Results 31 to 40 of about 380,630 (261)
FEBS Letters, EarlyView.We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace +6 more
wiley +1 more source
BARRIER-FREE DESIGN AS ELEMENT OF ECOLOGICAL ARCHITECTURAL ENVIRONMENT [PDF]
Current problems of architecture and urban planning, 2020 У статті розглядається взаємозв'язок концепції інклюзивного дизайну, зокрема формування міського середовища шляхом побудови безбар'єрних маршрутів та ареалів, з принципами сталого розвитку та формуванням сталої громади в архітектурному ...
openaire +2 more sources
Reconstructing enzyme evolution by protein engineering
FEBS Letters, EarlyView.Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler +2 more
wiley +1 more source
Investigating transcription factor dynamics in health and disease using FRAP
FEBS Letters, EarlyView.FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj +3 more
wiley +1 more source
Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential
FEBS Letters, EarlyView.Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta +3 more
wiley +1 more source
Encouraging a 'Barrier-free Built Environment' in a Malaysian University [PDF]
Journal of Design and the Built Environment, 2005 A good pedestrian network around the campus should be accessible and friendly for all users including disabled persons. The environment should offer some activity nodes to ensure that the learning and working in campus is more pleasant.
Hazreena Hussein
doaj
Dynamic energy landscapes of riboswitches help interpret conformational rearrangements and function. [PDF]
PLoS Computational Biology, 2012 Riboswitches are RNAs that modulate gene expression by ligand-induced conformational changes. However, the way in which sequence dictates alternative folding pathways of gene regulation remains unclear.
Giulio Quarta, Ken Sin, Tamar Schlick
doaj +1 more source
Structure‐forward targeting of claudins with synthetic binders
FEBS Letters, EarlyView.Claudins form the paracellular barriers between epithelial and endothelial tissues at tight junctions and are targets for molecular binders with the goal of modulating barrier permeability. Claudin‐binding molecules are relevant in drug delivery or in altering claudin interactions with disease‐causing proteins.
Alex J. Vecchio
wiley +1 more source
Beyond PFAS: Fluorine-free materials for sustainable high-performance surface and barrier materials
Materials Today SustainabilityPer- and polyfluoroalkyl substances (PFAS) have long enabled high-performance surface and barrier materials due to their exceptional chemical stability, durability, and ultra-low surface energy arising from the carbon–fluorine bond.
Ewomazino Ojogbo +2 more
doaj +1 more source
Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation
FEBS Letters, EarlyView.Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang +2 more
wiley +1 more source