Results 231 to 240 of about 9,397 (265)
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THE ENZYMES OF PYRIMIDINE BIOSYNTHESIS IN BABESIA BOVIS AND BABESIA BIGEMINA
Australian Journal of Experimental Biology and Medical Science, 1983SummaryAll six enzymes of de novo pyrimidine biosynthesis leading to the formation of UMP have been demonstrated in whole homogenates from two bovine Babesia species, B. bovis and B. bigemina. The specific activities of the respective enzymes were of the same order of magnitude as observed for the related parasite, Plasmodium berghei.
Annette M. Gero +3 more
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Babesia bovis: The development of an expression oligonucleotide microarray
Experimental Parasitology, 2007The availability of a stage-specific Babesia bovis expression profile can facilitate the identification of candidate vaccine antigens. In addition, highly expressed genes during a particular developmental stage may suggest their relevance during that stage. In this study, we generated and validated a custom B.
Audrey O.T. Lau +2 more
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Transient transfection of purified Babesia bovis merozoites
Experimental Parasitology, 2008Transient transfection of intraerythrocytic Babesia bovis parasites has been previously reported. In this study, we describe the development and optimization of methods for transfection of purified B. bovis merozoites using either nucleofection (Amaxa) or conventional electroporation (Gene Pulser II, BioRad).
Carlos E. Suarez, Terry F. McElwain
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Human serum for in vitro cultivation of Babesia bovis
Veterinary Parasitology, 1992Various combinations of human serum (from blood of groups A and Rhesus positive) with bovine serum, i.e. 20% + 20% (Medium I), 30% + 10% (II), 40% + 0% (III) and 0% + 40% (IV) and Medium-199 (60%) were used in the propagation of Babesia bovis. Babesia bovis stabilate revived by inoculation in a bovine calf was used at a level of 6% parasitized ...
I. Kakoma, A. K. Mishra, G. Clabaugh
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PCR methods for the discrimination of Babesia bovis isolates
Veterinary Parasitology, 1997Three different polymerase chain reaction assays for the typing of isolates of Babesia bovis have been developed and compared with a hybridisation based method. Primers were designed within conserved regions flanking the variable length tandem repeats of the Bv80 and BvVA1 genes.
Lew, Ala E. +3 more
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Immunopathophysiology of Babesia bovis and Plasmodium falciparum infections
Parasitology Today, 1988Babesia bovis and Plasmodium falciparum are both vector-borne parasites primarily infecting the erythrocytes of their respective hosts. They have obvious differences, yet the diseases caused by these parasites share many common features. Both have generated a considerable body of research but, perhaps because of the classical distinction between ...
Ian A. Clark, I.G. Wright, B.V. Goodger
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Enzydmatic Characterization of Babesia bovis1
The Journal of Protozoology, 1986ABSTRACT. Agarose gel electrophoresis was used to identify metabolic enzymes in Babesia bovis and B. bigemina. Glutamate dehydrogenase, lactate dehydrogenase, glucose phosphate isomerase, and hexokinase were identified in B. bovis‐ and B. bigemina‐infected erythrocytes and B. bovis merozoite preparations.
S D Rodriguez +3 more
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Procoagulant Activity of Babesia bovis-Infected Erythrocytes
The Journal of Parasitology, 1987transmitted the trypanosome by introduction of infected mites per os to Serinus canaria. The presence of epimastigotes and metacyclic trypanosomes in 3 species of mites that are associated with moles and the growth of these flagellates to trypnomastigotes in culture at 37 C, which resemble the bloodstream form of T. talansmi ted the trypanosome by intr
Rode-Bramanis K +2 more
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Growth of Babesia Bovis in Bovine Erythrocyte Cultures
The American Journal of Tropical Medicine and Hygiene, 1978Babesia bovis was cultured in a suspension of bovine erythrocytes incubated at 37 degrees C in Medium 199 with 50% bovine serum. The cells in culture were kept in suspension by slow stirring in spinner flasks and the medium was replaced at 24-hour intervals.
B.M. Osorno +5 more
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Babesia bovis host cell recognition proteins
International Journal for Parasitology, 1991Babesia bovis enters host erythrocytes by invagination but nothing is known of the proteins involved. By means of metabolic labelling, differential centrifugation in oil and salt elution, a number of babesial proteins have been shown to bind to bovine erythrocytes. Strong binding is evidenced only by a 38/19 kDa pair.
B. Garrone +4 more
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