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Digital Autofocus Methods for Automated Microscopy
2006Automatic focusing of microscope images is an essential part of modern high-throughput microscopy. This chapter describes implementation of a robust autofocus system appropriate for using either air or oil immersion objectives in robotic imaging. Both hardware and software algorithms are described, and caveats of using viscous immersion media with ...
Feimo, Shen, Louis, Hodgson, Klaus, Hahn
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Autofocus Camera System For FA
SPIE Proceedings, 1988A high performance solid state TV camera with auto focusing for the purpose of assembly robot containing a robot vision, automatic visual inspection and other FA (factory automa-tion) use has been developed. The focus detection method is contrast detection method by through the lens. The contrast signal is created from a video signal.
Hiroharu Yamamoto +2 more
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Autofocus for multispectral camera using focus symmetry
Applied Optics, 2012A multispectral camera acquires spectral color images with high fidelity by splitting the light spectrum into more than three bands. Because of the shift of focal length with wavelength, the focus of each channel should be mechanically adjusted in order to obtain sharp images. Because progressive adjustment is quite time consuming, the clear focus must
Shen, HL +5 more
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A fast autofocus unit for fluorescence microscopy
Physics in Medicine and Biology, 1997In this paper a fast autofocus unit is introduced for fluorescence microscopy based on image content information. The module, an electronic board with several differentiators and integrators, is designed for high-speed autofocusing and is directly coupled to the output of the video camera.
W, Böcker +3 more
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Nanometer-level autofocus air gauge
Precision Engineering, 1998Abstract A noninvasive, pneumatic, low-flow, differential, hot-wire, top-surface sensing device is described and demonstrated for use in the Etec ALTA® mask and reticle writer. This apparatus, a nanometer-level autofocus air gauge, is capable of sensing the distance from the final objective lens to the top surface of the photoresist with greater than
Tim Thomas +3 more
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Autofocus system for microscope
Optical Engineering, 2002A technique is developed for microscope autofocusing, which is called the eccentric light beam approach with high resolution, wide focusing range, and compact construction. The principle is described. The theoretical formula of the eccentric light beam approach deduced can be applied not only to an object lens whose objective plane is just at the focal
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Autofocus system for space cameras
Optical Engineering, 2005A novel autofocusing technique using a self-imaging approach is developed for a space camera having large aperture and long focus. Its optical system consists of an emitting system, receiving system, and two pentagonal prisms. The emitting system has three parts: a light source, beamsplitter, and aim slit.
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Comparison of Phase-Gradient Autofocus and Sharpest-Image Autofocus in ISAR Imaging
2023 16th International Congress on Image and Signal Processing, BioMedical Engineering and Informatics (CISP-BMEI), 2023Zhou Wu +3 more
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