Results 181 to 190 of about 769,865 (316)

Characterization of large transgene integrations in Chinese hamster ovary cells using a bioengineered mammalian transposase

open access: yesBiotechnology Progress, EarlyView.
Abstract We present the first use of a bioengineered mammalian transposase system derived from Myotis lucifugus (bMLT) for integration of expression vectors into the CHO genome, focusing on GFP and trastuzumab production. Initially, CHO‐K1 cells are transfected with a GFP reporter and varying amounts of bMLT DNA or mRNA.
Nicolas Marx   +10 more
wiley   +1 more source

Detergent/surfactant retention during ultrafiltration in the formulation of biotherapeutics

open access: yesBiotechnology Progress, EarlyView.
Abstract Surfactants like polysorbate (Tween®) are commonly used as excipients in the production of monoclonal antibodies and other recombinant proteins. The retention behavior of these excipients in the final ultrafiltration step can be difficult to predict due to the presence of both monomers and micelles.
Liang‐Kai Chu   +4 more
wiley   +1 more source

Minimizing batch‐to‐batch variability of a live virus vaccine by process analytical technologies

open access: yesBiotechnology Progress, EarlyView.
Abstract For bioprocesses producing live virus, such as enterovirus Coxsackievirus A21, viral titer (infectivity basis) decay rates can exceed 30% within a day. Consequently, harvest timing is paramount. To optimize titer at harvest, a continuous viral product titer model was generated to elucidate kinetics.
Katherine Forrester   +4 more
wiley   +1 more source

Community and Consumer Dynamics in NFTs: Understanding Digital Asset Value Through Social Engagement

open access: yesJournal of Consumer Behaviour, EarlyView.
ABSTRACT While non‐fungible tokens (NFTs) have emerged as a significant blockchain application, research has largely focused on market dynamics rather than consumer behavior. Through in‐depth interviews with 21 NFT consumers and a netnographic analysis of Discord interactions (109,517 words), this study develops a comprehensive framework explaining the
Kristina Brahmstaedt
wiley   +1 more source

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