A One-Step Workflow for Size-Based Separation of Extracellular Vesicles With Integrated Surface Marker Detection. [PDF]
Lippens L +13 more
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Introduction of Temperature-Gradient Elution in Three-Dimensional Correlation Thermal Field-Flow Fractionation with Quintuple Detection for Monitoring Compositional Dynamics of Ultrahigh-Molar-Mass Poly(styrene-<i>co</i>-maleic anhydride). [PDF]
Muza UL +4 more
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Characterisation of polymers and particles by asymmetrical flow field-flow fractionation
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The fractionation of natural nanoparticles by Asymmetrical Flow Field Flow Fractionation (As-Fl-FFF) was optimised by considering the following operating conditions: ionic strength, surfactant concentration and crossflow rate. The method performances such as fractionation recovery and fractionation efficiency were evaluated on a stable solution of ...
Gaétane Lespes +2 more
exaly +3 more sources
Field and flow programming in frit-inlet asymmetrical flow field-flow fractionation
Journal of Chromatography A, 2002The separation of wide molecular mass (Mr) ranges of macromolecules using frit inlet asymmetrical flow field-flow fractionation (FI-AFlFFF) has been improved by implementing a combination of field and flow programming. In this first implementation, field strength (governed by the cross flow-rate through the membrane-covered accumulation wall) is ...
Myeong Moon +2 more
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Asymmetric Flow Field-Flow Fractionation in the Field of Nanomedicine
Analytical Chemistry, 2014Asymmetric flow field-flow fractionation (AF4) is a widely used and versatile technique in the family of field-flow fractionations, indicated by a rapidly increasing number of publications. It represents a gentle separation and characterization method, where nonspecific interactions are reduced to a minimum, allows a broad separation range from several
Michael, Wagner +4 more
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Analysis of Peptides using Asymmetrical Flow Field-flow Fractionation (AF4)
Journal of Pharmaceutical Sciences, 2021While asymmetrical flow field-flow fractionation (AF4) has been widely used for separation of high molecular weight species and even particles, its ability to resolve lower molecular weight species has rarely been explored. Over the course of many projects, we have discovered that AF4 can be an effective analytical method for separating peptides from ...
Ryan R, Manning +6 more
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Analysis of plant ribosomes with asymmetric flow field-flow fractionation
Analytical and Bioanalytical Chemistry, 2013Ribosome profiling is a technique used to separate ribosomal subunits, 80S ribosomes (monosomes), and polyribosomes (polysomes) from other RNA-protein complexes. It is traditionally performed in sucrose gradients. In this study, we used asymmetric flow field-flow fractionation (AsFlFFF) to characterize ribosome profiles of Nicotiana benthamiana plants.
Eskelin Katri +2 more
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Fractionation of Humic Substances by Asymmetrical Flow Field-Flow Fractionation
Chromatographia, 2005Asymmetrical flow field-flow fractionation was used to investigate the effects of pH and ionic strength of the buffer, and the binding of polyaromatic hydrocarbons (PAHs) on the particle sizes of humic substances (HSs). Particle sizes were greater when HSs were present in acidic medium with phosphate buffer than when they were present in alkaline ...
G. Yohannes +3 more
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Miniaturization of Frit Inlet Asymmetrical Flow Field-Flow Fractionation
Analytical Chemistry, 2004A miniaturized frit inlet asymmetrical flow field-flow fractionation (mFI-AFlFFF) channel has been constructed and tested for the separation of proteins. By scaling down the geometrical channel dimension of a conventional FI-AFlFFF system, flow rate ranges that can be manipulated were decreased to 20-30 microL/min, which reduces the injection amount of
Dukjin, Kang, Myeong Hee, Moon
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