Results 101 to 110 of about 7,086 (292)

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Backsliding Populist Governments in the Council: The Case of the Hungarian Fidesz

Politics and Governance
Populist governments aim to fundamentally challenge the EU, raising the question of when and how backsliding populist governments disrupt decision-making in the Council of the EU (hereafter Council).
Ramona Coman
doaj   +1 more source

Why Masses Support Democratic Backsliding

Concerns over democratic backsliding have proliferated recently, as elected politicians have sought to undermine democratic checks and balances. This study examines the underpinnings of public support for democratic backsliding, delineating five ...
Lior Sheffer, Itamar Yakir, Yotam Margalit, Noam Gidron   +3 more
core   +1 more source

Guiding AlphaFold to predict how Munc13‐1 opens Syntaxin‐1

FEBS Open Bio, EarlyView.
The syntaxin‐1 Habc‐domain (orange), linker (pink) and SNARE motif (yellow) form a closed conformation that binds to Munc18‐1 (violet) and is opened by the Munc13‐1 MUN domain (cyan) to form the SNARE complex that triggers neurotransmitter release.
Madhurima Chattopadhyay, Junjie Xu, Josep Rizo   +2 more
wiley   +1 more source

Evaluating the involvement of autolysosomes in the nuclear translocation of fluorescent proteins

FEBS Open Bio, EarlyView.
Endogenously expressed fluorescent proteins can be degraded by autophagy and transported to cell nuclei via the nuclear pore complex. But in some cell lines, for example, HeLa cells which are positive for immunoreactivity of a receptor ligand, such as UCN I, in cell nuclei, fusion of autolysosome with the nuclear envelope is involved in the nuclear ...
Keiichi Ikeda
wiley   +1 more source

Countering Disinformation Amid Democratic Backsliding: Difficult Comparisons Between the European Union and Serbia

Journalism and Media
This research examines the contemporary policy framework through which the European Union seeks to counter-disinformation, situating this agenda within the broader challenges posed by ongoing democratic backsliding.
Irina Milutinović, Aleksandra Krstić
doaj   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source

Gay Rights in the Age of Democratic Backsliding: Bolsonaro’s Brazil with Reflections on the United States

Latin American Research Review
Democratic backsliding is widely recognized as a threat to gay rights, but it is far from clear why the impact of this political malaise varies from country to country.
Omar G. Encarnación
doaj   +1 more source

Administrative backsliding

Democratic administration can be conceived as a political institution of the state in its own right. As part of executive government democratic administration is exposed to backsliding pressures at an early stage of autocratization trajectories. The chapter discusses how bureaucratic transformation to lower levels of democratic quality – defined as ...
openaire   +2 more sources