Results 71 to 80 of about 383,766 (258)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Bacterial community composition and extracellular enzyme activity in temperate streambed sediment during drying and rewetting [PDF]

, 2013
Droughts are among the most important disturbance events for stream ecosystems; they not only affect stream hydrology but also the stream biota. Although desiccation of streams is common in Mediterranean regions, phases of dryness in headwaters have been
Pohlon, Elisabeth, Ochoa Fandino, Adriana, Marxsen, Jürgen   +2 more
core   +2 more sources

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

Soil bacterial and fungal communities across a pH gradient in an arable soil [PDF]

, 2010
Soils collected across a long-term liming experiment (pH 4.0-8.3), in which variation in factors other than pH have been minimized, were used to investigate the direct influence of pH on the abundance and composition of the two major soil microbial taxa,
AE McCaig, AK Shaw, C Lozupone, C Lozupone, Catherine Lozupone, Christian L Lauber, CL Lauber, CL Lauber, DL Kirchman, E Bååth, E Bååth, E Pruesse, ED Jesus, Erland Bååth, GW Nicol, J Borneman, J Gregory Caporaso, J Gregory Caporaso, J Rousk, J Rousk, J Rousk, J Rousk, JC Aciego Pietri, JC Aciego Pietri, JC Aciego Pietri, Johannes Rousk, JP Davis, KA Wheeler, KH Domsch, KL Baker, L Nevarez, L Philippot, L Rosso, LO Nilsson, LT Bennett, M Buée, M Hamady, M Hamady, MK Männistö, MN Price, N Beales, N Fierer, N Fierer, N Fierer, N Fierer, N Fierer, Noah Fierer, PA Dimitriu, PE Axelrood, Philip C Brookes, Rob Knight, RT Jones, S Fazi, SD Frey, V Kunin, W Li, WH Hartman   +56 more
core   +1 more source

A Systematic Comparison of Alpha‐Synuclein Seed Amplification Assays for Increasing Reproducibility

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Seed amplification assays (SAAs) enable ultrasensitive detection of misfolded α‐synuclein across biofluids and tissues. Yet, heterogeneity in protocols limits cross‐study comparability and clinical translation. Here, we review α‐synuclein SAA methods and their performance across various biological matrices.
Manuela Amaral‐do‐Nascimento, Daniela F. Santos, Tuane C. R. G. Vieira, Tiago F. Outeiro   +3 more
wiley   +1 more source

Bacterial and fungal community composition and community-level physiological profiles in forest soils

PLOS ONE, 2023
We characterized the potential functioning and composition of the bacterial and fungal communities in the O and A horizons of forest soils using community-level physiological profile (CLPP) based on BIOLOG analysis, and polymerase chain reaction–denaturing gradient gel electrophoresis (PCR-DGGE) analysis of 16S and 18S rDNA fragments, respectively.
Takashi Kunito, Shota Hibino, Hirotaka Sumi, Kozue Sawada, Ho-Dong Park, Kazunari Nagaoka   +5 more
openaire   +4 more sources

Block Copolymers: Emerging Building Blocks for Additive Manufacturing

Advanced Functional Materials, EarlyView.
This review addresses how block copolymer (BCP) physics and rheology have led to the widespread use of BCPs in advanced additive manufacturing techniques, with particular emphasis on the untapped potential of these nanostructured materials toward achieving multi‐scale architected materials with unique, programmable material properties.
Alice S. Fergerson, Hemish B. Thakkar, Jiaen Wu, Emily C. Davidson   +3 more
wiley   +1 more source

Significant Differences in the Gut Bacterial Communities of Hooded Crane (Grus monacha) in Different Seasons at a Stopover Site on the Flyway

Animals, 2020
Intestinal bacterial communities form an integral component of the organism. Many factors influence gut bacterial community composition and diversity, including diet, environment and seasonality.
Fengling Zhang, Xingjia Xiang, Yuanqiu Dong, Shaofei Yan, Yunwei Song, Lizhi Zhou   +5 more
doaj   +1 more source

Assessment of Bacterial Community Assembly Patterns and Processes in Pig Manure Slurry. [PDF]

PLoS ONE, 2015
The bacterial community assembly patterns and processes are poorly understood in pig manure slurry. We collected pig manure slurry samples during the winter and summer seasons from eight commercial pig farms in South Korea.
Priyanka Kumari, Hong L Choi, Sartika I A Sudiarto   +2 more
doaj   +1 more source

Patterns of Oral Microbiota Diversity in Adults and Children: A Crowdsourced Population Study. [PDF]

, 2020
Oral microbiome dysbiosis has been associated with various local and systemic human diseases such as dental caries, periodontal disease, obesity, and cardiovascular disease.
Burcham, Zachary M, Comstock, Sarah S, Garneau, Nicole L, Genetics of Taste Lab Citizen Scientists, Knight, Rob, Metcalf, Jessica L, Tucker, Robin M   +6 more
core