Results 111 to 120 of about 1,497,791 (338)

In vitro and in silico modelling of ROS1‐positive non‐small cell lung cancer reveals fusion‐dependent tyrosine kinase inhibitor responses

Molecular Oncology, EarlyView.
Drug resistance limits treatment success in a subset of lung cancers driven by ROS1 gene alterations. Using patient‐derived cells and computer simulations, we studied three key mutations and how they affect five targeted drugs. The mutations reduced drug effectiveness in different ways by altering protein structure and behavior.
Farhan Ul Haq, Marc Terrones, Felicia Rodrigues Fortes, Anne Schepers, Alessia Denis, Christophe Deben, Ken Op de Beeck, Guy Van Camp, Geert Vandeweyer   +8 more
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Influence of the physical properties of the liquid on axial dispersion in packed columns

The effects of the viscosity and surface tension on the axial dispersion and liq. holdup was investigated in a 0.3-m-diam. packed column with countercurrent gas-liq. flow.
Cevey, Pierre F., Von Stockar, Urs
core   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Reduced-Order Modeling of Indirect Fluidized-Bed Particle Receivers with Axial Dispersion

SolarPACES Conference Proceedings
Oxide particles present a heat transfer and thermal energy storage (TES) media for next-generation concentrating solar power (CSP) plants where the high-temperature particle TES can provide dispatchable solar power [1].
Keaton J. Brewster, Jesse R. Fosheim, Federico Municchi, Winfred R. Arthur-Arhin, Gregory S. Jackson   +4 more
doaj   +1 more source

Axial dispersion of the liquid phase in vertical tubular contactors with static mixers

, 1986
Axial dispersion characteristics in the liquid phase have been determined for three selected types of static mixers using the impulse response technique in a vertical tubular contactor for single phase (water) and two phase (water-air, cocurrent flow ...
Marie Fialová, Karl-Heinz Redlich, Kurt Winkler   +2 more
core   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

YlmG1 is localized exclusively to the chloroplast envelope membrane and is involved in preprotein translocation in Arabidopsis thaliana

FEBS Open Bio, EarlyView.
Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley   +1 more source

Estimating intra-axonal axial diffusivity with diffusion MRI in the presence of fibre orientation dispersion

, 2020
Intra-axonal axial diffusivity could be interesting biomarker of disease, yet it is often assumed constant across the white matter. Furthermore, when intra-axonal diffusivity is estimated, few models account for fibre orientation dispersion which (when ...
Mars, R.B., Khrapitchev, A.A., Mollink, J., Sibson, N.R., Sallet, J., Howard, A.F.D., Miller, K.L., Jbabdi, S.   +7 more
core  

Identifying transcription factors controlling the basal expression of human MRP4 highlights a substantial role for Sp1

FEBS Open Bio, EarlyView.
The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer, Angelique Kragl, Katrin Ziems, Juliane Glaubitz, Sophie Grammbauer, Susanne Hildebrandt, Mladen V. Tzvetkov, Gabriele Jedlitschky   +7 more
wiley   +1 more source