Results 101 to 110 of about 236,478 (267)

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

open access: yesFEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen   +9 more
wiley   +1 more source

Axially symmetric Willmore minimizers with prescribed isoperimetric ratio

open access: yesPartial Differential Equations and Applications
68 pages, comments are welcome!
openaire   +2 more sources

Molecular dynamics simulations of positively selected codons in FcγRI reveal novel biochemical binding properties

open access: yesFEBS Open Bio, EarlyView.
Evolutionary analysis across 32 placental mammals identified positive selection at residues H148 and W149 in the immune receptor FcγR1. Ancestral reconstruction combined with molecular dynamics simulations reveals how these mutations may influence receptor structure and dynamics, providing insight into the evolution of antibody recognition and immune ...
David A. Young   +7 more
wiley   +1 more source

Performance of Round-Ended Concrete-Filled Steel Tubular Columns Under Combined Compression–Bending–Shear Loading

open access: yesBuildings
This study develops and validates a finite element model for round-ended concrete-filled steel tubular (CFST) columns subjected to combined compression–bending–shear loading using ABAQUS.
Yan Peng   +4 more
doaj   +1 more source

Early‐life high‐fat diet exposure increases Achilles tendon stiffness and induces transcriptomic alterations

open access: yesFEBS Open Bio, EarlyView.
Early‐life exposure to a high‐fat diet altered intact Achilles tendons in rat offspring, making them thinner, stiffer, and molecularly distinct even without injury. These findings suggest that developmental high‐fat diet exposure may impair tendon quality and increase susceptibility to mechanical overload or tendon injury later in life.
Heyong Yin   +3 more
wiley   +1 more source

Analysis of Traveling Wave and Combined Site Effect of Long-Span Upper-Bearing Concrete-Filled Steel Tubular Arch Bridge

open access: yesAdvances in Civil Engineering
In order to study the traveling wave effect and combined site effect of long-span steel tube concrete-filled arch bridge, a 400-m span bridge of the same type is taken as an example, and a large-mass time-history analysis method with multipoint input of ...
Zite Li   +4 more
doaj   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

open access: yesFEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour   +2 more
wiley   +1 more source

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