Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Impact of MRI modality selection on glioma sub-region segmentation using 3D U-Net and Attention U-Net: A comparative evaluation across ten MRI sequences. [PDF]
Khorasani A.
europepmc +1 more source
WRANet: wavelet integrated residual attention U-Net network for medical image segmentation. [PDF]
Zhao Y +4 more
europepmc +1 more source
Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima +7 more
wiley +1 more source
Angio-fusion net: dual-stream enhanced VGG16 attention U-Net for vessel morphology preservation in XCA segmentation. [PDF]
Sunilkumar G, Kumaresan P.
europepmc +1 more source
Attention U-net for automated pulmonary fissure integrity analysis in lung computed tomography images. [PDF]
Althof ZW +5 more
europepmc +1 more source
Heterotropic regulation and negative homotropic cooperativity
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea +5 more
wiley +1 more source
Hybrid Multi-Dimensional Attention U-Net for Hyperspectral Snapshot Compressive Imaging Reconstruction. [PDF]
Zheng S, Zhu M, Chen M.
europepmc +1 more source
Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer +7 more
wiley +1 more source
Gradient transformer Self-Attention U-Net for enhanced crack detection in concrete bridges. [PDF]
Kim B +7 more
europepmc +1 more source

