Results 231 to 240 of about 9,927,693 (264)

Asymmetric Flow Field-Flow Fractionation Investigation of Magnetopolyplexes

Macromolecular Chemistry and Physics, 2015
Asymmetric flow field-flow fractionation (AF4) is an analytical separation technique capable of providing information on different parameters of complex systems in a single measurement. Although it shows promise to be an important tool in the field of gene therapy, AF4 has only occasionally been used to study gene delivery vectors such as polyplexes ...
Haladjova E., Rangelov S., Geisler M., Boye S., Lederer A., Mountrichas G., Pispas, Stergios   +6 more
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Present situation and development trends of asymmetrical flow field-flow fractionation

Chinese Journal of Chromatography, 2017
Field-flow fractionation (FFF) is a kind of mature separation technologies in the field of bioanalysis, feasible of separating analytes with the differences of certain physical and chemical properties by the combination effects of two orthogonal force fields (flow field and external force field).
Qihui, Liang, Di, Wu, Bailing, Qiu, Nanyin, Han   +3 more
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Miniaturized asymmetrical flow field‐flow fractionation: Application to biological vesicles

Journal of Separation Science, 2007
AbstractAsymmetrical flow field‐flow fractionation (AFlFFF) has been carried out in a miniaturized channel by reducing the channel dimensions. Performance of the miniaturized AFlFFF (mAFlFFF) channel was evaluated with standard proteins and polystyrene latex spheres from nanometer to micrometer size.
Oh, Sunok., Kang, D., Ahn, S. -m., Simpson, Richard J., Lee, B. -h., Moon, MyeongHee.   +5 more
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Circular Asymmetrical Flow Field-Flow Fractionation for the Semipreparative Separation of Particles

Analytical Chemistry, 2003
A new technique for the separation and characterization of particles and polymers based on asymmetrical flow field-flow fractionation was developed. The new circular asymmetrical flow field-flow fractionation instrument (CAFFFE) resembles a quasi-parallel arrangement of 12 individual flow channels.
Michael, Maskos, Wolfgang, Schupp
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Optimization and evaluation of asymmetric flow field-flow fractionation of silver nanoparticles

Journal of Chromatography A, 2013
Asymmetric flow field-flow fractionation (AF(4)) in combination with on-line optical detection and mass spectrometry is one of the most promising methods for separation and quantification of nanoparticles (NPs) in complex matrices including food. However, to obtain meaningful results regarding especially the NP size distribution a number of parameters ...
Katrin Loeschner, Jana Navratilova, Samuel Legros, Stephan Wagner, Ringo Grombe, J. Snell, Frank von der Kammer, Erik Larsen   +7 more
openaire   +5 more sources

Size Characterization and Quantification of Exosomes by Asymmetrical-Flow Field-Flow Fractionation

Analytical Chemistry, 2015
In the past few years extracellular vesicles called exosomes have gained huge interest of scientific community since they show a great potential for human diagnostic and therapeutic applications. However, an ongoing challenge is accurate size characterization and quantification of exosomes because of the lack of reliable characterization techniques. In
Simona, Sitar, Anja, Kejžar, David, Pahovnik, Ksenija, Kogej, Magda, Tušek-Žnidarič, Metka, Lenassi, Ema, Žagar   +6 more
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Aptamer–protein binding detected by asymmetric flow field flow fractionation

Journal of Chromatography A, 2013
Asymmetric flow field flow fractionation (AF4) should be suitable for the study of aptamer-target binding, because its gentle separation would impose little disturbance to the complex structure, and it can use carrier solutions with high salt concentrations to provide the most optimal interaction environment to the complex.
Samantha, Schachermeyer, Jonathan, Ashby, Wenwan, Zhong   +2 more
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The utility of asymmetric flow field-flow fractionation for preclinical characterization of nanomedicines

Analytical and Bioanalytical Chemistry, 2019
Dynamic light scattering (DLS), transmission electron microscopy (TEM), and reversed phase-high performance liquid chromatography (RP-HPLC) are staples of nanoparticle characterization for size distribution, shape/morphology, and composition, respectively. These techniques are simple and provide important details on sample characteristics. However, DLS
Yingwen, Hu, Rachael M, Crist, Jeffrey D, Clogston   +2 more
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Flow optimisations with increased channel thickness in asymmetrical flow field-flow fractionation

Journal of Chromatography A, 2018
Retention in flow field-flow fractionation (flow FFF) is generally governed by the combination of crossflow and migration flowrates. Especially for an asymmetrical flow FFF (AF4) channel in which the channel-inlet flow is divided into crossflow and outflow, the separation of low-molecular-weight proteins or macromolecules requires a relatively high ...
Joon Seon Yang, Myeong Hee Moon
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Retention ratio and nonequilibrium bandspreading in asymmetrical flow field-flow fractionation

Analytical and Bioanalytical Chemistry, 2015
In asymmetrical flow field-flow fractionation (As-FlFFF), only the membrane-covered accumulation wall is permeable to fluid; the opposite channel wall is impermeable. Fluid enters the channel at the inlet and exits partly through the membrane-covered accumulation wall and partly through the channel outlet.
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