Results 21 to 30 of about 8,027 (278)

Key Methodologies in Characterizing the Multi-Scale Structures of Gluten Proteins in Dough: A Comparative Review [PDF]

Biomolecules
Gluten proteins are key components in wheat flour that determine the formation of dough and the quality of flour-based products. Upon hydration and mixing, gluten proteins undergo complex structural transformations to form a gluten network, exhibiting a ...
Feifei Su, Yiyuan Zou, Zehua Zhang, Zhiling Tang, Haoran Luo, Fayin Ye, Guohua Zhao   +6 more
doaj   +2 more sources

Asymmetrical flow field-flow fractionation in purification of an enveloped bacteriophage φ 6 [PDF]

Journal of Chromatography B, 2018
Basic and applied virus research requires specimens that are purified to high homogeneity. Thus, there is much interest in the efficient production and purification of viruses and their subassemblies.
Bamford, Dennis H., Eskelin, Katri, Lampi, Mirka, Meier, Florian, Moldenhauer, Evelin, Oksanen, Hanna M., Poranen, Minna M.   +6 more
core   +4 more sources

A One‐Step Workflow for Size‐Based Separation of Extracellular Vesicles With Integrated Surface Marker Detection [PDF]

Journal of Extracellular Biology
Extracellular vesicles (EVs) are released by diverse cell types in biofluids and are increasingly studied in liquid biopsies as diagnostic and prognostic biomarkers for various diseases, including cancer.
Lien Lippens, Niké Guilbert, Sofie Van Dorpe, Sarah Deville, Robin Boiy, Quentin Roux, Nicolaas Lumen, Ilkka Miinalainen, Pekka Rappu, Katrien Vandecasteele, Hannelore Denys, Bruno De Geest, Olivier De Wever, An Hendrix   +13 more
doaj   +2 more sources

Fractionation of Nanoparticle Matter in Red Wines Using Asymmetrical Flow Field-Flow Fractionation. [PDF]

J Agric Food Chem, 2020
The particle matter of wine is mainly composed of wine colloids and macromolecules. The present work develops a methodology using asymmetrical flow field-flow fractionation coupled with multi-angle light scattering, differential refractive index detector, and ultraviolet detector (AsFlFFF-MALS-dRI-UV) for the fractionation and determination of the ...
Osorio-Macías DE, Song D, Thuvander J, Ferrer-Gallego R, Choi J, Peñarrieta JM, Nilsson L, Lee S, Bergenståhl B.   +8 more
europepmc   +4 more sources

Asymmetric flow field‐flow fractionation of liposomes: optimization of fractionation variables [PDF]

Journal of Separation Science, 2009
AbstractThe purpose of this study was to investigate the influence of ionic strength of the carrier liquid, cross flow rate, focus flow rate, and sample load on the retention behavior of liposomes in asymmetric flow field‐flow fractionation (AF4). Two differently prepared samples of large unilamellar vesicles (LUV) were used. Experiments were performed
Stefan, Hupfeld, Dominik, Ausbacher, Martin, Brandl   +2 more
openaire   +3 more sources

Revisiting the dynamics of proteins during milk powder hydration using asymmetric flow field-flow fractionation (AF4)

Current Research in Food Science, 2021
The dynamics of β-casein and casein micelles in the reconstitution of skim milk were revisited in this study. β-casein migrates into casein micelles upon an increase in temperatures due to an increase in the hydrophobic effect and lower calcium-phosphate
Anouk Lie-Piang, Mats Leeman, Alejandra Castro, Erik Börjesson, Lars Nilsson   +4 more
doaj   +1 more source

Asymmetric flow field flow fractionation methods for virus purification [PDF]

Journal of Chromatography A, 2016
Detailed biochemical and biophysical characterization of viruses requires viral preparations of high quantity and purity. The optimization of virus production and purification is an essential, but laborious and time-consuming process. Asymmetric flow field flow fractionation (AF4) is an attractive alternative method for virus purification because it is
Eskelin, Katri Johanna, Lampi, Mirka, Meier, Florian, Moldenhauer, Evelin, Bamford, Dennis Henry, Oksanen, Hanna Maarit   +5 more
openaire   +3 more sources

Screening for protein–protein interactions with asymmetrical flow field-flow fractionation [PDF]

Journal of Pharmaceutical Sciences, 2021
We describe a new method for screening protein-protein interaction of biopharmaceutical molecules at dilute concentrations to predict development issues at high concentration. The method is based on Asymmetrical Flow Field-Flow Fractionation (AF4) measurements using well known effects of protein-protein attraction on the fractionation profile due to ...
Per-Olof, Wahlund, Nikolai, Lorenzen, Christian, Rischel   +2 more
openaire   +2 more sources

Aqueous Dilution of Noble NPs Bulk Dispersions: Modeling Instability due to Dissolution by AF4 and Stablishing Considerations for Plasmonic Assays

Nanomaterials, 2020
Among different nanomaterials, gold and silver nanoparticles (AuNPs and AgNPs) have become useful tools for a wide variety of applications in general, and particularly for plasmonic assays.
Lorenzo Sanjuan-Navarro, Aaron Boughbina-Portolés, Yolanda Moliner-Martínez, Pilar Campíns-Falcó   +3 more
doaj   +1 more source

The Power of Field-Flow Fractionation in Characterization of Nanoparticles in Drug Delivery

Molecules, 2023
Asymmetric-flow field-flow fractionation (AF4) is a gentle, flexible, and powerful separation technique that is widely utilized for fractionating nanometer-sized analytes, which extend to many emerging nanocarriers for drug delivery, including lipid ...
Juan Bian, Nemal Gobalasingham, Anatolii Purchel, Jessica Lin   +3 more
doaj   +1 more source