Results 81 to 90 of about 5,621 (267)

Identification of the Subcompartment-Specific Mitochondrial Proteome by APEX2 Proximity Labeling in Saccharomyces cerevisiae

open access: yesBio-Protocol
The cellular compartments of eukaryotic cells are defined by their specific protein compositions. Different strategies are used for the identification of the subcellular proteomes, such as fractionation by differential centrifugation of cellular extracts.
Lorenz Spänle, Johannes Herrmann
doaj   +1 more source

High‐Throughput Generation of Tumor Spheroids via Droplet Microfluidics for siRNA‐Loaded Nanomedicine Assessment

open access: yesAdvanced Healthcare Materials, EarlyView.
A droplet microfluidic platform is employed to enable high‐throughput, uniform tumor spheroid generation for evaluating siRNA‐loaded nanomedicines at the protein level. As a proof of concept, breast (MCF‐7) and brain (U87 MG) cancer cell lines are investigated using this platform, revealing penetration profiles and therapeutic responses between the two
Ling Liu   +4 more
wiley   +1 more source

Boron‐Doped Nano‐Crystalline Coated Carbon Fibers for Phasic Dopamine Sensing

open access: yesAdvanced Healthcare Materials, EarlyView.
This uniform coating presents a mix of graphitic and diamond‐like carbon to create a mechanically and electrically robust alternative for the electrochemical detection of dopamine. The electrodes in this work possess the equivalent sensitivity to the state‐of‐the‐art FSCV dopamine detecting electrodes, whilst demonstrating an ability to withstand ...
Simon J. Higham   +19 more
wiley   +1 more source

Advanced 3D Platforms for Modeling CNS Neuroinflammation: Cell Integration, Techniques, and Challenges

open access: yesAdvanced Healthcare Materials, EarlyView.
This review summarizes the roles of key central nervous system cell types, the extracellular matrix, and the blood‐brain barrier in neuroinflammation, and their integration into diverse 3D culture systems. It examines major incorporation strategies, including direct co‐culture, hydrogel encapsulation, transwell migration assays, and bioprinting ...
Emmanuelle D. Aiyegbusi   +2 more
wiley   +1 more source

A Synovium‐on‐Chip Platform to Study Multicellular Interactions in Arthritis

open access: yesAdvanced Healthcare Materials, EarlyView.
The Synovium‐on‐Chip comprises a thin microporous PDMS membrane to support co‐culture of fibroblast‐like synoviocytes (FLS), THP‐1‐derived macrophages, and endothelial cells, enabling real‐time analysis of synovial‐vascular interactions. FLS migration through the pores drives endothelial remodeling, while TNF‐α stimulation induces robust inflammatory ...
Laurens R. Spoelstra   +8 more
wiley   +1 more source

Technological aspects of managing the structural and mechanical properties of wheat bread crumb

open access: yesПищевые системы
This work is dedicated to the study of the structural-mechanical properties of wheat bread crumb 12 hours after baking and then every 24 hours during 108 hours of storage. The basis of this investigation was the study of the starch retrogradation process,
V. Ya. Chernykh   +4 more
doaj   +1 more source

Comparison of Alcohol Free GSH Production by Ultrasonic and Homogenizing Method [PDF]

open access: yesIranian Journal of Chemistry & Chemical Engineering, 2008
Homogenizer and ultrasonicator that we call as mechanical methods were used in the lab scale production of alcohol free reduced glutathione (alcohol free GSH) from baker’s yeast.
Mohamad Ramlan Mohamed Salleh   +4 more
doaj  

Oxygen and ROS Delivery for Infected Wound Healing and Future Prospects

open access: yesAdvanced Healthcare Materials, EarlyView.
Bacterial infection is a major driver of delayed wound healing and postsurgical readmissions; with rising antibiotic resistance, solid peroxide–releasing biomaterials offer sustained delivery of ROS/O2 for antimicrobial control and microenvironmental modulation.
Ayden Watt   +7 more
wiley   +1 more source

Temporally Delayed Deployment of Photo‐Responsive Liquid Crystal Polymer Networks Toward Neural Interfaces

open access: yesAdvanced Healthcare Materials, EarlyView.
Deployable medical devices typically need external stimuli to trigger deployment. However, external stimuli are difficult to supply within tissues. Here, we describe a strategy to deploy small‐scale structures into soft tissues after insertion without the need for any stimulus. We demonstrate deployment within a tissue phantom.
Yeh‐Chia Tseng   +13 more
wiley   +1 more source

Sourdough authentication: quantitative PCR to detect the lactic acid bacterial microbiota in breads

open access: yesScientific Reports, 2017
No national legislation anywhere in the world regulates and protects traditional/typical sourdough breads. Sourdough fermentation is firmly associated with a century-old tradition, and with sensory and nutritional quality of breads.
Erica Pontonio   +6 more
doaj   +1 more source

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