Ueda, S, Yamamoto, B J, Fitzsimmons, J, Rokach   +3 more
openaire   +2 more sources

Optimization of cofactors which regulate RBL-1 arachidonate 5-lipoxygenase

Biochemical and Biophysical Research Communications, 1989
The arachidonate lipoxygenase from rat basophilic leukemia cells (RBL-1) is widely utilized as a model to dissect the primary enzymatic reactions leading to leukotriene formation. The purpose of the present study was to optimize the specific activity of 5-lipoxygenase prepared from a high speed supernatant of RBL-1 cell homogenates.
F R, Cochran, M B, Finch-Arietta
openaire   +2 more sources

Colchicine inhibits arachidonate release and 5-lipoxygenase action in alveolar macrophages

American Journal of Physiology-Lung Cellular and Molecular Physiology, 1996
Although colchicine is known to inhibit leukotriene synthesis in neutrophils, its effect on other aspects of arachidonic acid (AA) metabolism as well as its mechanism of action are unknown. To address these questions, we investigated the effects of colchicine on resident rat alveolar macrophages (AM), cells that generate a variety of lipoxygenase and ...
M, Peters-Golden, R W, McNish, J A, Davis, R A, Blackwood, T G, Brock   +4 more
openaire   +2 more sources

Arachidonate 5-lipoxygenase of porcine pancreas: its localization in acinar cells

Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1991
Arachidonate 5-lipoxygenase has been found so far in various types of leukocyte. When a homogenate of porcine pancreas was incubated with arachidonic acid, 5-hydroxy-6,8,11,14-eicosatetraenoic acid was predominantly produced concomitant with small amounts of compounds derived from leukotriene A4. After differential centrifugation of the homogenate, the
K, Natsui, N, Ueda, S, Yamamoto, N, Komatsu, K, Watanabe   +4 more
openaire   +2 more sources

Effect of inhibition of 5-lipoxygenase metabolism of arachidonic acid on response to endotoxemia in sheep

Journal of Applied Physiology, 1988
We studied the effects of a 5-lipoxygenase inhibitor, L-651,192, on the pulmonary dysfunction caused by endotoxemia in chronically instrumented unanesthetized sheep. The efficacy and selectivity of L-651,392 were tested by measuring in vivo production of leukotriene B4 (LTB4) and cyclooxygenase products of arachidonic acid after endotoxemia before and
J W, Coggeshall, B W, Christman, P L, Lefferts, W E, Serafin, I A, Blair, M J, Butterfield, J R, Snapper   +6 more
openaire   +2 more sources