Facile, high‐throughput protein purification enabled by high‐efficiency protease elution
Abstract While advances in artificial intelligence have made protein design widely accessible, protein production and characterization remain a bottleneck. Protease‐cleavable affinity tags are commonly used to improve yield and purity of recombinant proteins, but tag removal adds labor and complexity.
Edson N. Cárcamo Noriega +6 more
wiley +1 more source
Magnon-Magnon Interaction Induced by Dynamic Coupling in a Hybrid Magnonic Crystal. [PDF]
Sultana R +5 more
europepmc +1 more source
Navigating Classical and Nonclassical Realms of Nucleation and Crystallization
Classical nucleation and growth theories have long been foundational, yet recent nonclassical concepts have challenged and refined our understanding of solid‐state genesis. This paradigm shift introduced new, often ambiguous vocabulary. Here, we clarify key concepts, correct common misconceptions, and provide a terminological field guide across systems,
Stephan E. Wolf, Peter G. Vekilov
wiley +1 more source
EASY-C: Extraction and Analysis of Small Yeast Chromosomes-A rapid and universal platform for recovering artificial mini-chromosomes from synthetic Sc2.0 yeast and large plasmids from <i>Saccharomyces cerevisiae</i> and nonconventional yeast species. [PDF]
Swidah R, Monti M, Delneri D.
europepmc +1 more source
A novel dispersion‐phase defect‐healing strategy using Br‐doped PbS quantum dots (QDs) significantly stabilizes CsPbI3 perovskite (Pe) QDs by passivating internal vacancies. This approach reduces trap‐assisted nonradiative recombination, enabling high‐performance indoor photovoltaics with exceptional stability.
Seon Joong Kim +9 more
wiley +1 more source
The CP-PAW Code Package for First-Principles Calculations from a User's Perspective. [PDF]
Blöchl PE +7 more
europepmc +1 more source
Tunable Fabrication of 2D‐Layered Magnesium Nanosilicates With Hemostatic Properties
Tunable 2D magnesium nanosilicates form bioactive protein coronas, support cellular internalization, and promote hemostatic activity. These nanoclays enable shear‐thinning gel formation and reduce clotting time and blood loss, highlighting their potential for wound‐management applications. Tunable fabrication of inorganic two‐dimensional (2D) nanoclays
Anna L. Keller +13 more
wiley +1 more source
Dietary Supplementation of Rumen-Inert Fatty Acids and Rumen-Protected Choline Modulates the Uterine Environment of Pregnant Beef Cows. [PDF]
Bahr M +12 more
europepmc +1 more source
A 3D RNA-protein proximity ligation protocol detecting the heterogeneity of LISRR-RPL26 interactions in patient-derived melanoma tumor explants. [PDF]
Marino A, Leucci E.
europepmc +1 more source
Virtual magnetic hills to unlock the inner phases of hexagonal colloidal ice.
Baillou R, Terkel M, Tierno P.
europepmc +1 more source

