Results 71 to 80 of about 7,086 (292)
FEBS Letters, EarlyView.We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace +6 more
wiley +1 more source
Reconstructing enzyme evolution by protein engineering
FEBS Letters, EarlyView.Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler +2 more
wiley +1 more source
Ligand‐dependent transcriptional heterogeneity in cell cycle gene expression delays G1/S entry
FEBS Letters, EarlyView.EGF and HRG induce distinct G1/S progression programs in ErbB2‐amplified BT474 breast cancer cells. Despite activating the potent ErbB2–ErbB3 heterodimer, HRG does not accelerate cell‐cycle entry. Instead, EGF promotes earlier restriction‐point passage via ERK–FOS signaling, whereas HRG activates the AKT–MYC axis, driving transcriptional heterogeneity ...
Ririn Rahmala Febri +5 more
wiley +1 more source
FEBS Letters, EarlyView.How do genomes gain new functional parts? In eukaryotes, which tend to evolve under weak selection, much of the genome is junk. Palazzo and Qiu borrow the logic of Markov chains to show how non‐functional DNA becomes functional through the appearance of intermediate states, which arise due to epistasis, buffering, and biochemical messiness, allowing ...
Alexander F. Palazzo, Yi Qiu
wiley +1 more source
Democratic Stability and Backsliding
Political PhilosophyIn light of mounting concerns about democratic backsliding, Rawls’s work – which has an unusual focus on considerations of stability – is now being mined for insights about democratic fragility.
Ryan Pevnick
doaj +2 more sources
Metastatic niche shaped by host factors influences disseminated cancer cell fate
FEBS Letters, EarlyView.Metastasis is shaped not only by cancer cells but also by the environments they encounter. This review explores how factors such as aging, diet, the microbiome, lifestyle, and environmental exposures remodel organ‐specific niches in the lung, liver, bone, and brain, influencing where metastatic cells survive, remain dormant, or grow, and ultimately ...
Gwennan Delyth Ward +2 more
wiley +1 more source
A Wounded Democracy: Analysis of the Determinant Factors of Democratic Backsliding in Brazil
Cosmopolitan Civil Societies: An Interdisciplinary JournalDespite its prominence in the international political landscape and its widespread adoption throughout the world, democracy is seldom a stable regimen, and often repeatedly stressed and tested by attempts to wield autocratic power.
Davi Mendes Málaga +1 more
doaj +1 more source
Democratic Backsliding and Parliament: An Analysis of the Polish Sejm
, 2023 As we enter the Third Wave of Autocratisation, democratic backsliding is on the rise. Current research has emphasised the causes of backsliding to the detriment of its components, with the role of the legislature curiously absent from most work in this ...
Scott, Greta Charlotte Francesca
core
Inhibition of dipeptidyl peptidase 7 impairs lysophagy in LLOMe‐treated HepG2 cells
FEBS Letters, EarlyView.DPP7 inhibition delays ubiquitin‐dependent lysophagy by attenuating ubiquitin accumulation and the recruitment of SQSTM1 and LC3 to damaged lysosomes, resulting in delayed clearance of damaged lysosomes and impaired restoration of lysosomal homeostasis. Created using FigureLabs (Certificate ID: FL‐PUB‐20260922‐Z99XNX).
Na Yeon Park +8 more
wiley +1 more source
Democratic backsliding and resilience during a pandemic: the cases of Poland and Italy
Italian Political ScienceWe investigate the consequences of the Covid-19 crisis on the quality and survival of democracy in a country. We start from the idea that such crises entail a risk of democratic backsliding, as governments could exploit the state of emergency to ...
Andrea Cassani +3 more
doaj +2 more sources