Results 321 to 330 of about 31,885,483 (388)

Understanding and measuring mechanical signals in the tumor stroma

FEBS Open Bio, EarlyView.
This review discusses cancer‐associated fibroblast subtypes and their functions, particularly in relation to extracellular matrix production, as well as the development of 3D models to study tumor stroma mechanics in vitro. Several quantitative techniques to measure tissue mechanical properties are also described, to emphasize the diagnostic and ...
Fàtima de la Jara Ortiz, Chiara Cimmino, Maurizio Ventre, Alessandra Cambi   +3 more
wiley   +1 more source

A Multi-Input Neural Network Model for Accurate MicroRNA Target Site Detection. [PDF]

Noncoding RNA
Mohebbi M, Manzourolajdad A, Bennett E, Williams P.   +3 more
europepmc   +1 more source

Expression and purification of E140 protein antigen fragments of Plasmodium vivax and Plasmodium berghei for serological assays

FEBS Open Bio, EarlyView.
We provide a step‐by‐step guide for producing E140 antigen fragments from Plasmodium berghei (Pb1) and Plasmodium vivax (Pv1). Pb1/Pv1 are expressed in E. coli, solubilized by freeze–thawing, refolded by slow dilution, purified by affinity chromatography (IMAC), then concentrated and subjected to quality control.
Rodolfo Ferreira Marques, Edit Ábrahám, Hiromi Muramatsu, Daniel Youssef Bargieri, Norbert Pardi, Zoltán Lipinszki   +5 more
wiley   +1 more source

Systematic analysis of specificities and flanking sequence preferences of bacterial DNA-(cytosine C5)-methyltransferases reveals mechanisms of enzyme- and sequence-specific DNA readout. [PDF]

Nucleic Acids Res
Sogl G, Pilling S, Fischer LFJ, Ludwig J, Mihretu N, Bashtrykov P, Jeltsch A.   +6 more
europepmc   +1 more source

FAM136A depletion induces mitochondrial stress and reduces mitochondrial membrane potential and ATP production

FEBS Open Bio, EarlyView.
FAM136A depletion upregulated ROS production, reduced mitochondrial membrane potential (ΔΨ) and ATP production, and upregulated expression of PCK1, PCK2, HMGCS1, and HMGCS2. The expression of both TOMM22 and TOMM20 was also upregulated. FAM136A depletion reduced HCCS that produce holocytochrome c by combining heme to apocytochrome c, and reduced the ...
Yushi Otsuka, Masato Yano
wiley   +1 more source

Characterization of diverse Cas9 orthologs for genome and epigenome editing. [PDF]

Proc Natl Acad Sci U S A
Butterfield GL, Rohm D, Roberts A, Nethery MA, Rizzo AJ, Morone DJ, Garnier L, Iglesias N, Barrangou R, Gersbach CA.   +9 more
europepmc   +1 more source

Pharmacological effects of osimertinib on a chicken chorioallantoic membrane xenograft model with the EGFR exon‐19‐deleted advanced NSCLC mutation

FEBS Open Bio, EarlyView.
Osimertinib reduces angiogenesis and PDL1 expression in in ovo tumors, transforming them into ‘cold tumors’ with lower immune activity. Anatomopathological and transcriptomic analyses highlight its therapeutic impact on tumor biology. This study underscores osimertinib's potential to reshape the tumor microenvironment and provides insights into its ...
David Barthélémy, Arnaud Vigneron, Xavier Rousset, Jérome Guitton, Emmanuel Grolleau, Margaux Raffin, Julie Balandier, Gaëlle Lescuyer, Mathilde Bardou, Florence Geiguer, Sébastien Couraud, Claire Bardel, Jean Viallet, Nazim Benzerdjeb, Léa Payen   +14 more
wiley   +1 more source

Generating multiple alignments on a pangenomic scale. [PDF]

Bioinformatics
Olbrich J, Büchler T, Ohlebusch E.
europepmc   +1 more source

Tryptophan‐sensing receptor GPR142 expression levels are directly regulated by proinflammatory cytokines in ghrelin‐producing cells

FEBS Open Bio, EarlyView.
GPR142, a tryptophan‐sensing receptor, stimulates gastrointestinal hormones like ghrelin and is linked to inflammation regulation. Proinflammatory cytokines suppress ghrelin production, while tryptophan levels decrease via IDO1 activation. Our findings reveal that proinflammatory cytokines directly regulate GPR142 expression in ghrelin‐producing cells,
Yoko Ueda, Hiroshi Iwakura, Takuya Ensho, Mika Bando‐Shimizu, Asako Doi, Norihiko Matsutani, Shuhei Morita, Hidefumi Inaba, Hiroyuki Ariyasu, Naoki Fukuda, Keiji Hayata, Toshiyasu Ojima, Masahiro Nishi, Taka‐aki Matsuoka, Hiroki Yamaue, Takashi Akamizu   +15 more
wiley   +1 more source

Chemical Evolution of Life on Earth. [PDF]

Genes (Basel)
Lei L, Burton ZF.
europepmc   +1 more source