Results 31 to 40 of about 10,374 (168)
Nature Communications, 2023 Tomato is the highest value fruit and vegetable crop worldwide, yet produces α-tomatine, a renowned toxic and bitter-tasting anti-nutritional steroidal glycoalkaloid (SGA) involved in plant defense. A suite of modifications during tomato fruit maturation
Prashant D. Sonawane +10 more
doaj +2 more sources
Different strategies to enhance the solubility of acylsucrose transferase PaxASAT1 expression in Escherichia coli [PDF]
Biochemistry and Biophysics ReportsHeterologous expression of soluble proteins is a crucial method for studying gene function and enzyme structure. Escherichia coli is a widely used host for expressing foreign genes from plants, animals, and microorganisms.
Li Han +8 more
doaj +2 more sources
The BAHD Acyltransferase Gene Family: Evolutionary Dynamics, Biochemical Mechanisms, and Roles in Plant Stress Adaptation. [PDF]
Plant Biotechnol JBAHD acyltransferases drive metabolic diversification in plants by coupling conserved catalytic scaffolds with regulatory flexibility, enabling stress adaptation and ecological specialisation. ABSTRACT BAHD acyltransferases constitute one of the most versatile enzyme superfamilies in plants, catalysing the acylation of alcohols, amines, polyamines, and
Zafar MM +7 more
europepmc +2 more sources
New Phytologist, 2015 SummaryPhenolic compounds are secondary metabolites involved in several plant growth and development processes, including resistance to biotic and abiotic stresses. The biosynthetic pathways leading to the vast diversity of plant phenolic products often include an acylation step, with phenolic compounds being the donor or acceptor molecules.
Bontpart, Thibault +3 more
openaire +5 more sources
Combined metabolome and transcriptome to analyze the regulatory network of key enzymes in the synthesis of senkirkine in Emilia sonchifolia [PDF]
BMC Plant BiologyEmilia sonchifolia (L.) DC. serves as a well-known folk medicinal and edible plant, yet its toxic component senkirkine exhibits hepatotoxicity. Acute high-dose exposure can induce sinusoidal obstruction syndrome, while chronic low-dose intake may cause ...
Jinli Yao +8 more
doaj +2 more sources
BAHD acyltransferase from dragon fruit enables production of phyllocactin in engineered yeast. [PDF]
FEMS Yeast ResAbstract Microbial fermentation can provide a sustainable and cost-effective alternative to traditional plant extraction to produce natural food colours. Betalains are a class of yellow to red water-soluble pigments. Even though over 80 betalain variants are known, betanin is the only betalain available as a food colourant on the market.
Glitz C +4 more
europepmc +4 more sources
pHBMT1, a BAHD-family monolignol acyltransferase, mediates lignin acylation in poplar [PDF]
Plant Physiology, 2021 Abstract Poplar (Populus) lignin is naturally acylated with p-hydroxybenzoate ester moieties. However, the enzyme(s) involved in the biosynthesis of the monolignol–p-hydroxybenzoates have remained largely unknown. Here, we performed an in vitro screen of the Populus trichocarpa BAHD acyltransferase superfamily (116 genes) using a ...
Lisanne de Vries +12 more
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Identification and characterization of BAHD hydroxycinnamoyltransferases in the fern Neoblechnum brasiliense. [PDF]
Plant JSignificance Statement Nine BAHD acyltransferases have been identified and characterized in the fern Neoblechnum brasiliense transferring hydroxycinnamoyl units onto various alcohol and amine acceptor substrates. Kinetic characterization showed that shikimic and quinic acids for ester formation as well as 3‐hydroxyanthranilic acid and tryptamine for ...
Ufland M, Petersen M.
europepmc +2 more sources
Nature Communications, 2023 Plant biosynthetic enzymes rapidly evolve to catalyze specialized reactions. Here, the authors present the crystal structure and mechanism of COSY, the enzyme involved in coumarin biosynthesis of the BAHD-acyltransferase family that catalyzes an ...
Colin Y. Kim +7 more
doaj +1 more source
The Last Step in Cocaine Biosynthesis Is Catalyzed by a BAHD Acyltransferase [PDF]
Plant Physiology, 2014 The esterification of methylecgonine (2-carbomethoxy-3β-tropine) with benzoic acid is the final step in the biosynthetic pathway leading to the production of cocaine in Erythoxylum coca. Here we report the identification of a member of the BAHD family of plant acyltransferases as cocaine synthase.
Gregor Wolfgang Schmidt +10 more
openaire +4 more sources