Results 51 to 60 of about 2,450,393 (299)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Natural vibrations of a beam on stochastic two-layered subsoil with significantly different thickness

Budownictwo i Architektura, 2014
In this paper the influence of variability of Young modulus of the subsoil layers on the natural frequency of the beam-two-layered subsoil system was analyzed. Assuming the first layer was thinner and more rigid then the second one (10 and 20 times). The
Barbara Kaleta, Bartosz Różycki
doaj   +1 more source

Beam-Beam interactions [PDF]

, 2011
In high energy storage-ring colliders, the nonlinear effect arising from beam-beam interactions is a major source that leads to the emittance growth, the reduction of beam life time, and limits the collider luminosity. In this paper, two models of beam-beam interactions are introduced, which are weak-strong and strong-strong beam-beam interactions.
openaire   +2 more sources

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source

Base editing HbS to HbG-Makassar improves hemoglobin function supporting its use in sickle cell disease

Nature Communications
Adenine base editing can convert sickle hemoglobin (HbS, βΕ6V) to G-Makassar hemoglobin (HbG, βE6A), a naturally occurring variant that is clinically asymptomatic.
Zachary Kostamo, Manuel A. Ortega, Chavonna Xu, Patricia R. Feliciano, Elizabeth Budak, Daisy Lam, Valerie Winton, Rebecca Jenkins, Archita Venugopal, Margaret Zhang, John Jamieson, Brent Coisman, Kennedy Goldsborough, Britney Hernandez, Celeste K. Kanne, Erica N. Evans, Jordan Zgodny, Yankai Zhang, Jawa Darazim, Ashwin Patel, Michael A. Pendergast, John Manis, Adam J. Hartigan, Giuseppe Ciaramella, Seung-Joo Lee, S. Haihua Chu, Vivien A. Sheehan   +26 more
doaj   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Base-editing corrects metabolic abnormalities in a humanized mouse model for glycogen storage disease type-Ia

Nature Communications
Glycogen storage disease type-Ia patients, deficient in the G6PC1 gene encoding glucose-6-phosphatase-α, lack blood glucose control, resulting in life-threatening hypoglycemia. Here we show our humanized mouse model, huR83C, carrying the pathogenic G6PC1-
Irina Arnaoutova, Yvonne Aratyn-Schaus, Lisa Zhang, Michael S. Packer, Hung-Dar Chen, Cheol Lee, Sudeep Gautam, Francine M. Gregoire, Dominique Leboeuf, Steven Boule, Thomas P. Fernandez, Victoria Huang, Lo-I Cheng, Genesis Lung, Brianna Bannister, Jeremy Decker, Thomas Leete, Lan S. Shuang, Caroline Bock, Prachi Kothiyal, Phil Grayson, Ka W. Mok, Jeffrey J. Quinn, Lauren Young, Luis Barrera, Giuseppe Ciaramella, Brian C. Mansfield, Janice Y. Chou   +27 more
doaj   +1 more source

Dynamic Stability of the Periodic and Aperiodic Structures of the Bernoulli-Euler Beams [PDF]

Archives of Metallurgy and Materials
The study analyzed the influence of periodic and aperiodic stiffness distribution for the four-element Bernoulli-Euler beam on the first two eigenfrequencies and the dynamic stability of the system. The influence of increasing the ratio of cross-sections
J. Garus, J. Petrů, W. Sochacki, S. Garus   +3 more
doaj   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Universal vortex-state Hall conductivity of YBa2Cu3O7 single crystals with differing correlated disorder [PDF]

, 1998
The vortex-state Hall conductivity ([sigma][sub]xy) of YBa2Cu3O7 single crystals in the anomalous-sign-reversal region is found to be independent of the density and orientation of the correlated disorder.
Beam, D. A., Holtzberg, F., Yeh, N.-C.
core