Results 311 to 320 of about 21,862 (343)
Some of the next articles are maybe not open access.
Inhibition of aspartate aminotransferase by hydrazinosuccinate
Biochimica et Biophysica Acta (BBA) - General Subjects, 1984DL-Hydrazinosuccinic acid was synthesized by the reaction of DL-bromosuccinic acid with hydrazine. The compound strongly inhibited aspartate aminotransferase and gave 50% inhibition at 1.3 microM when added simultaneously with L-aspartate to an assay mixture containing enzyme. Incubation of the enzyme with the compound prior to assay resulted in a much
Ryo-Hei Yamada +3 more
openaire +3 more sources
Aspartate aminotransferase ofLactobacillus murinus
Folia Microbiologica, 1988Aspartate aminotransferase from Lactobacillus murinus is thermostable, its activity being not changed for two months at temperatures between 4 and -70 degrees C. Maximum activity was observed at 40 degrees C and pH 7.3 in phosphate buffer (30 mmol/L). delta G* Value of 26.3 kJ/mol was calculated from the Arrhenius plot.
A. Pesce De Ruiz Holgado +3 more
openaire +3 more sources
A case of aspartate aminotransferase macroenzyme
Annals of Clinical Biochemistry: International Journal of Laboratory Medicine, 2008Aspartate aminotransferase (AST) macroenzyme can result in elevated AST activity in patients with no disease. This case report describes a six-year-old boy who presented to his family doctor with a history of chronic constipation and lower back pain.
R Jain +4 more
openaire +3 more sources
Strain and catalysis in aspartate aminotransferase
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, 2003The notion of "ground-state destabilization" has been well documented in enzymology. It is the unfavourable interaction (strain) in the enzyme-substrate complex, and increases the k(cat) value without changing the k(cat)/K(m) value. During the course of the investigation on the reaction mechanism of aspartate aminotransferase (AAT), we found another ...
Yoshitaka Nakajima +7 more
openaire +3 more sources
Measurement of Aminotransferases: Part 1. Aspartate Aminotransferase
CRC Critical Reviews in Clinical Laboratory Sciences, 1984Aminotransferases are ubiquitous enzymes of mammalian cells and several are of important diagnostic use. The application of aspartate aminotransferase activity measurements in serum from individuals suffering from myocardial infarction brought about a new dimension in clinical laboratory testing in the 1950s.
Robert Rej, Leslie M. Shaw
openaire +2 more sources
T-lymphoblastic leukaemia and aspartate aminotransferase
Leukemia Research, 1982AST levels from 11 untreated children with T-ALL were found to be significantly higher than those from 74 children with non-T disease. The enzyme was not related to haemoglobin or bilirubin levels nor to the presence of hepatosplenomegaly in any of the patients.
A.J. Kay +3 more
openaire +3 more sources
Immobilization of aspartate aminotransferase on agarose
Biochimie, 1989Various methods for immobilization of aspartate aminotransferase (AspAT; from cytosolic fraction of pig heart) on agarose were tested. Aldehyde-, thiol-, and CNBr-activated agaroses were studied in detail. The capacity of the aldehyde support to firmly bind protein was less than 0.2 mg/ml, whereas the apparent remaining specific activity of the bound ...
Timo Korpela, Kalevi Kurkijärvi
openaire +3 more sources
Aspartate aminotransferase isoenzymes
Clinical Biochemistry, 1990Aspartate aminotransferase (AST, EC 2.6.1.1) exists in human tissues as two distinct isoenzymes, one located in the cytoplasm (c-AST), and the other in mitochondria (m-AST). Striated muscle, myocardium, and liver tissues are the main sources of AST. A growing body of information suggests that determination of AST isoenzymes in human serum is useful in ...
openaire +3 more sources
Aminotransferases in Grapes. Isolation and Characterization of Aspartate Aminotransferase
American Journal of Enology and Viticulture, 1991International ...
Sauvage, F.X. +3 more
openaire +2 more sources
Stability of aspartate aminotransferase and alanine aminotransferase activities.
The Journal of laboratory and clinical medicine, 1983Because there are conflicting data regarding the effect of different temperatures and durations of storage on the stability of the activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT), a new study has been conducted to re-examine this important issue.
Hyman J. Zimmerman +4 more
openaire +2 more sources