Results 141 to 150 of about 14,273,081 (281)
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
C.S.I. on the Deep Reef (Chemotrophic Species Investigations) [PDF]
In this lesson students discover what factors are indicative of chemotrophic nutritional strategies. Students will be able to describe at least three chemotrophic symbioses known from deep-sea habitats.
Mel Goodwin
core
FEBS Open Bio, EarlyView.This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
Steamship Portland: Fetch Me a Wave [PDF]
In this lesson students discover how ocean waves form and the effect of extreme storms on wave formation. They will investigate the "Portland Gale" of 1898, the Halloween Nor'easter of 1991 (known as "The Perfect Storm"), and discuss hybrid storms and ...
Mel Goodwin
core
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
The Big Burp: Where's the Proof? [PDF]
This lesson focuses on the possible release of methane from methane hydrates to the atmosphere and the potential impact of these releases on global warming, particularly how they might be involved with the Cambrian explosion and the Paleocene extinction ...
core
FEBS Open Bio, EarlyView.Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel +8 more
wiley +1 more source
Surface topography, including the various physical methods of measuring surfaces, and the various lubrication regimes (hydrodynamic, elastohydrodynamic, boundary, and mixed) are discussed.
Dowson, D., Hamrock, B. J.
core +1 more source
FEBS Open Bio, EarlyView.Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley +1 more source
Mountains in the Sea: No Escape [PDF]
In this activity, students will explore the fate of benthic invertebrate larvae in the vicinity of seamounts. They will review field data to evaluate a hypothesis about the influence of a water circulation cell on the retention of benthic invertebrate ...
core