Results 91 to 100 of about 299,829 (366)

Transcriptional insights on the incomplete cytoplasmic maturation and developmental potential of oocytes cultured without granulosa cells in mice

BMC Genomics
Background Oocyte maturation is crucial for female fertility and embryonic development, encompassing nuclear and cytoplasmic maturation. Supportive cells of follicles, such as granulosa cells, are essential for oocyte growth and maturation.
Yibo Wang, Yichuan Zhang, Tianjie Li, Yun Ren, Ping Zhou, Lin Fu, Chenxi Xiao, Ziying Huang, Hanji Huang, Wenfeng Xie, Yuxin Luo, Yanan Qi, Hongcui Zhao, Yang Yu, Yong Fan, Heng Pan   +15 more
doaj   +1 more source

Effect of preconceptional orlistat treatment on in-vitro fertilization outcome in overweight/obese women: study protocol for a randomized controlled trial

Trials, 2018
Background Obese women have fewer oocytes retrieved, an increased cancelation rate, a higher miscarriage rate, and a lower live birth rate after assisted reproductive technology (ART) treatment compared with women with normal weight.
Jing Li, Ze Wang, Daimin Wei, Hong Liu, Jiangtao Zhang, Jianfeng Wang, Yuhua Shi, Zi-Jiang Chen   +7 more
doaj   +1 more source

Potential of Mitochondrial Genome Editing for Human Fertility Health

Frontiers in Genetics, 2021
Mitochondrial DNA (mtDNA) encodes vital proteins and RNAs for the normal functioning of the mitochondria. Mutations in mtDNA leading to mitochondrial dysfunction are relevant to a large spectrum of diseases, including fertility disorders.
Lin Fu, Yu-Xin Luo, Ying Liu, Hui Liu, Hong-zhen Li, Yang Yu, Yang Yu   +6 more
doaj   +1 more source

Redox‐dependent binding and conformational equilibria govern the fluorescence decay of NAD(P)H in living cells

FEBS Letters, EarlyView.
In this work, we reveal how different enzyme binding configurations influence the fluorescence decay of NAD(P)H in live cells using time‐resolved anisotropy imaging and fluorescence lifetime imaging microscopy (FLIM). Mathematical modelling shows that the redox states of the NAD and NADP pools govern these configurations, shaping their fluorescence ...
Thomas S. Blacker, Nimit Mistry, Nicoletta Plotegher, Elizabeth R. Westbrook, Michael D. E. Sewell, John Carroll, Gyorgy Szabadkai, Angus J. Bain, Michael R. Duchen   +8 more
wiley   +1 more source

Expanded carrier screening in Chinese patients seeking the help of assisted reproductive technology

Molecular Genetics & Genomic Medicine, 2020
Expanded carrier screening (ECS) has emerged as an effective approach to identify at‐risk couples (ARCs)—before they initiate attempts at reproduction—who possess a high probability of having a child affected by severe recessive diseases.
Yanping Xi, Guangquan Chen, Caixia Lei, Junping Wu, Shuo Zhang, Min Xiao, Wenbi Zhang, Yue-ping Zhang, Xiaoxi Sun   +8 more
semanticscholar   +1 more source

Efficacy and safety of mesenchymal stem cell therapy for ovarian ageing in a mouse model

Stem Cell Research & Therapy
Background Ovarian ageing is one of the major issues that impacts female fertility. Mesenchymal stem cell (MSC)-based therapy has made impressive progress in recent years.
Wendi Pei, Lin Fu, Wenhuan Guo, Yibo Wang, Yong Fan, Rui Yang, Rong Li, Jie Qiao, Yang Yu   +8 more
doaj   +1 more source

Exploring lipid diversity and minimalism to define membrane requirements for synthetic cells

FEBS Letters, EarlyView.
Designing the lipid membrane of synthetic cells is a complex task, in which its various roles (among them solute transport, membrane protein support, and self‐replication) should all be integrated. In this review, we report the latest top‐down and bottom‐up advances and discuss compatibility and complexity issues of current engineering approaches ...
Sergiy Gan, Victoria Scarpelli, Marten Exterkate   +2 more
wiley   +1 more source

Sperm selection strategies and their impact on assisted reproductive technology outcomes

Andrologia, 2020
The application of assisted reproductive technologies (ART) has revolutionised the treatment of human infertility, giving hope to the patients previously considered incapable of establishing pregnancy.
S. Pinto, D. Carrageta, M. Alves, A. Rocha, A. Agarwal, A. Barros, P. Oliveira   +6 more
semanticscholar   +1 more source

Epigenetic changes and assisted reproductive technologies [PDF]

Epigenetics, 2019
Children conceived by Assisted Reproductive Technologies (ART) are at moderately increased risk for a number of undesirable outcomes, including low birth weight. Whether the additional risk is associated with specific procedures used in ART or biological factors that are intrinsic to infertility has been the subject of much debate, as has the mechanism
Sneha Mani, Jayashri Ghosh, Christos Coutifaris, Carmen Sapienza, Monica Mainigi   +4 more
openaire   +3 more sources

C‐mannosylation promotes ADAMTS1 activation and secretion in human testicular germ cell tumor NEC8 cells

FEBS Letters, EarlyView.
C‐mannosylation is a unique form of protein glycosylation. In this study, we demonstrated that ADAMTS1 is C‐mannosylated at Trp562 and Trp565 in human testicular germ cell tumor NEC8 cells. We found that C‐mannosylation of ADAMTS1 is essential for its secretion, processing, enzymatic activity, and ability to promote vasculogenic mimicry. These findings
Takato Kobayashi, Takehiro Suzuki, Ryota Kawahara, Natsumi Harai, Naoshi Dohmae, Siro Simizu   +5 more
wiley   +1 more source