Results 241 to 250 of about 32,167,916 (377)

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

BLOOD ALCOHOL LEVELS IN RATS: NON‐UNIFORM YIELDS FROM INTRAPERITONEAL DOSES BASED ON BODY WEIGHT [PDF]

, 1982
Floyd E. Bloom, PUSHKARAJ LAD, Quentin J. Pittman, Joseph Rogers   +3 more
openalex   +1 more source

Thrombolytic proteins profiling: High‐throughput activity, selectivity, and resistance assays

FEBS Open Bio, EarlyView.
We present optimized biochemical protocols for evaluating thrombolytic proteins, enabling rapid and robust screening of enzymatic activity, inhibition resistance, and fibrin affinity, stimulation, and selectivity. The outcome translates to key clinical indicators such as biological half‐life and bleeding risk. These assays streamline the development of
Martin Toul, Alan Strunga, Jiri Damborsky, Zbynek Prokop   +3 more
wiley   +1 more source

The Rac1 regulator ELMO controls basal body migration and docking in multiciliated cells through interaction with Ezrin

Development, 2015
Daniel Epting, Krasimir Slanchev, C. Boehlke, Sylvia Hoff, N. Loges, Takayuki Yasunaga, Lara Indorf, S. Nestel, S. Lienkamp, H. Omran, E. Kuehn, O. Ronneberger, G. Walz, A. Kramer-Zucker   +13 more
semanticscholar   +2 more sources

The structure of basal body inner junctions from Tetrahymena revealed by electron cryo-tomography. [PDF]

EMBO J
Li S, Fernandez JJ, Ruehle MD, Howard-Till RA, Fabritius A, Pearson CG, Agard DA, Winey ME.   +7 more
europepmc   +1 more source

Purification and characterization of a basal body-associated Ca2+-binding protein. [PDF]

, 1988
Bo-Wun Huang, D. Martin Watterson, V D Lee, Matthew J. Schibler   +3 more
openalex   +1 more source

Exam‐level analysis of lecture capture viewing and student exam performance

FEBS Open Bio, EarlyView.
This study asks how lecture capture relates to exam performance in biology courses with three noncumulative exams. Mixed‐effects modeling separated between‐students from within‐student effects on exam %. Students who watched more recordings earned higher grades, but viewing spikes above a student's own mean, consistent with cramming, predicted small ...
Kirk Hillsley
wiley   +1 more source

The C-terminus of CFAP410 forms a tetrameric helical bundle that is essential for its localization to the basal body. [PDF]

Open Biol
Stadler A, De Liz LV, Gabriel HB, Alonso-Gil S, Crickley R, Korbula K, Žagrović B, Vaughan S, Sunter JD, Dong G.   +9 more
europepmc   +1 more source

Abnormal basal-body number, location, and orientation in a striated fiber-defective mutant of Chlamydomonas reinhardtii. [PDF]

, 1983
Robin Wright, Bonnie Chojnacki, Jonathan W. Jarvik   +2 more
openalex   +1 more source

Overview of molecular signatures of senescence and associated resources: pros and cons

FEBS Open Bio, EarlyView.
Cells can enter a stress response state termed cellular senescence that is involved in various diseases and aging. Detecting these cells is challenging due to the lack of universal biomarkers. This review presents the current state of senescence identification, from biomarkers to molecular signatures, compares tools and approaches, and highlights ...
Orestis A. Ntintas, Sylvia Vagena, Pavlos Pantelis, Giorgos Theocharous, Russel Petty, Konstantinos Evangelou, Vassilis G. Gorgoulis   +6 more
wiley   +1 more source