Results 251 to 260 of about 23,210,390 (344)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

De novo synthesis of short‐chain aldehydes and hydrocarbons secreted by the brown marmorated stink bug Halyomorpha halys

FEBS Open Bio, EarlyView.
The brown marmorated stink bug, Halyomorpha halys, mainly secretes 4‐oxo‐(E)‐2‐hexenal, (E)‐2‐decenal, dodecane and tridecane, a mixture representative of that released by many heteropterans. We demonstrated that H. halys can synthesise these secretory components de novo using dietary glucose.
Haruna Fujimori, Koji Noge
wiley   +1 more source

Pasture area increases livestock rabies risk in Rio de Janeiro, Brazil: a geospatial analysis of neurological syndrome cases (2020-2024). [PDF]

Trop Anim Health Prod
Monteiro RV, da Costa RFR, Kimura LMS, de Oliveira GR, Viana NR, de Sá Guimarães A, Silva MR, de Souza GN.   +7 more
europepmc   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Heterotropic regulation and negative homotropic cooperativity

FEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea, Francesco Angelucci, Federica Arnesano, Gianmarco Pascarella, Patrizio Di Micco, Andrea Bellelli   +5 more
wiley   +1 more source

From patient advocacy to patient‐driven research: Building active partnerships beginning at the bench to reach the bedside

FEBS Open Bio, EarlyView.
Research is strongest when conducted alongside patients, not just about them. Patient research organizations help integrate patient perspectives into research priorities, study design, and scientific meetings, leading to meaningful patient outcomes and development of relevant therapies.
Jenica H. Kakadia, Rachel Heilmann, Maximiliano Barrientos, Laura MacNeill, Ingrid Vallee, Sarah K. Schultz, Patrick O'Donoghue, Angelica A. Moresco, Victoria M. Siu, Ilka U. Heinemann   +9 more
wiley   +1 more source

Machine learning-based detection and area quantification of mechanical and corrosion damage in femoral stem tapers. [PDF]

J Mech Behav Biomed Mater
Upadhayay L, Bandyopadhyay A.
europepmc   +1 more source

TSPO-PET binding in vivo reflects increased phagocytic microglia at post mortem in people with frontotemporal dementia. [PDF]

Acta Neuropathol
Vontobel DS, Lai KO, Bacioglu M, Nolan G, Maddison DC, Adamski A, Goddard J, Shapiro NL, Crook H, Fryer T, Hong Y, Wijesinghe S, Aigbirhio F, Avezov E, Allinson KSJ, Quaegebeur A, O'Brien JT, Rowe JB, Spillantini MG, Malpetti M.   +19 more
europepmc   +1 more source

The effect of regional disparities in access to rehabilitation services on mortality among stroke patients: A Korean nationwide population-based retrospective cohort study. [PDF]

Medicine (Baltimore)
Yoo DY, Baek CY, Choi JK, Choi JK.
europepmc   +1 more source