Results 261 to 270 of about 15,724 (296)

A simple chromogenic assay for arylsulfatase A

Clinica Chimica Acta, 1987
Arylsulfatase A hydrolyzes the artificial chromogenic substrate 4-nitrocatechol-sulfate at 0 degree C at a rate of 24% of that at 37 degrees C whereas arylsulfatase B is almost inactive at 0 degree C. Based on this observation, a simple assay was developed which permits the accurate determination of low residual arylsulfatase A activities in cultured ...
Ernst Conzelmann, Mary Lee-Vaupel
openaire   +3 more sources

Specific determination of arylsulfatase A activity

Experientia, 1986
Arylsulfatase activities in biological materials are too low to be detected by the methods available hitherto. A sensitive and specific assay method for arylsulfatase A (AS-A) has been developed in the present study. Ascorbate-2-sulfate is known to be a specific natural substrate of AS-A; the ascorbic acid liberated by the action of AS-A was ...
Y. Seyama, H. Inoue, S. Yamashita
openaire   +3 more sources

Microbial Synthesis of Arylsulfatase Depends on the Soluble and Adsorbed Sulfate Concentration in Soils

Social Science Research Network, 2022
T. Kunito, Hiroaki Kurita, Masaki Kumori, K. Sakaguchi, S. Nishizawa, K. Fujita, Hitoshi Moro, Kozue Sawada, Y. Miyabara, H. Toda, K. Nagaoka, Y. Ishikawa   +11 more
semanticscholar   +1 more source

Arylsulfatase A Pseudodeficiency - Incidence in Poland

European Journal of Human Genetics, 1996
Arylsulfatase A (ASA) pseudodeficiency (Pd) was defined as the in vitro measurement of low enzyme activity in a healthy person. A variable incidence of the Pd allele was found in different populations; it was 10-20 times higher than that of metachromatic leukodystrophy (MLD).
Barbara Czartoryska, Danuta Górska, Janusz Zimowski, Mariola Bisko   +3 more
openaire   +3 more sources

Pseudodeficiencies of Arylsulfatase A and Galactocerebrosidase Activities

Developmental Neuroscience, 1991
Pseudodeficiency is defined as the in vitro measurement of low activity (usually under 15% of the normal mean for controls) of an enzyme in a healthy person. They may be hard to distinguish from presymptomatic people who will present with adult-onset clinical disease.
E Louie, D A Wenger
openaire   +2 more sources

Property of arylsulfatase and β-glucuronidase extracted from digestive tracts of Cipangopaludina chinensis and their cleavage performance on conjugated natural estrogens

Environmental science and pollution research international, 2022
Kezhen Zhao, Ze-hua Liu, Jun Zhang, Shu-shu Zhong, Z. Dang   +4 more
semanticscholar   +1 more source

Arylsulfatase A Variants in Patients with Alcoholism

Alcoholism: Clinical and Experimental Research, 1984
Leukocytes from 200 mentally ill patients and 100 normal controls were analyzed for eiectrophoretic variants of arylsulfatase A. Four different variant forms were found in 15 subjects. There is a relatively high occurrence of the arylsulfatase A variants in patients with alcoholism. Twenty‐one per cent (12/56) of patients with alcoholism have a variant
Atul R. Hulyalkar, Rena Nora, Paul Manowitz   +2 more
openaire   +3 more sources

Arylsulfatase in coelomocytes ofHolothuria polii

Experientia, 1988
Holothuria polii coelomocytes possess arylsulfatase enzymes. Two pH optima were found for arylsulfatase activity in cell lysate preparations, one at pH 5.0 and the other at pH 5.8. Both increased after injection of zymosan particles or formalinized sheep red blood cells (fSR-BC), indicating an active role of the enzymes during phagocytosis of ...
openaire   +3 more sources

Arylsulfatases of human tissue studies on a form of arylsulfatase B found predominantly in brain

Biochimica et Biophysica Acta (BBA) - Enzymology, 1977
The distribution of soluble arylsulfatase (aryl-sulfate sulfohydrolases, EC 3.1.6.1) in human tissues was investigated by DEAE-cellulose chromatography, All tissues examined contained arylsulfatase A and arylsulfatase B. In addition, brain singularly contained significant quantities (15-25% of total arylsulfatase) of a minor anionic arylsulfatase from ...
Audrey R. Killgrove, Hayato Kihara, Richard L. Stevens, Arvan L. Fluharty   +3 more
openaire   +3 more sources

A new analytical bench assay for the determination of arylsulfatase a activity toward galactosyl-3-sulfate ceramide: implication for metachromatic leukodystrophy diagnosis.

Analytical Chemistry, 2014
Here, we present the design and validation of a new assay for the diagnosis of metachromatic leukodystrophy. The method is highly specific, simple, reproducible, and straightforward. In our spectrophotometric method, the determination of arylsulfatase A (
F. Morena, Ilaria di Girolamo, C. Emiliani, A. Gritti, A. Biffi, S. Martino   +5 more
semanticscholar   +1 more source