Results 131 to 140 of about 2,004 (169)

Serological responses to Babesia bovis vaccination in cattle previously infected with Babesia bigemina

Veterinary Parasitology, 2010
Serological responses of field cattle (260) on a farm in KwaZulu-Natal, South Africa were determined before and after vaccination with the commercial Babesia bovis live-blood vaccine, using the indirect fluorescent antibody test (IFAT). All the cattle tested negative for B.
M P, Combrink, P C, Troskie, F, Du Plessis, A A, Latif   +3 more
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The immune responses to antigenic variants of Babesia bigemina in the bovine

Research in Veterinary Science, 1978
Four Babesia bigemina stabilates were used to determine the immune response of cattle to acute and chronic blood- and tick-borne infections. Thirty-two intact calves were divided into 16 groups of two and each calf was inoculated with infective B bigemina erythrocytic stabilates.
K C, Thompson, R A, Todorovict, R J, Hidalgo   +2 more
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Studies on the pathogenesis of Babesia argentina and Babesia bigemina infections in splenectomised calves

Zeitschrift f�r Parasitenkunde, 1972
The sequence of pathological changes in both B. argentina and B. bigemina infections of splenectomised calves are described. Renal damage preceded hepatic damage by one to two days. BUN levels increased 2-4 times; SGOT increased 4 times. Unconjugated bilirubin levels increased from {reversed tilde equals} 0.30 mg% to a peak 4.12 mg%±1.90 mg% in one ...
openaire   +4 more sources

Effect of Mycobacterium phlei on the development of immunity to Babesia bigemina

Veterinary Parasitology, 1996
The immunomodulatory role of Mycobacterium phlei against intracellular blood protozoan Babesia bigemina was demonstrated following experimental immunisation and challenge in bovine calves. A lysate of erythrocytes infected (6 x 10(9)) with B. bigemina was used as a source of dead antigen either with Freund's complete adjuvant (FCA) or with a ...
A K, Tewari, N N, Sharma, J R, Rao, A K, Mishra, S K, Das   +4 more
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Fluorescent Antibody Tests for Babesia bigemina and B. berbera

Research in Veterinary Science, 1972
Summary The indirect fluorescent antibody method has been used to detect antibodies in cattle against Babesia bigemina and B. berbera, and as a means of identifying Babesia species in internal organs of cattle. Test antigen was prepared from washed, hacmolyzed blood in the case of B. bigemina, and from infected kidney in the case of B.
M, Goldman, E, Pipano, A S, Rosenberg
openaire   +2 more sources

Development of Tick Gut Forms of Babesia bigemina In Vitro

Journal of Eukaryotic Microbiology, 1998
Development of a laboratory cultured tick‐transmissible strain of Babesia bigemina was followed in vitro after addition of gut material from engorged female Boophilus microplus ticks and incubation at 37° C. Sequential development of stages, from intraerythrocytic strahlenkörper through multiplication to the fusion of what is assumed to be two gametes,
Golgh, Joanne M., Jorgensen, Wayne K., Kemp, David H.   +2 more
openaire   +3 more sources

Expression and characterization of the Babesia bigemina cysteine protease BbiCPL1

Acta Tropica, 2012
BbiCPL1 was the first papain-like cysteine protease from a piroplasm to be identified with proteolytic activity. Here we report the improved production of the active recombinant enzyme, and the biochemical characterization of this potential drug target. BbiCPL1 showed characteristic properties of its class, including hydrolysis of papain-family peptide
Tiago M, Martins, Virgílio E, do Rosário, Ana, Domingos   +2 more
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Improved Molecular Tools for Detection of Babesia bigemina

Annals of the New York Academy of Sciences, 2008
Molecular detection of Babesia bigemina involves a nested PCR protocol and reverse line blot hybridization (RLBH) assay based on the 18S gene. In this study, we report the development of molecular tools for improving B. bigemina detection in bovine blood—a one‐step PCR assay based on the amplification of rap‐1a paralogous and a new RLBH Babesia spp ...
Romina, Petrigh, Paula, Ruybal, Carolina, Thompson, Roberto, Neumann, Rosalia, Moretta, Silvina, Wilkowsky, Graciela, Draghi, Ignacio, Echaide, Susana Torioni, de Echaide, Marisa, Farber   +9 more
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Immunochemical characterization of in vitro culture-derived antigens of Babesia bovis and Babesia bigemina

Veterinary Parasitology, 1998
Cross-reactivity between Babesia bovis and B. bigemina becomes a problem in discrimination of the two infections in endemic areas where the two species usually occur in association. With the aim of identifying candidate proteins for use as specific diagnostic tools, culture-derived components of three geographically different stocks of B.
L M, Passos, L, Bell-Sakyi, C G, Brown
openaire   +2 more sources

Development of an In vitro Microtest to Assess Drug Susceptibility of Babesia bovis and Babesia bigemina

The Journal of Parasitology, 1991
Continuous cultivation of the bovine hemoparasites Babesia bovis and Babesia bigemina was developed as an in vitro microtest to assess parasite susceptibility to babesicidal compounds. Reproducibility of parasite multiplication rates was independent of culture size, making it possible to use a microscale of 100 microliters for each test sample ...
C R, Brockelman, P, Tan-ariya
openaire   +2 more sources