Continuous use of gonadotropin-releasing hormone (GnRH)-agonist maintains high luteinizing hormone concentrations at the time of oocyte retrieval in women undergoing GnRH-agonist long protocol assisted reproductive technology treatment and stimulation with recombinant follicle-stimulating hormone [PDF]
, 2009 Shirei Ohgi +5 more
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Homologous expression and purification of human HAX‐1 for structural studies
FEBS Open Bio, EarlyView.This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley +1 more source
Psychological health of women who have conceived using assisted reproductive technology in Taiwan: findings from a longitudinal study [PDF]
, 2019 Mei‐Zen Huang +5 more
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FEBS Open Bio, EarlyView.Skin biopsies taken from a patient with an ultra‐rare disorder as well as controls were cultured for up to 473 days. The chunks of skin were serially transferred to a new culture plate when confluent with fibroblasts. Different generations of fibroblasts were analyzed for cell and molecular properties, proliferation, and competence for reprogramming to
Sudiksha Rathan‐Kumar +3 more
wiley +1 more source
FEBS Open Bio, EarlyView.This study addressed how a senior research thesis is perceived by undergraduate students. It assessed students' perception of research skills, epistemological beliefs, and career goals in Biochemistry (science) and BDC (science‐business) students. Completing a thesis improved confidence in research skills, resilience, scientific identity, closed gender‐
Celeste Suart +4 more
wiley +1 more source
The Implications of Double Human Chorionic Gonadotropin (hCG) Trigger on a Pseudo-Empty Follicle Syndrome Patient Seeking Infertility Treatment Through Assisted Reproductive Technology (ART) [PDF]
Ritesh Jadhav +4 more
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Ovarian stimulation negatively affects endometrial receptivity: a comparison of 22,504 fresh autologous vs donor oocyte IVF cycles from the society for assisted reproductive technology (SART) registry [PDF]
, 2013 Jason S. Yeh +5 more
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Risk assessment of assisted reproductive technology and parental age at childbirth for the development of uniparental disomy-mediated imprinting disorders caused by aneuploid gametes [PDF]
, 2023 Kaori Hara‐Isono +10 more
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SmallTalk: a novel small‐sized fusion tag for peptide expression and purification
FEBS Open Bio, EarlyView.The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq +3 more
wiley +1 more source