Application of Asymmetrical Flow Field-Flow Fractionation for Characterizing the Size and Drug Release Kinetics of Theranostic Lipid Nanovesicles. [PDF]
Int J Mol Sci, 2021 Liposome size and in vitro release of the active substance belong to critical quality attributes of liposomal carriers. Here, we apply asymmetric flow field-flow fractionation (AF4) to characterize theranostic liposomes prepared by thin lipid film ...
Skupin-Mrugalska P, Elvang PA, Brandl M.
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Physicochemical characterization and study of molar mass of industrial gelatins by AsFlFFF-UV/MALS and chemometric approach. [PDF]
PLoS ONE, 2018 Industrial gelatins have different physicochemical properties that mainly depend of the raw materials origin and the extraction conditions. These properties are closely related to the molar mass distribution of these gelatins.
Simon Duthen +6 more
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CHIMIA, 2022 Asymmetrical flow field-flow fractionation (AF4) is a powerful technique employed for the separation of macromolecules, nanoparticles, and their assemblages according to their hydrodynamic behavior.
Isabelle A. M. Worms, Vera I. Slaveykova
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A comprehensive analysis in one run - in-depth conformation studies of protein-polymer chimeras by asymmetrical flow field-flow fractionation. [PDF]
Chem Sci, 2021 Polymer-based protein engineering has enabled the synthesis of a variety of protein–polymer conjugates that are widely applicable in therapeutic, diagnostic and biotechnological industries.
Kaupbayeva B +5 more
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Asymmetrical Flow Field Flow Fractionation Coupled to Nanoparticle Tracking Analysis for Rapid Online Characterization of Nanomaterials. [PDF]
Anal Chem, 2020 Increasing applications of nanomaterials in consumer goods, industrial products, medical practices, etc., calls for the development of tools for rapid separation, quantification and sizing of nanoparticles to ensure their safe and sustainable employment.
Adkins GB, Sun E, Coreas R, Zhong W.
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Proteins and antibodies in serum, plasma, and whole blood-size characterization using asymmetrical flow field-flow fractionation (AF4). [PDF]
Anal Bioanal Chem, 2018 The analysis of aggregates of therapeutic proteins is crucial in order to ensure efficacy and patient safety. Typically, the analysis is performed in the finished formulation to ensure that aggregates are not present.
Leeman M +4 more
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Analysis and purification of ssRNA and dsRNA molecules using asymmetrical flow field flow fractionation [PDF]
Journal of Chromatography A, 2022 Robust RNA purification and analysis methods are required to support the development of RNA vaccines and therapeutics as well as RNA interference-based crop protection solutions.
Coustau, Christine +5 more
core +2 more sources
Modification of EDC method for increased labeling efficiency and characterization of low-content protein in gum acacia using asymmetrical flow field-flow fractionation coupled with multiple detectors. [PDF]
Anal Bioanal Chem, 2021 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) is widely used as a crosslinker for fluorescence labeling of protein in the fields of biochemistry and food analysis.
Zhang M, Nilsson L, Lee S, Choi J.
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Perturbations of Lipids and Oxidized Phospholipids in Lipoproteins of Patients with Postmenopausal Osteoporosis Evaluated by Asymmetrical Flow Field-Flow Fractionation and Nanoflow UHPLC–ESI–MS/MS [PDF]
Antioxidants, 2020 Osteoporosis, a degenerative bone disease characterized by reduced bone mass and high risk of fragility, is associated with the alteration of circulating lipids, especially oxidized phospholipids (Ox-PLs).
Kang Geun Lee +3 more
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Controlled Disassembly and Purification of Functional Viral Subassemblies Using Asymmetrical Flow Field-Flow Fractionation (AF4) [PDF]
Viruses, 2018 Viruses protect their genomes by enclosing them into protein capsids that sometimes contain lipid bilayers that either reside above or below the protein layer.
Katri Eskelin, Minna M. Poranen
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